Using Chloroplast Regions accD, matK, rbcL, and ycf-1 for Phylogeny Construction in Polyspora huongiana Orel, Curry & Luu

Year 2024, Volume: 83 Issue: 2, 160 - 172, 19.12.2024

Nguyen Quan , Hoang Thanh Chi , Phung My Trung , Truong Quang Cuong , Tran Thi , Hoang Son , Bui Thi Kim Ly

https://doi.org/10.26650/EurJBiol.2024.1447284

Abstract

Objective: Theaceae is a commodity with high economic value. The diversity of Theaceae species present in Southeast Asia, especially Vietnam, provides an excellent supply source for promoting the development of related industries. Nearly 130 species of Theaceae, many of which are highly endemic, were discovered in Vietnam, including 13 Polyspora species. Polyspora huongiana was discovered in Bidoup-Nui Ba National Park, Vietnam, on January 7, 2010. Because they are native species and lack data, studies are needed to provide genetic data for ecological assessment and original identification of the plant.

Materials and Methods: Sanger sequencing, data collection, and nucleotide analysis of the genetic data of accD, matK, rbcL, and ycf1 in P. huongiana were provided.

Results: The results showed that combinations of two sequences could separate the Polyspora genus, whereas at least three sequences were necessary to identify P. huongiana, which was genetically closely related to Polyspora axillaris and Polyspora hainanensis.

Conclusion: P. huongiana is closely related to Polyspora axillaris and Hainanensis. The combination of 3–4 sequences allowed reliable identification of P. huongiana.

Keywords

Polyspora huongiana, Phylogeny, matK, rbcL, accD, ycf1

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