Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods

Abstract

DOI: 10.26650/EuroJBiol.2018.404499

Objective: In this study, the diagnosis of infection caused by Vibrio anguillarum in cultured rainbow trout (Oncorhynchus mykiss), in Turkey using bacteriological, histopathological and immunohistochemical methods was attempted.

Materials and Methods: 15 rainbow trout samples showing signs of disease were investigated using bacteriological and histopatological methods.

Results: Because of physiological and biochemical characteristics of bacteria growing on the medium, these bacteria were isolated and identified as V. anguillarum. As a result of the histopathologic examination of the tissues of diseased rainbow trout, hyperemia in the liver, degeneration of the epithelium of kidney tubules and necrosis in the interstitial area, enlargement in the gill filaments, cells having picnotic nucleus  and several necrotic areas in the spleen were observed. It was also detected in the streptavidin-biotin staining method that the causative agent accumulates in the liver, spleen, kidney and also in the gills especially in the areas near around the blood vessels depending on the development stage of the disease.

Conclusion: As a result, an immunoenzimatic method, streptavidin-biotin staining method was used in this study for the detection of the presence of  V. anguillarum in the infected rainbow trout tissues.

Keywords

• Rainbow trout,Vibrio anguillarum,histopathology,streptavidin-biotin

Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods

Abstract

DOI: 10.26650/EuroJBiol.2018.404499

Objective: In this study, the diagnosis of infection caused by Vibrio anguillarum in cultured rainbow trout (Oncorhynchus mykiss), in Turkey using bacteriological, histopathological and immunohistochemical methods was attempted.

Materials and Methods: 15 rainbow trout samples showing signs of disease were investigated using bacteriological and histopatological methods.

Results: Because of physiological and biochemical characteristics of bacteria growing on the medium, these bacteria were isolated and identified as V. anguillarum. As a result of the histopathologic examination of the tissues of diseased rainbow trout, hyperemia in the liver, degeneration of the epithelium of kidney tubules and necrosis in the interstitial area, enlargement in the gill filaments, cells having picnotic nucleus  and several necrotic areas in the spleen were observed. It was also detected in the streptavidin-biotin staining method that the causative agent accumulates in the liver, spleen, kidney and also in the gills especially in the areas near around the blood vessels depending on the development stage of the disease.

Conclusion: As a result, an immunoenzimatic method, streptavidin-biotin staining method was used in this study for the detection of the presence of  V. anguillarum in the infected rainbow trout tissues.

Keywords

• Rainbow trout,Vibrio anguillarum,histopathology,streptavidin-biotin

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