THE EVALUATION OF TURKISH AUTUMN FLOWERING COLCHICUM SPECIES

Yıl 1998, Cilt: 32 , 1 - 8, 09.09.2015

N. Sütlüpınar

Öz

65 Colchicum species grow all over the World and 25 of them grow in
Turkey.
In this work, Turkish Colchicum species with respect to the tropolone alkaloids
that have gained importance in pharmaceutical industry have been evaluated.
First of all, the preparative TLC separation and spectrophotometric determination
of colchicine and demecolcine were accomplished. Species with low
alkaloid content and with small seeds and corms which have not economic value
were eliminated and 10 Colchicum species were investigated with respect to
their total tropolonic alkaloid content using a direct spectrophotometric method.
HPLC was also used to quantitate the amount of colchicine, colchicoside and
demecolcine. The results obtained were compared with those of C.autumnale
cited in the Pharmacopeiae.
As a result of our investigations, alkaloid content of C.baytopiol-urn, bivonae,
bol-nmuelleri, cilicicum, kotschyi and speciosum species were found as
rich as or even richer than C.autumnale.

Key words: Colchicum, Colchicine, Colchicoside, Demecolcine, Tropo-
Ionic Alkaloids.

Anahtar Kelimeler

-

THE EVALUATION OF TURKISH AUTUMN FLOWERING COLCHICUM SPECIES

Öz

65 Colchicum species grow all over the World and 25 of them grow in
Turkey.
In this work, Turkish Colchicum species with respect to the tropolone alkaloids that have gained importance in pharmaceutical industry have been evaluated. First of all, the preparative TLC separation and spectrophotometric determination of colchicine and demecolcine were accomplished. Species with low
alkaloid content and with small seeds and corms which have not economic value
were eliminated and 10 Colchicum species were investigated with respect to
their total tropolonic alkaloid content using a direct spectrophotometric method.
HPLC was also used to quantitate the amount of colchicine, colchicoside and
demecolcine. The results obtained were compared with those of C.autumnale
cited in the Pharmacopeiae.
As a result of our investigations, alkaloid content of C.baytopiol-urn, bivonae, bol-nmuelleri, cilicicum, kotschyi and speciosum species were found as
rich as or even richer than C.autumnale.

Anahtar Kelimeler

Colchicum, Colchicine, Colchicoside, Demecolcine, TropoI lonic Alkaloids

Kaynakça

• 1. Willis, J.C., A Dictionary of the Flowering Plants & Ferns 265, University Press, Cambridge (1966).
• 2. Brickell, C.D. in Davis, P.H.: Flora of Turkey & the East Aegean Islands, Vo1.8, p. 329-351, Edinburgh University Press, Edinburgh (1984).
• 3. Siitliiplnar, N., Tiirkiye'de Yeti~en ve SonbahafPa Ciqek Agan Colchicum Tiirlerinin Kol~isin ve Demekolsin Yoniinden Arqhnlrnasl, TUBITAK, TBAG-399, Istanbul (I98 1).
• 4. Siitliipmar, N., Tiirkiye'nin Sonbahar Colchicum Tiirlerinin Demekolsin, Kol~ikozit ve Kolaisin Yoniinden Degerlendirilmesi, 1.U. Eczaclllk Fakiiltesi Do~entlik tezi, Istanbul (1985).
• 5. Siitliipmar, N., Husek, A., PotSsilovi, H., D'vorickovi,~., Hanus, V., Sedmera, P., Siminek, V. Planta Medica, 54,243-245 (1988).
• 6. Husek, A., Siitliiplnar, N., PotCsilovi, H., DvorBckovB, S., Hanus, V., Sedmera, P., Malon, P., Siminek, V. Phytockemistry, 28,3217-3219 (1989).
• 7. Husek, A., Siitliipmar, N., ~edhera, P., Siminek, V. Heterocycles, 28,79-82 (1989).
• 8. Siminek, V., Husek, A., Vilka, I., Siitliipmar, N. Herba Hungarica, 29,64-68 (1990)