Physiological, oxidative, and antioxidative responses of Quercus vulcanica Boiss. and Quercus aucheri Jaub. & Spach. under drought stress conditions

Yıl 2024, , 69 - 79, 30.04.2024

Hakan Özden , Gulriz Baycu

https://doi.org/10.26650/IstanbulJPharm.2024.1404110

Öz

Background and Aims: Drought stress is one of the most common global factors of abiotic stress affecting plant growth and productivity world-wide. The present study aims to assess the effects of 1, 2, and 4 weeks of drought stress on the two endemic tree species, Quercus vulcanica and Q. aucheri

Methods: After applying drought stress conditions, the study determines the physiological parameters, markers of oxidative damage, and levels of antioxidant enzymes in the leaves and stems of 6-month-old oak seedlings.

Results: The dry and fresh weights were observed to decrease by at least 11.44%, as well as the chlorophyll levels by at least 14%, in both the Q. vulcanica and Q. aucheri that were subjected to 1, 2, and 4 weeks of drought stress. The carotenoid, proline, and anthocyanin levels also increased in the leaves of both Quercus species; however, the amount of ascorbic acid, reduced glutathione (GSH), and total soluble protein decreased in the leaves and stems of both Quercus species. As oxidative stress markers, the levels of hydrogen peroxide (H2O2) and lipid peroxidation were seen to elevate by at least 1.21 fold under drought stress conditions. This revealed some of the alterations in the activities of antioxidant enzymes in the leaves and stems of both Q. aucheri and Q. vulcanica.

Conclusion: In conclusion, this study revealed increasing drought stress to have a substantial impact on the physiological parameters and oxidative and antioxidant responses in Q. vulcanica and Q. aucheri. This will contribute to understanding how oak species respond to drought stress and their adaptation strategies.

Anahtar Kelimeler

Q. vulcanica, Q. aucheri, drought stress, oxidative stress, antioxidant enzyme system

Destekleyen Kurum

Scientific Research Projects Coordination Unit of Istanbul University

Proje Numarası

T-756/13092005

Teşekkür

The authors would like thank to Prof. Mesut Kirmaci for the helping to collect the Quercus seeds and Prof. Osman Erol and Assist. Prof. Erdal Uzen for helping to transfer the seeds

Kaynakça

• Aebi, H. (1984). Catalase in vitro. In Methods in Enzymology (Vol. 105, pp. 121-126): Academic Press. google scholar
• Alonso, R., Elvira, S., Castillo, F., & Gimeno, B. (2001). Interactive effects of ozone and drought stress on pigments and activities of antioxidative enzymes in Pinus halepensis. Plant, Cell & Envi-ronment, 24(9), 905-916. google scholar
• Bates, L. S., Waldren, R. a., & Teare, I. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207. google scholar
• Baycu, G., Tolunay, D., Özden, H., & Günebakan, S. (2006). Eco-physiological and seasonal variations in Cd, Pb, Zn, and Ni con-centrations in the leaves of urban deciduous trees in Istanbul. Environmental Pollution, 143(3), 545-554. google scholar
• Beyer Jr, W. F., & Fridovich, I. (1987). Assaying for superoxide dis-mutase activity: some large consequences of minor changes in conditions. Analytical Biochemistry, 161(2), 559-566. google scholar
• Bradford, M. M. (1976). A rapid and sensitive method for the quanti-tation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254. google scholar
• Burton, G. W., & Ingold, K. (1984). p-Carotene: an unusual type of lipid antioxidant. Science, 224(4649), 569-573. google scholar
• Cakmak, I. (1994). Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium-and potassium-deficient leaves, but not in phosphorus-deficient leaves. Journal of Experimental Botany, 45(9), 1259-1266. google scholar
• Cotrozzi, L., Pellegrini, E., Guidi, L., Landi, M., Lorenzini, G., Massai, R., . . . Vernieri, P. (2017). Losing the warning signal: drought compromises the cross-talk of signaling molecules in Quercus ilex exposed to ozone. Frontiers in Plant Science, 8, 1020. google scholar
• Cotrozzi, L., Remorini, D., Pellegrini, E., Guidi, L., Lorenzini, G.,Massai, R., . . . Landi, M. (2017). Cross-talk between physio-logical and metabolic adjustments adopted by Quercus cerris to mitigate the effects of severe drought and realistic future ozone concentrations. Forests, 8(5), 148. google scholar
• Cotrozzi, L., Remorini, D., Pellegrini, E., Landi, M., Massai, R., Nali, C., . . . Lorenzini, G. (2016). Variations in physiological and biochemical traits of oak seedlings grown under drought and ozone stress. Physiologia Plantarum, 157(1), 69-84. google scholar
• Creissen, C. P., Boardbent, P., Kular, B., Reynolds, H., Welburn, A. R., & Mullineaux, P. M. (1994). Manupilation of glutathione re-ductase in transgenic plants: implications for plants response to environmental stress. Proc. R. Soc. Edinburg, 102, 167-175. google scholar
• Çinar, I. (2004). Carotenoid pigment loss of freeze-dried plant sam-ples under different storage conditions. LWT-Food Science and Technology, 37(3), 363-367. google scholar
• Dhanda, S., & Sethi, G. (1998). Inheritance of excised-leaf water loss and relative water content in bread wheat (Triticum aestivum). Euphytica, 104, 39-47. google scholar
• Echevama-Zomeno, S., Ariza, D., Jorge, I., Lenz, C., Del Campo, A., Jom'n, J. V., & Navarro, R. M. (2009). Changes in the protein profile of Quercus ilex leaves in response to drought stress and recovery. Journal of Plant Physiology, 166(3), 233-245. google scholar
• Egert, M., & Tevini, M. (2002). Influence of drought on some physi-ological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environmental and Experimental Botany, 48(1), 43-49. google scholar
• Foyer, C. H., & Halliwell, B. (1976). The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta, 133, 21-25. google scholar
• Galle, A., & Feller, U. (2007). Changes of photosynthetic traits in beech saplings (Fagus sylvatica) under severe drought stress and during recovery. Physiologia Plantarum, 131(3), 412-421. google scholar
• Ghanbary, E., Tabari Kouchaksaraei, M., Zarafshar, M., Bader, K. F. M., Mirabolfathy, M., & Ziaei, M. (2020). Differential physiologi-cal and biochemical responses of Quercus infectoria and Q. libani to drought and charcoal disease. Physiologia Plantarum, 168(4), 876-892. google scholar
• Gossett, D. R., Millhollon, E. P., & Lucas, M. C. (1994). Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop science, 34(3), 706-714. google scholar
• Halliwell, B., & Gutteridge, J. M. (2015). Free Radicals in Biology and Medicine: Oxford university press. google scholar
• Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxida-tion. Archives of Biochemistry and Biophysics, 125(1), 189-198. google scholar
• Horwitz, W. (1970). Official methods of analysis of the Association of Official Analytical Chemists. Washington, DC: The Association Washington, DC. google scholar
• Karpinski, S., Reynolds, H., Karpinska, B., Wingsle, G., Creissen, G., & Mullineaux, P. (1999). Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science, 284(5414), 654-657. google scholar
• Khanna-Chopra, R., & Selote, D. S. (2007). Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than-susceptible wheat cultivar under field conditions. Environ-mental and Experimental Botany, 60(2), 276-283. google scholar
• Kocheva, K. V., & Georgiev, G. I. (2008). Changes in foliar proline concentration of osmotically stressed barley. Zeitschrift für Natur-forschung C, 63(1-2), 101-104. google scholar
• Landi, M., Cotrozzi, L., Pellegrini, E., Remorini, D., Tonelli, M., Trivellini, A., . . . Vernieri, P. (2019). When “thirsty” means “less able to activate the signalling wave trigged by a pulse of ozone”: A case of study in two Mediterranean deciduous oak species with different drought sensitivity. Science of the Total Environment, 657, 379-390. google scholar
• Lichtenthaler, H. K. (1996). Vegetation stress: an introduction to the stress concept in plants. Journal of Plant Physiology, 148(1-2), 4-14. google scholar
• Lichtenthaler, H. K., & Wellburn, A. R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. In: Portland Press Ltd. google scholar
• Mancinelli, A. L. (1990). Interaction between light quality and light quantity in the photoregulation of anthocyanin production. Plant Physiology, 92(4), 1191-1195. google scholar
• Mittler, R. (2006). Abiotic stress, the field environment and stress combination. Trends in Plant Science, 11(1), 15-19. google scholar
• Nakano, Y., & Asada, K. (1987). Purification of ascorbate peroxi-dase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiology, 28(1), 131-140. google scholar
• Noctor, G., & Foyer, C. H. (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Biology, 49(1), 249-279. google scholar
• Noctor, G., Reichheld, J. P., & Foyer, C. H. (2018). ROS-related redox regulation and signaling in plants. Semin Cell Dev Biol, 80, 3-12. doi:10.1016/j.semcdb.2017.07.013 google scholar
• Oufir, M., Schulz, N., Vallikhan, P. S. S., Wilhelm, E., Burg, K., Hausman, J.-F., . . . Guignard, C. (2009). Simultaneous measure-ment of proline and related compounds in oak leaves by high-performance ligand-exchange chromatography and electrospray ionization mass spectrometry for environmental stress studies. Journal of Chromatography A, 1216(7), 1094-1099. google scholar
• Ozden, H., & Bayçu, G. (2004). Cadmium exposure and changes in some physiological parameters of Quercus robur ssp. robur L. (common oak) and Acer negundo L. (box elder) seedlings. Fresenius Environmental Bulletin, 13, 268-273. google scholar
• Öpik, H., & Rolfe, S. A., (2005) The physiology of flowering plants. Cambridge, UK: Cambridge University Press. google scholar
• Pellegrini, E., Hoshika, Y., Dusart, N., Cotrozzi, L., Gerard, J., Nali, C., Paoletti, E. (2019). Antioxidative responses of three oak species under ozone and water stress conditions. Science of the Total Environment, 647, 390-399. google scholar
• Perales-Vela, H. V., Gonzalez-Moreno, S., Montes-Horcasitas, C., & Canizares-Villanueva, R. O. (2007). Growth, photosynthetic and respiratory responses to sub-lethal copper concentrations in Scenedesmus incrassatulus (Chlorophyceae). Chemosphere, 67(11), 2274-2281. google scholar
• Rao, K. M., Raghavendra, A. S., & Reddy, K. J. (2006). Physiology and molecular biology of stress tolerance in plants: Springer Science & Business Media. google scholar
• Santamarina, S., Montesinos, D., Alfaro-Saiz, E., & Acedo, C. (2022). Drought affects the performance of native oak seedlings more strongly than competition with invasive crested wattle seedlings. Plant Biology, 24(7), 1297-1305. google scholar
• Schwanz, P., Picon, C., Vivin, P., Dreyer, E., Guehl, J.-M., & Polle, A. (1996). Responses of antioxidative systems to drought stress in pendunculate oak and maritime pine as modulated by elevated CO2. Plant Physiology, 110(2), 393-402. google scholar
• Simova-Stoilova, L. P., Lopez-Hidalgo, C., Sanchez-Lucas, R., Valero-Galvan, J., Romero-Rodriguez, C., & Jorrin-Novo, J. V. (2018). Holm oak proteomic response to water limitation at seedling es-tablishment stage reveals specific changes in different plant parts as well as interaction between roots and cotyledons. Plant Science, 276, 1-13. google scholar
• Suzuki, N., & Mittler, R. (2006). Reactive oxygen species and tempera-ture stresses: a delicate balance between signaling and destruction. Physiologia Plantarum, 126(1), 45-51. google scholar
• Thomas, F. M., Blank, R., & Hartmann, G. (2002). Abiotic and biotic factors and their interactions as causes of oak decline in Central Europe. Forest Pathology, 32(4-5), 277-307. google scholar
• Velikova, M., Bankova, V., Sorkun, K., Houcine, S., Tsvetkova, I., & Kujumgiev, A. (2000). Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Zeitschrift für Naturforschung C, 55(9-10), 790-793. google scholar
• Xiong, S., Wang, Y., Chen, Y., Gao, M., Zhao, Y., & Wu, L. (2022). Effects of drought stress and rehydration on physiological and biochemical properties of four oak species in China. Plants, 11(5), 679. google scholar
• Yordanov, I., Velikova, V., & Tsonev, T. (2000). Plant responses to drought, acclimation, and stress tolerance. Photosynthetica, 38, 171-186. google scholar