CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY

Year 2012, Volume: 1 Issue: 42(1), 13 - 22, 10.01.2014

E. Türköz Acar D. Özer Ünal M. Koç E. Türköz Acar1, D. Özer Ünal2,*, M. E. Türköz Acar1, D. Özer Ünal2,*, M. Koç3

Abstract

In this study, a simple, rapid and accurate capillary electrophoresis method has been developed for the determination of lactulose and mannitol in urine for estimating the intestinal permeability. The urine samples were prepared to analyze by using solid phase extraction. For analysis a background electrolyte (BGE) consisted of 6 mmol/L sorbic acid, 1.25mmol/L hexadecyltrimeth ylammoniumbromide (HDTMA), 50 mmol/L LiOH (pH 12.5) was used. Separation was performed at -5 kV over 20 min within the fused capillary column (45 cm, 50 µm I.D.) at 20 °C. The method developed was applied a series of urine sample to measure the lactulose/mannitol concentration rate.

Keywords

Lactulose, Mannitol, Determination, Permeability, Capillary electrophoresis

CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY

Abstract

Bu çalışmada intestinal geçirgenliğin incelenmesi amacıyla idrarda
laktuloz ve mannitol tayini için basit, hızlı ve doğru bir kapiler elektroforez
yöntemi geliştirilmiştir. İdrar örnekleri katı faz özütleme yöntemi ile analize
hazırlanmıştır. Analizler için 6 mmol/L sorbik asit, 1.25 mmol/L hekzadesil
trimetil amonyum bromür (HDTMA), 50 mmol/ L LiOH içeren pH sı 12.5
e karşılık gelen destek elektrolit kullanılmıştır. Ayrım 45 cm 50 µm iç çaplı
fused silika kolonda 20 °C sıcaklıkta -5 kV ayırma potansiyeli kullanılarak
20 dk içerisinde gerçekleştirilmiştir. Geliştirilen yöntem laktuloz / mannitol
derişim oranlarını ölçmek için bir seri idrar numunesine uygulanmıştır.

Keywords

Laktuloz, Mannitol, Tayin, Geçirgenlik, Kapiler elektroforez

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