CYTOTOXIC EFFECTS OF TRIAZOLE FUNGUCIDES

Year 2012, Volume: 1 Issue: 42(1), 23 - 31, 10.01.2014

E.N Bülbül G. Özhan E.n. Bülbül, G. Özhan E.n. Bülbül, G. Özhan

Abstract

CYTOTOXIC EFFECTS OF TRIAZOLE FUNGUCIDES

Abstract

Fungicides are at high risk exposure compounds both pre and post harvest
time due to their usage during storage. Fungucides have various benefits
on agricultural struggle. Apart from that, studies showed that fungucides
also have mutagenic, carcinogenic, chronic toxic effects on nervous system, immune system, hormonal and reproductive system. Triazole group
fungicides, prochloraz, tebuconazole, triadimenole and triadimefone, are
commonly used in the world. In the study, it is aimed to emphasize about
their importance on human and environmental health. And, we evaluated
their potential cytotoxic effect on HeLa cells by using MTT and LDH
tests. Before the tests application, the parameters like cell counts, incubation times, pesticide concentrations and test circumstances were examined
for test optimization. As a result, for MTT cytotoxicity test IC50 values of
prochloraz, tebuconazole, triadimefone and triadimenole were recorded in
order as 0.4, >1, 0.24 and 0.37 mg/ml. As well for LDH test these values
were identified in order as 0.065, 0.985, 0.052 and 0.042 mg/ml. MTT test,
used to identify hazards on mitochondrial activity and respiratory chain,
showed that cytotoxic effect of prochloraz, triadimefone and triadimenole
was dose dependently increased. While tebuconazole showed cytotoxic
effect on concentration range studied, it was seen that IC50 values for LDH
test which is used to identify membrane damage were lower than IC50 values compared with MTT test. The obtained results would draw attention to
expand researches on toxic effects of triazole fungicides which are identified
as cytotoxic for human and environment health. Also, it is believed to take
necessary precautions for usage of these compounds

Keywords

Triazole fungicides, MTT test, LDH test, Cytotoxicity

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