Anatomy and Histology of Digestive Tract in Melanophila (Trachypteris) picta decastigma (Fabricius, 1787) (Coleoptera: Buprestidae)

Year 2021, Volume: 80 Issue: 1, 1 - 8, 15.06.2021

Nurcan Özyurt Koçakoğlu , Üzeyir Çağlar , Selami Candan

Abstract

Objective: The aim of this study is to investigate the anatomy and histology of Melanophila picta decastigma (Fabricius) (Coleoptera: Buprestidae).

Materials and Methods: Some of the samples were fixed in 10% formaldehyde for light microscopy, others were fixed with 2.5% glutaraldehyde for scanning electron microscopy (SEM). Then the samples were examined by using stereo, light, and SEM.

Results: The digestive tract in M. picta decastigma consists of foregut, midgut, and hindgut. The foregut is composed of the pharynx, esophagus, crop and proventriculus. The foregut showed muscle layers followed by intima and epithelium. There is a long, tubular pair of gastric caeca in the anterior part of the midgut. In cross-sections, a single-layered cylindrical epithelium with a short striated border. The midgut showed that the epithelial lining was substantially folded, giving its lumen a curved profile. These monolayered columnar epithelial cells had their nuclei located medially, and apically they have a striated border. The midgut surface was covered by rounded structures called regenerative crypts. The main sections of hindgut (pyloric valve, ileum, colon and rectum) were clearly observed. In the hindgut sections, there appeared to be a highly folded cuticular lining, a monolayer cubic epithelium and a very muscular wall.

Conclusions: Studies on the digestive tract histo-anatomy of M. picta decastigma will add more information to assist in experimental, taxonomical, histological, and anatomical studies about insect tissues and will serve as a basis for control studies.

Keywords

foregut, midgut, hindgut, Malpighian tubules, light microscope, electron microscope

Thanks

We express our thanks to Gazi University Academic Writing and Research Center for their help and support in the proofreading of the current study.

References

• 1. Bellamy CL A catalogue of the higher taxa of the family Buprestidae (Coleoptera). Navorsinge van die Nasionale Museum, Bloemfontein, 1985; 4(15): 405-72.
• 2. Bíly S. Summary of the bionomy of the buprestid beetles of central Europe (Coleoptera: Buprestidae). Acta Entomologica Musaei Nationalis Pragae, Supplement, 2002; 10: 1-104.
• 3. Özyurt Koçakoğlu N, Candan S, Çağlar Ü. Histomorphology of the Adult Digestive Tract of Capnodis tenebrionis (L. 1758) (Coleoptera, Buprestidae). Microscopy and Microanalysis, 26(6): 1245-54. doi:10.1017/S1431927620024472
• 4. Foottit RG, Adler, PH. Insect Biodiversity: Science and Society, Blackwell Publishing, West Sussex, UK, 2009.
• 5. Sakalian V, Langourov M. Color trap a method for distributional and ecological investigations of Buprestidae (Coleoptera). Acta Soc. Zool. Bohem. 2004; 68, 53–9.
• 6. Çanakçıoğlu, H. Orman Entomolojisi. İ.Ü. Orman Fakültesi Yayınları, İ.Ü. Yay. No: 3152, Orman Fak. Yay. No: 349, İstanbul, Birinci baskı, Matbaa Teknisyenleri Basımevi; 1983.
• 7. Schmitz H, Bousack H. Modelling a historic oil-tank fire allows an estimation of the sensitivity of the infrared receptors in Pyrophilous melanophila beetles. Plos One. 2012; 7 (5): e37627.
• 8. Bily S. The Buprestidae (Coleoptera) of Fennoscandia and Denmark. Fauna Entomologica Scandinavica, 1982; 10; pp 109.
• 9. Evans HF, Moraal LG, Pajares JA. Biology, Ecology and Economic Importance of Buprestidae and Cerambycidae. Lieutier KRD F, Battisti A, Grégoire J-C, Evans HF, editors. Bark and Wood Boring Insects in Living Trees in Europe, A Synthesis. Dordrecht: Springer; 2007. pp. 447-74.
• 10. Liu XD, Jia XZ. A grey related analysis Cytospora chrysosperma with Melanophila decastigma of poplar, Forest Pest and Disease, 1988; 4: 26-7.
• 11. Khabir ZH, & Sadeghi, S. E. (2012). Determination of Melanophila picta Pall.(Coleoptera: Buprestidae) damage rate in Populus alba/Medicago sativa agroforestry system. Mun Ent Zool, 7(2): 920-25.
• 12. Borges I, Nóia M, Camarinho R, Rodrigues AS, Soares AO. Characterization of the alimentary canal of the aphidophagous ladybird, Adalia bipunctata (Coleoptera: Coccinellidae): anatomical and histological approaches. Entomol.Sci; 2015; 18(1): 66-73.
• 13. Chapman RF. Comprehensive and Insect Physiology. Kerkut GA, Gilbert LI editors. Biochemistry and Pharmacology. Oxford Pergamon, 1985.
• 14. Dow JA. Insect midgut function. Advances in Insect Physiol 1986; 19; 187-328.
• 15. Gullan DJ, Cranston PS. The Insects: An Outline of Entomology, 3rd edn, Blackwell Publishing Ltd, Melbourne, 2005.
• 16. Wigglesworth VB. The Principles of Insect Physiology. Chapman and Hall Ltd, London, 1977.
• 17. Vazquez-Arista M. Anatomical, Enzymatic, and Microbiological Studies on the Digestive System of Prostephanus truncatus (Horn), Doctoral thesis, University of Leicester, England, 1997.
• 18. Gardner JA. Revision of the higher categories of Stigmoderini (Coleoptera: Buprestidae), The University of Adelaide, South Australia, Doctoral thesis, 1986.
• 19. Bection Jr, EM. The alimentary tract of Phanaeus vindex Macl.(Scarabaeidæ). Ohio J Sci 1930; 30(5): 315-23.
• 20. Brubaker RW. The alimentary tract of Penthe Pimelia, Fabr.(Coleoptera: Dacnidae). Ohio J Sci 1934; 34(1): 46-56.
• 21. Everly RT 1936. The alimentary tract of the margined blister beetle, Epicauta cinerea marginata Fab.(Coleoptera-Meloidae). Ohio J Sci 36(4): 204-16.
• 22. Davidson RH. The alimentary canal of Criocerus asparagi Linn. Ohio J Sci 1931; 31(5): 396-405.
• 23. Fletcher, F. W. (1930). The alimentary canal of Phyllophaga gracilis Burm. Ohio J Sci 30(2): 109-19.
• 24. Lewis HC. The alimentary canal of Passalus. Ohio J Sci 1926; 26(1): 11-24.
• 25. Bigham, JT. The alimentary canal of Asaphes memnonius Hbst. Ohio J Sci 1931; 31(5): 386-95.
• 26. Díaz E, Arciniega O, Sánchez L, Cisneros R, Zúñiga G. Anatomical and histological comparison of the alimentary canal of Dendroctonus micans, D. ponderosae, D. pseudotsugae pseudotsugae, D. rufipennis, and D. terebrans (Coleoptera: Scolytidae). Ann Entomol Soc Am 2003; 96 (2): 144-52.
• 27. Sarwade AB, Bhawane GP. Anatomical and histological structure of alimentary canal of adult Platynotus belli (Coleoptera: Tenebrionidae). BFAIJ 2013; 5:47-55.
• 28. Calder AA. The alimentary canal and nervous system of Curculionoidea (Coleoptera): Gross morphology and systematic significance. J Nat Hist 1989; 23(6): 1205-65.
• 29. Candan S, Özyurt Koçakoğlu N, Güllü M, Çağlar Ü. Anatomical and histological studies of the alimentary canal of adult maize leaf weevil, Tanymecusdilaticollis Gyllenhal, 1834 (Coleoptera: Curculionidae). Microsc Res Tech; (2020). 83: 1153-62.
• 30. Opitz W. Morphologic studies of the alimentary canal and internal reproductive organs of the Chaetosomatidae and the Cleridae (Coleoptera: Cleroidea) with comparative morphology and taxonomic analyses. Insecta Mundi, 2014; 0342: 1-40.
• 31. Candan S, Özyurt Koçakoğlu N, Erbey M. Morphology and histology of the alimentary canal of Epiphaneus malachiticus Boheman, 1842 (Coleoptera, Curculionidae). Entomol Rev 2019; 99(3): 326-36.
• 32. Bu SH, Chen H. The alimentary canal of Dendroctonus armandi Tsai and Li (Coleoptera: Curculionidae: Scolytinae). Coleopts Bull 2009; 63(4): 485-96.
• 33. De Sousa G, Scudeler EL, Abrahão J, Conte H. Functional morphology of the crop and proventriculus of Sitophilus zeamais (Coleoptera: Curculionidae). Ann Entomol Soc Am 2013; 106(6): 846-52.
• 34. Rubio JDG, Bustillo PAE, Vallejo ELF, Acuña ZJR, Benavides MP Alimentary canal and reproductive tract of Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae, Scolytinae). Neotrop Entomol 2008; 37(2): 143-51.
• 35. Aldigail SA, Alsaggaff AI, Al-Azab AM. Anatomical and histological study on the digestive tract of Epilachna chrysomelina (Coleoptera: Coccinellidae). Biosci Biotechnol Res 2013; 10 (1): 183-92.
• 36. McAllister JC, Steelman CD, Carlton CE. Histomorphology of the larval and adult digestive systems of Alphitobius diaperinus (Coleoptera: Tenebrionidae). J Kansas Entomol Soc, 1995; 195-205.
• 37. Bess HA. The alimentary canal of Calosoma sycophanta Linnaeus. Ohio J Sci 1935; 35: 54-61.
• 38. Díaz E, Cisneros R, Zúñiga G. Comparative anatomical and histological Study of the alimentary canal of the Dendroctonus frontalis (Coleoptera: Scolytidae) Comp Ann Entomol Soc Am 2000; 93(2): 303-11.
• 39. Singh OL, Prasad B. Histomorphology of the alimentary tract of adult, Odoiporus longicollis (Oliv.) (Coleoptera: Curculionidae). J Entomol Res Soc 2013; 1:109-115.
• 40. Sinha, RN. The alimentary canal of the adult of Tribolium castaneum Herbst (Coleoptera, Tenebrionidae). J Kansas Entomol Soc, 1958; 31 (2), 118-25.