The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells

Year 2018, Volume: 77 Issue: 1, 32 - 37, 22.06.2018

Canan Eroglu Ebru Avci Mucahit Secme Yavuz Dodurga Hasibe Vural Ercan Kurar

Abstract

DOI:
10.26650/EuroJBiol.2018.0003

Objective: Prostate cancer is the second
most common cause of cancer-related deaths in men. Nowadays, new treatment
approaches have been tested for cancer therapy including natural compounds with
low toxicity. Ferulic acid (FA) is known as an abundant phenolic compound found
in various fruits and vegetables. As a potent antioxidant, the anticarcinogenic
effect of FA has been demonstrated in various cancer cell lines. The objective
of this study was to investigate the combined effect of FA and gemcitabine on
apoptosis and metastasis in PC-3 human prostate cancer cell lines.

Materials and Methods: Cell viability was
determined using the XTT method after the cells were treated with gemcitabine
or FA and gemcitabine. According to the results of cytotoxicity assays, PC-3
cells were treated with <IC50 doses of combination (200 μM FA and 35 μM
gemcitabine) and IC50 dose of gemcitabine. Expressions of genes that are
important in apoptosis and metastasis pathways were evaluated in dose and
control groups by qPCR.

Results: According to the results, the combination
of FA and gemcitabine affected the expression of more genes in apoptosis and
metastasis with a higher fold change compared with the single treatment of
gemcitabine in PC-3 human prostate cancer cell lines.

Conclusion: Our study indicates that FA can
be an effective part of the combination treatments.

Keywords

Ferulic acid, gemcitabine, PC-3 cell line

The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells

Abstract

DOI: 10.26650/EuroJBiol.2018.0003

Objective: Prostate cancer is the second most common cause of cancer-related deaths in men. Nowadays, new treatment approaches have been tested for cancer therapy including natural compounds with low toxicity. Ferulic acid (FA) is known as an abundant phenolic compound found in various fruits and vegetables. As a potent antioxidant, the anticarcinogenic effect of FA has been demonstrated in various cancer cell lines. The objective of this study was to investigate the combined effect of FA and gemcitabine on apoptosis and metastasis in PC-3 human prostate cancer cell lines.

Materials and Methods: Cell viability was determined using the XTT method after the cells were treated with gemcitabine or FA and gemcitabine. According to the results of cytotoxicity assays, PC-3 cells were treated with <IC50 doses of combination (200 μM FA and 35 μM gemcitabine) and IC50 dose of gemcitabine. Expressions of genes that are important in apoptosis and metastasis pathways were evaluated in dose and control groups by qPCR.

Results: According to the results, the combination of FA and gemcitabine affected the expression of more genes in apoptosis and metastasis with a higher fold change compared with the single treatment of gemcitabine in PC-3 human prostate cancer cell lines.

Conclusion: Our study indicates that FA can be an effective part of the combination treatments.

Keywords

Ferulic acid, gemcitabine, PC-3 cell line

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