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Yıl 2015, Cilt: 45 Sayı: 2, 165 - 179, 22.11.2016

Öz

Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism

Yıl 2015, Cilt: 45 Sayı: 2, 165 - 179, 22.11.2016

Öz

Abstract: The yeast Saccharomyces cerevisiae is a simple eukaryote and an excellent model organism for molecular biology. In this study, a NaCl-resistant S. cerevisiae mutant obtained by inverse metabolic engineering was used as a model to investigate responses and resistance behavior to NaCl, LiCl, KCl, TMA, spermine and sorbitol stresses., at physiological and genetic levels. The physiological spot test results revealed that the NaCl-resistant yeast mutant showed higher resistance to LiCl and NaCl. Gene expression analysis by qRT-PCR revealed that ENA6 and NHA1 genes of the mutant were induced in the absence and presence of LiCl and NaCl. The dysfunction of Na+/H+ antiporters are related to several diseases such as hypertension, epilepsy, postischemic myocardial arrhythmia, gastric and kidney disease, diarrhea, and glaucoma. Thus, the NaCl-resistant yeast mutant could be used to understand cellular sodium and lithium resistance mechanisms and the function of Na+/H+ antiporters also in higher eukaryotic organisms, including humans.
Key words: Saccharomyces cerevisiae, stress resistance, salt-resistance , lithium resistance, NHA1

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Ayrıntılar

Bölüm Araştırma Makalesi
Yazarlar

Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2 Carola Hunte 4, Z. Petek Çakar1,2

Yayımlanma Tarihi 22 Kasım 2016
Yayımlandığı Sayı Yıl 2015 Cilt: 45 Sayı: 2

Kaynak Göster

APA Carola Hunte 4, Z. Petek Çakar1,2, Ş. H. T. 1. A. 1. (2016). Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University, 45(2), 165-179.
AMA Carola Hunte 4, Z. Petek Çakar1,2 ŞHT1A1. Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University. Ocak 2016;45(2):165-179.
Chicago Carola Hunte 4, Z. Petek Çakar1,2, Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2. “Physiological and Genetic Analysis of Cellular Sodium and Lithium response/Resistance Behavior Using the Yeast Saccharomyces Cerevisiae As a Model Organism”. Journal of Faculty of Pharmacy of Istanbul University 45, sy. 2 (Ocak 2016): 165-79.
EndNote Carola Hunte 4, Z. Petek Çakar1,2 ŞHT1A1 (01 Ocak 2016) Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University 45 2 165–179.
IEEE Ş. H. T. 1. A. 1. Carola Hunte 4, Z. Petek Çakar1,2, “Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism”, Journal of Faculty of Pharmacy of Istanbul University, c. 45, sy. 2, ss. 165–179, 2016.
ISNAD Carola Hunte 4, Z. Petek Çakar1,2, Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2. “Physiological and Genetic Analysis of Cellular Sodium and Lithium response/Resistance Behavior Using the Yeast Saccharomyces Cerevisiae As a Model Organism”. Journal of Faculty of Pharmacy of Istanbul University 45/2 (Ocak 2016), 165-179.
JAMA Carola Hunte 4, Z. Petek Çakar1,2 ŞHT1A1. Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University. 2016;45:165–179.
MLA Carola Hunte 4, Z. Petek Çakar1,2, Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2. “Physiological and Genetic Analysis of Cellular Sodium and Lithium response/Resistance Behavior Using the Yeast Saccharomyces Cerevisiae As a Model Organism”. Journal of Faculty of Pharmacy of Istanbul University, c. 45, sy. 2, 2016, ss. 165-79.
Vancouver Carola Hunte 4, Z. Petek Çakar1,2 ŞHT1A1. Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University. 2016;45(2):165-79.