Cytogenetic Studies in Some Species of Medicago L. in Iran

Year 2014, Volume: 73 Issue: 1, 21 - 30, 27.11.2014

Sara Sadeghian , Seyed Hesamzadeh Hejazi

Abstract

A karyological study using the Image Analysis System was conducted of eight taxa of the genus
Medicago L. namely M. radiata L., M. intertexta (L.) Mill. M. orbicularis (L.) Bart. , M. laciniata (L.)
Mill., M. coronate (L.) bartal, M. rigidula (L.) All., M. polymorpha L.and M. scutellata (L.) Mill. used
as forage plants from different geographic origins of Fars province from Iran. We found the two usual
basic chromosome numbers in the genus, x=7 and x=8. In the group with x=7, two diploid (2n=14), one
tetraploid (2n=28) species and in the group with x=8, five diploid (2n=16) species were found. Detailed
karyotype analysis allows us to group the different species and to postulate relationships among them.

Keywords

Chromosome, Fabaceae, karyology, Medicago, taxonomy

References

• Abdelguerfi A. and Guittonneau G. G. (1979) Reports. [In: Uive, A. (ed.), IOPB chromosome number reports LXIVTaxon 28: 392-393.
• Cannon S.B., Sterck L., Rombauts S., Sato S., Cheung F. Gouzy J.P., Wang X., Mudge J., Vasdewani J., Schiex T., Spannagl M., Monaghan E., Nicholson C., Humphray S.J., Schoof H., Mayer K.F.X., Rogers J., Quetier F., Oldroyd G.E., Debelle F., Cook D.R., Roe B.A., Town C.D., Tabata S., Van de Peer Y., Young N.D. (2006) Legume evolution viewed through Abdelguerfi the Medicago truncatula and Lotus japonicus genomes. Proceedings National Academy Sciences USA, 103:14959-14964.
• Dahlgren R., Karlsson T. and Lassen P. (1971) Studies on the Flora of the Balearic Islands I -Chromosome numbers in Balearic Angiosperms. Bot. Not. (Lund), 124: 249- 269.
• Darlington C.D. and Wylie A.P. (1961) Chromosome atlas of flowering plants. George Allen G. Unwin Ltd London UK.
• Farsi M. (1989) Cytological study of some Medicago species and polypoloidy effects on them. M.Sc. dissertation, the University of Tabriz, college of Agriculture.
• Femandes A. and Queiros M. (1978) Contribution à la connaissance cytotaxonomique des Spermatophyta du Portugal. IV. Leguminosae (Suppl. 3), Boletim da Sociedade Broteriana, 52: 79-1 64.
• Femandes A. and Santos M. F. (1971) Contribution à la connaissance cytotaxonomique des Spermatoph yta du Portugal. IV. Leguminosae, Boletim da Sociedade Broteriana, 45 (se r. 2): 177-226.
• Femandes A. and Queiros M. (1977) Contribution à la connaissance cytotaxonomique des Spermatophyta du Portugal. Leguminosae, Boletim da Sociedade Broteriana,51: 137- 186.
• Gazanchian A. (1993) Morphological and cytological study of annual medic of Khorasan province. M.Sc. dissertation. Islamic Azad university, Karaj college of Agriculture. 182 p.
• Goldblatt P. (1981) Index to plant chromosome numbers 1975- 1978. Monogr. Syst. Missouri Botanical Garden, U.S.A, 5.
• Goldblatt, P. (1988) lndex to plant chromosome numbers 198 4-1 985. Monogr. Sys t. Bot. Missouri Botanical Garden, U.S.A, 23
• Hesamzadeh Hejazi S.M., Rasuli M. (2006) Cytogenetic study of some species of Vetch Genus (Vicia sp) in Iran. Iranian Journal of Agriculture Science, 37-1(2): 213-225.
• Hesamzadeh Hejazi S.M., Ziaei Nasab M. (2009) Cytogenetic study on several populations of diploid species of Onobrychis in natural gene bank of Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 16(2): 158-171.
• Hesamzadeh Hejazi S.M., Ziaei Nasab M. (2010) Cytaxonomy of some Onobrychis (Fabaceae) species and populations in Iran. Caryologia, 63, 18-31.
• Hesamzadeh Hejazi S.M. (2011) Karyological study on three Cicer L. species (Fabaceae) in Iran.Asian Journal. of cell Biology, 6(3):97-104.
• Heyn C.C. (1963) The annual species of Medicago, Vol XII, Jerusalem, pp 125.
• Heyn C.C. (1984) Medicago in, K. H. Rechinger (Ed.), Flora Iranica157; Axademische Druch-U. verlagsanstalt, Graz. pp 253-271.
• Irani P., Hesamzadeh Hejazi S.M., Tabaei aghdaei S.R. (2014) Karyological study on four species of Satureja (Lamiaceae) in Iran. International Journal of Biosciences (IJB), 4:7,229-240.
• Irani P., Hesamzadeh Hejazi S.M., Tabaei aghdaei S.R. (2014) Karyological study on four species of satureja (Lamiaceae) in Iran. International Journal of Biosciences (IJB), 4:7,229-240.
• Javadi H., Hesamzadeh Hejazi S.M, Babayev Majnun S.H. (2009) Karyotypic Studies of three Thymus (Lamiaceae) species and populations in Iran. Caryologia 62(4), 316- 325.
• JMP. (1995) JMP/STAT for windows, version 3.1.2. SAS Institute Inc.
• Karadag Y. and Gulcan H. (1997) Researchor some cytological characters of some medic species (M. sativa, M. orbicularis, M. polymorpha) occurring in natural vegetation of the Cukurova region. Turkish Jornal of Agriculture and Forestry. 21:2, 121-127.
• Labadie j .P. (1979) Etude caryosystématique de quelques espèces de la nore d’AIgérie. -Naturalia Monspel., Sér. Bot. 32: I- II.
• Labadie j. P. (1980) Reports [In: La ve, A. (ed.), IOPB chromosome number reports LXV]. Taxon 28: 628-629.
• Lesins K.A., Lesins I. and Gillies C.B. (1970) Medicago murex with 2n=14 and 2n=16 chromosomes complements.Chromosoma, 30:109-122
• Lesins K.A., Lesins I. (1979) Genus Medicago (Leguminosae). A taxogenetic study. Dr. W. Junk, publishers, The Hague, pp A. and
• Guittonneau G. G. (1979) Reports. [In: Uive, A. (ed.), IOPB chromosome number reports LXIV- Taxon 28: 392-393.
• Levan A.K., Fredga K., Sandberg A.A. (1964) Nomenclature for centromic position on chromosomes. Hereditas, 52: 201-220.
• Luque T., Mejias J.A. and Diaz Lifante Z. (1988) Numeros cromosomicos para latlo ra Espanola. 544-550. Lagascal ia 15: 130- 133.
• Nilsson O. and Lassen P. (1971) Chromosome Numbers of Vascular Plants from Austria, Mallorca and Yugos lavia. Bot. Not., 124: 270-276.
• Nixon K. (2006) Diversity of life .org (DOL), Cornell University, from http://www. Plantsystematics.org.
• Rechinger K.H. (1984) Onobrychis in Flora Iranica.Akademische Druck And Verlagsanstalt, Graz- Austria,157: 387-484.
• Reeves A., Tear J., Coloradostate university, (1997-2000) from http://www.colostate. edu/Depts/Biology/micromeasure
• Romero Zarco C. (1986) A new method for estimating Karyotype asymmetry. Taxon, 36: 526-530.
• Salehi M., Hesamzadeh Hejazi S.M., Tabaei aghdaei S.R. (2014) Cytogenetic studies of two Dracocephalum (Lamiaceae) species and populations in Iran. International Journal of Biosciences (IJB), 4:9,100-108.
• SAS (1996) SAS/STAT for windows, version 6.12. SAS Institute Inc, Cary,NC 27513, USA
• Schlarbaum S. E., Small E. and John son L. B. (1984) Karyotypic evolution, morphological variability and phylogeny in Medicago sect. lnten extae. Plant Systematic Evolution, 145: 169-181.
• Schultz O. F. (1901) Monographie der Gattung Melilotus. Botanische Jahrbücher für Systematik, Pflanzengeschichte und Pflanzengeographie 29:660–735.
• Shariat A. (2001) Genetic diversity of annual medics based on cytological, electrophoresis and morphological studies. M.Sc. dissertation, the university of Sistan and Balluchestan, Zabol college of Agriculture. 175 p.
• Small E., Lassen P., Brookes B. (1987) An expanded circumscription of Medicago (Leguminosae, Trifolieae) based on explosive flower tripping. Willdenowia 16: 415–437.
• Small E. Brookes B. and Crawford E.J. (1990) Intercontinental differentiation in Medicago rigidula. Canadian Journal of Botany 68:2607-2613.
• Statisti XL (2007) Statistical for windows, version 1.7. University of Western Australia. from http://www.statistixl.com.
• Stebbins G.L. (1971) Chromosomal evolution in higher plants. Edward Arnold publisher, London,Ltd. 216.