Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera Fisch. & Ave-Lall.

Year 2021, , 365 - 371, 29.12.2021

Nurdan Yazıcı Bektaş , Burak Barut , Emel Mataracı Kara , Yeter Yeşil

https://doi.org/10.26650/IstanbulJPharm.2021.1016208

Abstract

Background and Aims: This study aims to determine total phenolic, total flavonoid contents and in vitro biological activities of methanol (CEP-1), n-butanol (CEP-2), water (CEP-3), n-hexane (CEP-4) extracts obtained from Cephalaria procera. Methods: The total phenolic and flavonoid content analysis, in vitro DPPH radical scavenging activities, cholinesterase, and tyrosinase inhibitory properties of the extracts were evaluated using spectrophotometric assays. DNA-damage and DNAdamage protective effects of the extracts were examined using agarose gel electrophoresis method. Antimicrobial activities of the extracts were determined by microdilution method. Results: CEP-3 had the best total phenolic content (79.64±1.11 mg GAE/g dry weight), and CEP-1 had the highest total flavonoid content (15.33±0.27 mg QEE/g dry weight) among tested extracts. CEP-1 showed the highest radical scavenging activity with 83.21±3.20 μg/mL of IC50 value. CEP-3 exerted the highest AChE and BuChE inhibitory action with 134.63±4.49 μg/mL and 62.76±0.63 μg/mL of IC50 values, respectively. CEP-3 showed significant tyrosinase inhibitory action with 51.95±0.35 μg/mL IC50 value compared to kojic acid (58.26±0.25 μg/mL). CEP-1 and CEP-3 were tested, and the both extracts did not damage supercoiled DNA at studied concentrations. Incidentally, results indicated that CEP-1 and CEP-3 protected supercoiled DNA against Fenton’s reagents. CEP-4 exhibited the highest antimicrobial activity on C. tropicalis with the MIC value of 156.2 μg/mL. Conclusion: The results showed that crude and subextracts of C. procera exerted several moderate activities on tested systems. It suggested that the species might be a promising medicinal plant for the treatment or prevention of several diseases associated with skin damage and wounds.

Keywords

Antioxidant, antimicrobial, anticholinesterase, Cephalaria procera, DNA protective, tyrosinase

References

• Akar, Z., Küçük, M., Doğan, H. (2017). A new colorimetric DPPH* scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 643-647. http://dx.doi.org/10.1080/14756366.2017.1284068
• AlFaris, N.A., AlTamimi, J.Z., AlGhamidi, F.A., Albaridi, N.A., Alzaheb, R.A., Aljabryn, D.H., AlMousa, L.A. (2021). Total phenolic content in ripe date fruits (Phoenix dactylifera L.): A systematic review and meta-analysis. Saudi Journal of Biological Sciences. https://doi.org/10.1016/j.sjbs.2021.03.033
• Barut, B., Barut, E.N., Engin, S., Özel, A., Sezen, F.S. (2019). Investigation of the antioxidant, α-glucosidase inhibitory, anti-inflammatory, and DNA protective properties of Vaccinium arctostaphylos L. Turkish Journal Of Pharmaceutical Sciences, 16, 175-183. DOI: 10.4274/tjps.galenos.2018.28247
• Barut, E.N., Barut, B., Engin, S., Yıldırım, S., Yaşar, A., Turkis, S., Özel, A., Sezen, F.S. (2017). Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Turkish Journal of Biochemistry, 42, 493-502
• Barut, B., Şöhretoğlu, D. (2020). Total phenolic content, cyclooxygenases, α-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits. Journal of Research in Pharmacy. 24(5); 623-631. https://doi.org/10.35333/jrp.2020.217
• Boke Sarıkahya, N., Goren A.C., Sumer Okkali, G., Kirmizigul, S. (2021). Saponins from twenty-two Cephalaria species. Records of Natural Products, 15(6); 537-546. http://doi.org/10.25135/rnp.241.21.02.1985
• Böke Sarıkahya, N., Kırmızıgül, S. (2010). Antimicrobial triterpenoid glycosides from Cephalaria scoparia. Journal of Natural Products, 73, 825–830. Doi: 10.1021/np900724u
• Böke Sarıkahya, N., Pekmez, M., Arda, N., Kayce, P., Karabay Yavaşoğlu, N.Ü., Kırmızıgül, S. (2011). Isolation and characterization of biologically active glycosides from endemic Cephalaria species in Anatolia. Phytochemistry Letters, 4, 415-420. doi:10.1016/j.phytol.2011.05.006
• Celenk, V.U., Boke Sarikahya, N., Kirmizigul, S. (2020). Isolation and structural studies on saponins from three Cephalaria species from Anatolia. Chemistry of Natural Compounds, 56(1). DOI 10.1007/s10600-020-02980-w
• Chrzaszcz, M., Krzeminska, B., Celinski, R., & Szewczyk, K. (2021). Phenolic composition and antioxidant activity of plants belonging to the Cephalaria (Caprifoliaceae) genus. Plants, 10; 952. https://doi.org/10.3390/plants10050952
• Clinical and Laboratory Standards Institute. (2020). Performance standards for antimicrobial susceptibility testing,30th Edition in, Clinical and Laboratory Standards Institute; M100. Wayne, PA, USA.
• Clinical and Laboratory Standards Institute. (1997). Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard–Second Edition; M27-A2. Wayne, PA, USA.
• Faydaoğlu, E., & Sürücüoğlu, M. S. (2011). Geçmişten günümüze tıbbi ve aromatik bitkilerin kullanılması ve ekonomik önemi. Journal of Forestry Faculty, 11(1), 52–57.
• Godjevac, D., Vajs, V., Menkovic, N., Tesevic, V., Janackovic, P., Milosavijevic, S. (2004). Flavonoids from flowers of Cephalaria pastricensis and their antiradical activity. Journal of the Serbian Chemical Society, 69 (11) 883–886.
• Göktürk, R.S. & Sümbül, H. (2014). A taxonomic revision of the genus Cephalaria (Caprifoliaceae) in Turkey, Turkish Journal of Botany, 38; 927-968. doi:10.3906/bot-1310-6.
• Kahraman, C., Baysal, I., Çankaya, I., Goger, F., Kirimer, N., & Akdemir, Z.S. (2019). Acetylcholinesterase inhibitory activities and LC-MS analysis of the antioxidant Ferula caspica M. Bieb. and F. halophila Pesmen extracts. DOI: 10.12991/jrp.2019.161
• Liang, N., & Kitts, D.D. (2014). Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules, 19(11), 19180-19208. https://doi.org/10.3390/molecules191119180
• Morais, D.V.D., Costa, M.A.P.D.C., Santa Bárbara, M. F., Silva, F.D.L., Moreira, M.M., Delerue-Mato, C., Carvalho, C.A.L.D. (2018). Antioxidant, photoprotective and inhibitory activity of tyrosinase in extracts of Dalbergia ecastaphyllum. PloS one, 13(11), e0207510. https://doi.org/10.1371/journal.pone.0207510
• Mustafayeva, K., Mahiou-Leddet, V., Suleymanow, T., Kerimov, Y., Ollivier, E., Elias, R. (2011). Chemical constituents from the roots of Cephalaria kotschyi. Chemistry of Natural Compounds, 47(5).
• Newman, D. J., & Cragg, G.M. (2020). Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products, 83(3).
• Özgen, U., Kaya, Y., Houghton, P. (2012). Folk Medicine in the villages of Ilıca District (Erzurum, Turkey). Turkish Journal of Biology, 36; 93-106. doi:10.3906/biy-1009-124
• Pasi, S., Aligiannis, N., Skaltsounis, A.L., Chinou, I.B. (2002). A new lignan glycoside and other constituents from Cephalaria ambrosioides. Natural Product Letters, 16(6), 365–370. Doi: 10.1080/10575630290030720
• Sarikahya, N.B., Goren, A.C., & Kirmizigul, S. (2019). Simultaneous determination of several flavonoids and phenolic compounds in nineteen different Cephalaria species by HPLC-MS/MS. Journal of pharmaceutical and biomedical analysis, 173, 120-125. doi: 10.1016/j.jpba.2019.05.019.
• Sarikahya, N.B., Ucar, E.O., Kayce, P., Gokturk, R.S., Sumbul, H., Arda, N., Kirmizigul, S. (2015). Fatty acid composition and antioxidant potential of ten Cephalaria species. Records of Natural Products, 9, 116-123.
• Sarıkahya, N.B., & Kırmızıgül, S. (2012). Antimicrobially active hederagenin glycosides from Cephalaria elmaliensis. Planta medica, 78(08), 828-833. DOI: 10.1055/s-0031-1298415.
• Sun, L., Guo, Y., Zhang, Y., & Zhuang, Y. (2017) Antioxidant and anti-tyrosinase activities of phenolic extracts from rape bee pollen and inhibitory melanogenesis by cAMP/MITF/TYR pathway in B16 mouse melanoma cells. Frontiers in Pharmacology. 8(104). doi: 10.3389/fphar.2017.00104
• Şöhretoğlu, D., Barut ,B., Sari, S., Özel, A., Arroo, R. (2020). In vitro and in silico assessment of DNA interaction, topoisomerase I and II inhibition properties of chrysosplenetin. International Journal of Biological Macromolecules, 163; 1053-1059. Doi: 10.1016/j.ijbiomac.2020.07.049
• Şöhretoğlu, D., Sari, S., Barut, B., & Özel, A. (2018). Tyrosinase inhibition by some flavonoids: Inhibitory activity, mechanism by in vitro and in silico studies. Bioorganic chemistry, 81, 168-174. doi: 10.1016/j.bioorg.2018.08.020.
• Top, H., Boke Sarikahya, N., Nalbantsoy, A., Kirmizigul, S. (2017). Immunomodulatory, hemolytic properties and cytotoxic activity potent of triterpenoid saponins from Cephalaria balansae. Phytochemistry, 137; 139-147.
• Tuğrak, M., Gül, H. İ., & Gülçin, İ. (2020). Acetylcholinesterase inhibitory potencies of new pyrazoline derivatives. Journal of Research in Pharmacy, 24(4), 464-471. DOI : 10.35333/jrp.2020.194
• Wang, Y., Hao, M. M., Sun, Y., Wang, L. F., Wang, H., Zhang, Y. J., Li H. Y, Zhuang PW & Yang, Z. (2018). Synergistic promotion on tyrosinase inhibition by antioxidants. Molecules, 23(1), 106. https://doi.org/10.3390/molecules23010106