An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats

Year 2017, Volume: 76 Issue: 1, 14 - 19, 28.12.2017

Tunc Catal Sevim Tunali , Sehnaz Bolkent Refiye Yanardag

https://doi.org/10.5152/EurJBiol.2017.1703

Abstract

The
protective effects of a combination of antioxidants on histopathological
changes and biochemical parameters were examined in D-galactosamine
(D-GaIN)-induced hepatotoxicity in rats. Physiological saline solution was
injected intraperitoneally into the control group, while D-GaIN (500 mg kg-1)
was administered intraperitoneally into the experimental animals. The
combination of 100 mg/kg/day ascorbic acid, 100 mg/kg/day alpha tocopherol, 15
mg/kg/day beta carotene, and 0.2 mg/kg/day sodium selenate was administered
orally to intact control rats for 3 days. The same antioxidant combination was
given to the D-GaIN group. Liver and blood samples were used for
histopathological and biochemical assays. Liver tissues were significantly
damaged by D-GaIN administration based on the histopathological findings. Serum
aspartate and alanine transaminase, gamma glutamyl transpeptidase, alkaline
phosphatase, lactate dehydrogenase, sialic acid, and uric acid levels
increased. Serum and liver glutathione levels decreased, and serum superoxide
dismutase, glutathione peroxidase, and glutathione-S-transferase, catalase
activities as well as lipid peroxidation values increased in the D-GaIN group.
In contrast, administration of the antioxidants reversed the histopathological
and biochemical changes in the liver of animals administered D-GaIN. In
conclusion, the administration of a combination of antioxidants suppressed
histopathological changes and biochemical parameters in rats given D-GaIN.

Keywords

Vitamin C, β-carotene, D-galactosamine, sodium selenate, vitamin E, liver damage

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