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The changes of oxidative stress and Δ9-tetrahydrocannabinol accumulation in liver of type-2 diabetic rats

Yıl 2015, Cilt: 74 Sayı: 2, 1 - 8, 19.03.2017

Öz








In this study, we aimed to explore the changes of oxidative stress, Δ9- tetrahydrocannabinol (Δ9-THC)
and its metabolites accumulation in the liver of type-2 diabetic rats treated with Δ9-THC. 8-10 week-old
Sprague-Dawley rats were divided into four groups. Group I: Physiological saline was administered
intraperitoneally(i.p) (n=7). Group II: Rats that was given Δ
9-THC for 7 days (3 mg/kg/day) (n=6)
(i.p). Group III: Streptozotocin(STZ, 65 mg/kg)+Nicotinamide(NAD, 85 mg/kg) (n=7) (i.p). Group IV:
Diabetic rats that were given Δ
9-THC(3 mg/kg/day) for 7 days (n=7) (i.p). The biochemical investigation
was carried out on the serum and liver tissue. Δ
9 -THC and its metabolites were analyzed by using GC-
MS in the liver of rats. Liver glutathione level in diabetes+ Δ
9-THC increased as compared to diabetic
rats. The increased lipid peroxidation and protein carbonyl levels in diabetes showed a low reduction
with Δ
9-THC. Catalase and superoxide dismutase activities in liver of diabetic rats increased with Δ9-
THC treatment, non-statistically. Serum uric acid level, alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) activities slightly increased in diabetic group as compared to control group.
While the AST level reduced in the liver, ALT level did not be affected in Δ
9-THC treated with diabetic
rats as compared with the diabetic group. Δ
9-THC level in liver was negative in given Δ9-THC groups.
But the liver THC-COOH metabolite accumulation in Δ
9-THC group is higher than Diabetes + Δ9-THC
group. We could say that Δ9-THC can reduce diabetes-induced oxidative damage in the liver of type-2
diabetic rats. The metabolization of Δ
9-THC in the liver can be accelerated with diabetes. 




Kaynakça

  • Aebi H. (1984) Catalase in vitro. Methods Enzymol, 105: 121–6.
  • Ahmed S.A., Ross S.A., Slade D., Radwan M.M., Khan I.A. and ElSohly M.A. (2015) Minor oxygenated cannabinoids from high potency Cannabis sativa L. Phytochemistry, 117: 194-9.
  • Arulselvan P. and Subramanian S.P. (2007) Bene cial effects of Murraya koenigii leaves on antioxidant defense system and ultrastructural changes of pancreatic β
  • cells in experimental diabetes in rats. Chem Biol Interact, 165: 155–64.
  • Ávila Dde L., Araújo G.R., Silva M., Miranda P.H., Diniz M.F., Pedrosa M.L., Silva M.E.,de Lima W.G. and Costa D.C. (2013) Vildagliptin ameliorates oxidative stress and pancreatic beta cell destruction in type 1 diabetic rats. Arch Med Res, 44: 194–202.
  • Baburao Jain A. and Anand J.V. (2012) Vitamin E, its bene cial role in diabetes ellitus (DM) and its complications. J Clin Diagn Res, 6: 1624–8.
  • Beutler E., Duron O. and Kelly B.M. (1963) Improved method for the determination of blood glutathione. J Lab Clin Med, 51: 882-8.
  • Brenneisen R., Meyer P., Chtioui H., Saugy M. and Kamber M. (2010) Plasma and urine pro les of Delta9-tetrahydrocannabinol and its metabolites 11-hydroxy-Delta9 tetrahydrocannabinol and 11-nor-9- carboxy-Delta9-tetrahydrocannabinol after cannabis smoking by male volunteers to estimate recent consumption by athletes. Anal Bioanal Chem, 396(7): 2493-502.
  • Chen J., Lee C.T., Errico S., Deng X., Cadet J.L. and Freed W.J. (2005) Protective effects of Delta(9)-tetrahydrocannabinol against N-methyl-d-aspartate-induced AF5 cell death. Brain Res Mol Brain Res, 134(2): 215-25.
  • Coskun Z.M., Sacan O., Karatug A., Turk N., Yanardag R., Bolkent S. and Bolkent S. (2013) Regulation of oxidative stress and
Yıl 2015, Cilt: 74 Sayı: 2, 1 - 8, 19.03.2017

Öz

Kaynakça

  • Aebi H. (1984) Catalase in vitro. Methods Enzymol, 105: 121–6.
  • Ahmed S.A., Ross S.A., Slade D., Radwan M.M., Khan I.A. and ElSohly M.A. (2015) Minor oxygenated cannabinoids from high potency Cannabis sativa L. Phytochemistry, 117: 194-9.
  • Arulselvan P. and Subramanian S.P. (2007) Bene cial effects of Murraya koenigii leaves on antioxidant defense system and ultrastructural changes of pancreatic β
  • cells in experimental diabetes in rats. Chem Biol Interact, 165: 155–64.
  • Ávila Dde L., Araújo G.R., Silva M., Miranda P.H., Diniz M.F., Pedrosa M.L., Silva M.E.,de Lima W.G. and Costa D.C. (2013) Vildagliptin ameliorates oxidative stress and pancreatic beta cell destruction in type 1 diabetic rats. Arch Med Res, 44: 194–202.
  • Baburao Jain A. and Anand J.V. (2012) Vitamin E, its bene cial role in diabetes ellitus (DM) and its complications. J Clin Diagn Res, 6: 1624–8.
  • Beutler E., Duron O. and Kelly B.M. (1963) Improved method for the determination of blood glutathione. J Lab Clin Med, 51: 882-8.
  • Brenneisen R., Meyer P., Chtioui H., Saugy M. and Kamber M. (2010) Plasma and urine pro les of Delta9-tetrahydrocannabinol and its metabolites 11-hydroxy-Delta9 tetrahydrocannabinol and 11-nor-9- carboxy-Delta9-tetrahydrocannabinol after cannabis smoking by male volunteers to estimate recent consumption by athletes. Anal Bioanal Chem, 396(7): 2493-502.
  • Chen J., Lee C.T., Errico S., Deng X., Cadet J.L. and Freed W.J. (2005) Protective effects of Delta(9)-tetrahydrocannabinol against N-methyl-d-aspartate-induced AF5 cell death. Brain Res Mol Brain Res, 134(2): 215-25.
  • Coskun Z.M., Sacan O., Karatug A., Turk N., Yanardag R., Bolkent S. and Bolkent S. (2013) Regulation of oxidative stress and
Toplam 10 adet kaynakça vardır.

Ayrıntılar

Bölüm Makaleler
Yazarlar

Zeynep Mine Coşkun

Yayımlanma Tarihi 19 Mart 2017
Gönderilme Tarihi 19 Mart 2017
Yayımlandığı Sayı Yıl 2015 Cilt: 74 Sayı: 2

Kaynak Göster

AMA Coşkun ZM. The changes of oxidative stress and Δ9-tetrahydrocannabinol accumulation in liver of type-2 diabetic rats. Eur J Biol. Mart 2017;74(2):1-8.