The effects of chard extract against streptozotocin-induced erectile dysfunction in rats

Yıl 2024, , 12 - 19, 30.04.2024

Mustafa Aydın , Özlem Saçan , Levent Kabasakal , Şule Çetinel , Mustafa Kadıhasanoglu , Muammer Kendirci , Refiye Yanardağ , Göksel Şener

https://doi.org/10.26650/IstanbulJPharm.2024.1070319

Öz

Background and Aims: To analyze the potential therapeutic effects of chard against streptozotocin (STZ) -induced erectile dysfunction (ED) and oxidative damage in the corpus cavernousum in rats.

Materials and Methods: In this study, Sprague-Dawley rats (250-300g) were allocated into groups as follows: control, diabetic, diabetic + chard, and diabetic + insulin. In order to induce diabetes, rats were given 65 mg/kg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 g/kg for 45 days beginning on 15th days. Sixty days after STZ injection, intracavernosal pressure (ICP) was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine (ADMA)levels, and lactate dehydrogenase (LDH) activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence (CL), malondialdehyde and glutathione and along with histological analysis.

Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values.

Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes-induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties

Anahtar Kelimeler

Chard extract, diabetes mellitus, erectile dysfunction, oxidative damage

Destekleyen Kurum

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Proje Numarası

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Kaynakça

• Ahn, G.J., Sohn, Y.S., Kang, K.K., Ahn, B.O., Kwon, J.W., Kang, S.K.,& Wang, W.S. (2005). The effect of PDE5 inhibition on the erectile function in streptozotocin-induced diabetic rats. International Journal of Impotence Research, 17, 134-141. https://doi.org/ 10.1038/sj.yir.3901295. google scholar
• Beuge, J.A., & Aust, S.D. (1978). Microsomal lipid peroxida-tion. Methods in Enzymology, 52, 302-311. https://doi.org/ 10.1016/s0076-6879(78)52032-6. google scholar
• Beutler, E., &Gelbart, T. (1986). Improved assay of the enzymes of glutathione synthesis: Gamma-glutamylcysteine synthetase and glutathione synthetase. Clinica Chimica Acta, 158, 115-123. https://doi.org/ 10.1016/0009-8981(86)90122-1. google scholar
• Bivalacqua, T.J., Champion, H.C., Hellstrom, W.J., & Kad-owitz, P.J. (2000). Pharmacotherapy for erectile dysfunction. Trends Pharmacological Sciences, 21, 484-489. https://doi.org/ 10.1016/s0165-614(00)01587-x. google scholar
• Bivalacqua, T.J., Usta, M.F., Champion, H.C., Adams, D., Namara, D.B., Abdel-Mageed, A.B., &Hellstrom, W.J.G. (2003). Gene transfer of endothelial nitric oxide synthase partially restores ni-tric oxide synthesis and erectile function in streptozotocin dia-betic rats. Journal of Urology, 169, 1911-1917. https://doi.org/10.1097/01.ju.0000051881.14239.4a. google scholar
• Bolkent, S., Yanardağ, R., Tabakoğlu-Oğuz, A., &Ozsoy-Saçan, O. (2000). Effects of chard (Beta vulgaris L. var. cicla) extract on pancreatic B cells in streptozotocin-diabetic rats: a morphological and biochemical study.Journal of Ethnopharmacology, 73, 251259. https://doi.org/ 10.1016/s0378-8741(00)00328-7. google scholar
• Bozkalfa, M., Eşiyok, D., &Kaygisiz-Aşçioğul, T. (2016). Diver-sity pattern among agromorphological traits of the Swiss chard (Beta vulgaris L. subsp. vulgaris) genetic resources of Turkey. Turkish Journal of Agriculture and Forestry, 40, 684-695. https://doi.org/10.3906/tar-1512-80. google scholar
• De Young, L., Yu, D., Freeman, D., & Brock, G.B. (2003). Effect of PDE5 inhibition combined with free oxygen radical scav-enger therapy on erectile function in a diabetic animal model. International Journal of Impotence Research, 15, 347-354. https://doi.org/ 10.1038/sj.yir.3901026. google scholar
• De Young, L., Yu, D., Bateman, R.M., & Brock, G.B. (2004). Ox-idative stress and antioxidant therapy: their impact in diabetes-associated erectile dysfunction. Journal of Andrology, 25, 830-836. https://doi.org/ 10.1002/j.1939-4640.tb02862.x. google scholar
• Ertik,O.,Sacan,O., Kabasakal, L.,Sener,G.,&Yanardag, R. (2021). Protective effect of chard extract on glycoprotein compounds and advanced oxidation protein product levels in diabetic rat livers. Experimed,11, 27-32. https://doi.org/ 10.26650./exper-imed.2021.879204. google scholar
• Gezginci-Oktayoglu, S., Sacan, O., Bolkent, S., Ipci, Y., Kabasakal, L., Sener, G., &Yanardag, R. (2014). Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increas-ing GLUT2 through Akt2 and antioxidant defense in the liver of rats. Acta Histochemica, 116, 32-39. https://doi.org/ 10.1016/j.acthis.2013.04.016. google scholar
• Grover, J.K., Yadav, S., & Vats, V. (2002). Medicinal plants of Indiana with anti-diabetic potential. Journal of Ethnopharmacology, 81, 81-100. https://doi.org/ 10.1016/s0378-8741(02)0059-4. google scholar
• Haklar, U., Yüksel, M., Velioglu, A., Turkmen, M., Haklar, G., &Yalçın, A.S. (2002). Oxygen radicals and nitric oxide lev-els in chondral or meniscal lesions or both. Clinical Or-thopaedics and Related Research, 403, 135-142. https://doi.org/ 10.1097/00003086-200210000-00021. google scholar
• Hunt, J.V., Smith, C.C., & Wolff, S.P. (1999). Autooxidative gly-cosylation and possible involvement of peroxides and free rad-icals in LDL modification by glucose. Diabetes, 3, 1420-1424. https://doi.org/ 10.2337/diab.39.11.1420. google scholar
• Junod, A., Lambert, A.E., Stauffacher, W., & Renold, A.E. (1969). Diabetogenic action of streptozotocin: relationship of dose to metabolic response. Journal of Clinical Investigation, 48, 21292139. https://doi.org/ 10.1172/JCI106180. google scholar
• Kesavulu, M.M., Rao, B.K., Giri, R., Vyaya, J., Subramanyam, G., &Apparao, C. (2001). Lipid peroxidation and antioxidant en-zyme status in Type 2 diabetics with coronary heart disease. Di-abetes Research and Clinical Practice, 53, 33-39. https://doi.org/ 10.1016/S0168-8227(01)00238-8. google scholar
• Kielstein, J.T., Bode-Böger, S.M., Frölich, J.C., Ritz, E., Haller, H., &Fliser, D. (2003).Asymmetric dimethylarginine, blood pressure, and renal perfusion in elderly subjects. Circulation, 107, 18911895. https://doi.org/ 10.1161/01.CIR.0000060496.23144.A7. google scholar
• Neef, H., Declercq, P., & Laekeman, G. (1995). Hypoglycemic activity of selected European plants. Phytotherapy Research, 9, 45-48. google scholar
• Ozel, A.B., Cilingir-Kaya, O.T., Sener, G., Ozbeyli, D., Sen, A., Sacan, O.,Yanardag, R., & Yarat, A. (2021). Investigation of possible neuroprotective effects of some plant extracts on brain in bile duct ligated rats. Journal of Food Biochemistry, 45, e 13835.https://doi.org/10.1111/jfbc.13835. google scholar
• Ozsoy-Sacan, O., Karabulut-Bulan, O., Bolkent, S., Yanardag, R., &Ozgey, Y. (2004). Effects of chard (Beta vulgaris var. cicla) on the liver of the diabetic rats: a morphological and biochemi-cal study. Bioscience and Biotechnology Biochemistry, 68, 16401648. https://doi.org/10.1271/bbb.68.1640. google scholar
• Oztay, F., Sacan, O., Kayalar, O., Bolkent, S., Ipci, Y., Kabasakal, L.,& Yanardag R. (2015). Chard (Beta vulgaris var. cicla) extract improved hyperglycemia-induced oxidative stress and surfactant-associated protein alterations in rat lungs. Pharmaceutical Biol-ogy, 53, 1639-1646. https://doi.org/10.3109/13880209. google scholar
• Rendell, M.S., Rajfer, J., Wicker, P.A., & Smith, M.D. (1999). Silde-nafil for treatment of erectile dysfunction in men with dia-betes: a randomized controlled trial. Sildenafil Diabetes Study Group. Journal of American Medical Association, 281, 421-426. https://doi.org/10.1001/jama.281.5.421. google scholar
• Ryu, J.K., Kim, D.J., Lee, T., Kang, Y.S., Yoon, S.M., & Suh, J.K. (2003). The role of free radical in the patho-genesis of impotence in streptozotocin-induced diabetic rats. Yonsei Medical Journal, 44, 236-241. https://doi.org/10.3349/ymj.2003.44.2.236. google scholar
• Sacan, O., & Yanardag, R. (2010). Antioxidant and anti-acetylcholinesterase activities of chard (Beta vulgaris L. var. cicla). Food and Chemical Toxicology, 48, 1275-1280. https://doi.org/10.1016/j.fct.2010.02.022. google scholar
• Sacan, O., Ertik, O., Ipci, Y., Kabasakal, L., Sener, G., & Yanardag, R. (2018). Protective effect of chard extract on glycoprotein com-pounds and enzyme activities in streptozotocin-induced hyper-glycemic rat lungs. Bulgarian Chemical Communications, 50, 119-123. google scholar
• Saenz de Tejada, I., Goldstein, I., Azadzoi, K., Krane, R.J., & Cohen, R.A. (1989). Impaired neurogenic and endothelium-mediated relaxation of penile smooth muscle from diabetic men with impotence. New England Journal of Medicine, 320, 1025-1030. https://doi.org/10.1056/NEJM198904203201601. Retrived from http://bcc.bas.bg/BCC_Volumes/Volume_50_Number_1_2018/BCC 50-1-2018-119-123-4450. google scholar
• Seftel, A.D., Vaziri, N.D., Ni, Z., Razmjouei, K., Fogarty, J., Ham-pel, N., &Haas, C. (1997). Advanced glycation end products in human penis: elevation in diabetic tissue, site of deposition, and possible effect through iNOS or eNOS. Urology, 50, 1016-1026. https://doi.org/10.1016/S0090-4295(97)00512-8. google scholar
• Sener, G., Saçan, O., Yanardağ, R., &Ayanoğlu-Dülger, G. (2002). Effects of chard (Beta vulgaris L. var. cicla) extract on oxidative injury in the aorta and heart of streptozotocin-diabetic rats. Journal of Medicinal Food, 5, 37-42. https://doi.org/10.1089/109662002753723205. google scholar
• Taylor, A.A. (2001). Pathophysiology of hypertension and endothe-lial dysfunction in patients with diabetes mellitus. Endocrinol-ogy and Metabolism Clinics of North America, 30, 983-997. https://doi.org/10.1016/s0889-8529(05)70223-1. google scholar
• Tunali, T., Yarat, A., Yanardağ, R., Ozçelik, F., Ozsoy, O., Er-genekon, G., & Emekli, N. (1998). The effect of chard (Beta vulgaris L. var cicla) on the skin of streptozotocin-induced diabetic rats. Pharmazie, 53, 638-640. Retrieved from https://pubmed.ncbi.nlm.nih.gov/9770212. google scholar
• Tunali, S., Cimen, E.S., & Yanardag, R. (2020). The effects of chard on brain damage in valproic acid-induced toxicity. Journal of Food Biochemistry, 44, e 13382. https://doi.org/ 10.1111/jfbc.13382. google scholar
• Ustundag, U.V., Tunali, S., Alev B., İpekci, H., Emekli-Alturfan, E., Tunali,T,.Yanardag, R.,&Yarat, A.(2016). Effects of chard (Beta vulgaris L. var. cicla) on cardiac damage in valproic acid-induced toxicity. Journal of Food Biochemistry, 40, 132-139.https://doi.org/10.1111/jfbc.12202. google scholar
• Veresh, Z., Racz, A., Lotz, G., & Koller, A. (2008). ADMA impairs nitric oxide-mediated arteriolar function due to increased superoxide production by angiotensin II-NAD(P)H oxidase pathway. Hypertension, 52, 960-966 https://doi.org/10.1161/HYPERTENSIONAHA.108.116731. google scholar
• Way, K.J., & Reid, J.J. (1999). The effects of diabetes on nitric oxide-mediated responses in rat corpus cavernosum. European Jour-nal of Pharmacology, 376, 73-82. https://doi.org/10.1016/s0014-2999(99)00347-7. google scholar
• Yanardağ, R., & Çolak, H. (1998). Effect of chard (Beta vulgaris L. var cicla) on blood glucose levels in normal and alloxan-induced diabetic rabbits. Pharmacy and Pharmacology Communications, 4, 309-311. https://doi.org/10.1111/j.2042-7158-1998.tb00702.x. google scholar
• Yanardağ, R., Bolkent, Ş., Özsoy-Saçan, Ö., & Karabulut-Bulan, Ö. (2002). The effect of chard (Beta vulgaris L. var ci-cla) extract on the kidney tissue, serum urea and creatinine levels of diabetic rats. Phytotherapy Research, 16, 758-761. https://doi.org/10.1002/ptr.1041. google scholar