Antibacterial, Antifungal, and Cytotoxic Effects of Seseli tortuosum L. Root Extracts

Abstract

Background and Aims: This study investigated the antibacterial, antifungal, and cytotoxic effects of Seseli tortuosum L. root extracts, a member of the Apiaceae family known for its diverse pharmacological activities. The genus Seseli L. is historically used in traditional medicine, and the Seseli species generally exhibit anti-inflammatory, antifungal, antibacterial, and cytotoxic properties. Methods: Extraction involved maceration and Soxhlet methods using dichloromethane (DCM), ethyl acetate (EtOAc), and methanol (MeOH). The antibacterial activity was assessed via broth microdilution assays, and the antifungal activity was evaluated using the Poison PDA technique against Aspergillus parasiticus, Fusarium moniliforme, and Fusarium culmorum. The cytotoxic effects on human cancer cell lines (MCF7, PC3, A549) and a normal embryonic kidney cell line (HEK293) were determined using the MTT assay. Results: Cytotoxic assays indicated that the DCM macerate extract had the most potent effects on the PC3 cell line, with an IC₅₀ value of 82.78 ± 0.99 µg/mL, showing higher selectivity compared to the MCF7 and A549 cell lines. The antibacterial tests revealed that the DCM and EtOAc extracts significantly inhibited E. Faecalis, MIC values calculated as 0.3125 mg/mL for both extracts. The antifungal activity results indicated that the Soxhlet DCM extract exhibited the most consistent antifungal activity, particularly against F. culmorum and F. moniliforme %44 inhibition for both, whereas the MeOH Soxhlet extract showed strain specific effects. The extracts displayed substantial antibacterial, antifungal, and cytotoxic activities. Conclusion: Findings of this study underscore the need for further biological and phytochemical investigations to fully explore the potential of the S. tortuosum extracts as cytotoxic therapeutics and antimicrobial agents.

Keywords

• Seseli
• Apiaceae
• Antibacterial
• Antifungal
• Cytotoxicity

References

1. Badalamenti, N., Vaglica, A., Ilardi, V., & Bruno, M. (2022). The chemical composition of essential oil from Seseli tortuosum subsp. tortuosum and S. tortuosum subsp. maritimum (Apiaceae) aerial parts growing in Sicily (Italy). Natural Product Research, 37(20), 3519–3524. https://doi.org/10.1080/14786419.2022.2078819 google scholar
2. Bader, A., Caponi, C., Cioni, P. L., Flamini, G., & Morelli, I. (2003). Acorenone in the essential oil of flowering aerial parts of Seseli tortuosum L. Flavour and Fragrance Journal, 18(1), 57–58. https://doi.org/10.1002/FFJ.1154 google scholar
3. Çaşkurlu, A., Karavuş, Ş. N., Biltekin, S. N., Helvacı, E. Z., & Karadağ, A. E. (2023). In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. Journal of Research in Pharmacy, 27(5)(27(5)), 2115–2123. https://doi. org/10.29228/jrp.490 google scholar
4. Cinar, A. S., Bakar-Ates, F., & Onder, A. (2020). Seseli petraeum M. Bieb. (Apiaceae) significantly inhibited cellular growth of A549 lung cancer cells through G0/G1 Cell Cycle Arrest. Anais Da Academia Brasileira de Ciências, 92(4), 1–11. https:// doi.org/10.1590/0001-3765202020191533 google scholar
5. Clinical and Laboratory Standards Institute (CLSI). (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard. 7th Ed. https://clsi.org/media/ 1928/m07ed11_sample.pdf google scholar
6. Dogan, E., Duman, H., Tosun, A., Kürkçüoglu, M., & Baser, K. H. C. (2011). Essential oil composition of the fruits of Seseli resinosum Freyn et Sint. and Seseli tortuosum L. growing in Turkey. Journal of Essential Oil Research, 18(1), 57–59. https://doi.org/10.1080/10412905.2006.9699385 google scholar
7. Gonçalves, M. J., Tavares, A. C., Cavaleiro, C., Cruz, M. T., Lopes, M. C., Canhoto, J., & Salgueiro, L. (2012). Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops and Products, 39(1), 204–209. https://doi.org/10.1016/J.INDCROP.2012.02.025 google scholar
8. Güner, A. , & A. S. (Eds. ). (2012). Türkiye bitkileri listesi:(damarlı bitkiler). Nezahat Gökyiǧit Botanik Bahçesi Yayınları. google scholar

9. GÜNER, E. D., & Duman, H. (2013). The revision of genus Seseli (Umbelliferae) in Turkey. Turkish Journal of Botany, 37(6), 1018–1037. google scholar
10. Hu, C. Q., Lee, K. H., & Chang, J. J. (1990). Antitumor agents, 115.1 seselidiol, a new cytotoxic polyacetylene from seseli mairei. Journal of Natural Products, 53(4), 932– 935. https://doi.org/10.1021/NP50070A022/ASSET/NP50070A022.FP.PNG_V03 google scholar
11. Kaya, A., Demirci, B., Hüsnü, K., & Ba6er, C. (2003). The essential oil of Seseli tortuosum L. growing in Turkey. Wiley Online Library, 18(2), 159–161. https://doi.org/10. 1002/ffj.1162 google scholar
12. Küpeli, E., Tosun, A., & Yesilada, E. (2006). Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. Journal of Ethnopharmacology, 104(3), 310–314. https://doi.org/10.1016/J.JEP.2005.09.021 google scholar
13. Leontia, M., Casu, L., Solinas, M. N., Cottiglia, F., Caboni, P., Floris, C., Gertsch, J., & Saba, A. R. (2010). A chromone from Seseli praecox (Apiaceae). Natural Product Communications, 5(4), 551–554. https://doi.org/10.1177/1934578X1000500411 google scholar
14. Maleki, M., Yousefi, K., Asadi, M. H., & Mirtadzadini, S. M. (2024). Seseli transcaucasicum (Schischk.) Pimenov & sdobnina leaf and seed ethanolic and methanolic extracts: cytotoxic activity on MCF7 cell line and antioxidant properties. Journal of Medicinal Plants and By-Products, 13(2), 459–467. https://doi.org/10.22034/ JMPB.2023.362345.1562 google scholar
15. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1–2), 55–63. https://doi.org/10.1016/0022-1759(83)90303-4 google scholar
16. Ogbebor, O. N., Adekunle, A. T., & Evueh, G. A. (2008). Inhibition of Drechslera heveae (Petch) MB Ellis, causal organism of Bird’s eye spot disease of rubber (Hevea brasiliensis Muell Arg.) using plant extracts. African Journal of General Agriculture, 4(1), 19-26. http://ojs.klobexjournals.com/index.php/ajga/article/ view/693 google scholar
17. Önder, A., Çinar, A. S., Bakar-Ateş, F., Noguera-Artiaga, L., & Carbonell-Barrachina, Á. A. (2021). Chemical composition and cytotoxic potency of essential oil from Seseli petraeum M. Bieb. (Apiaceae). Journal of Research in Pharmacy, 25(3), 249–257. https://doi.org/10.29228/JRP.15 google scholar
18. Önder, A., Çinar, A. S., Yilmaz Sarialtin, S., İzgi, M. N., & Çoban, T. (2020). Evaluation of the Antioxidant Potency of Seseli L. Species (Apiaceae). Turkish Journal of Pharmaceutical Sciences, 17(2), 197. https://doi.org/10.4274/TJPS.GALENOS. 2019.80488 google scholar
19. Petrović, G. M., Stamenković, J. G., Jovanović, O. P., & Stojanović, G. S. (2019). Chemical Compositions of the Essential Oils and Headspace Volatiles of Seseli peucedanoides Plant Parts, 14(5). https://doi.org/10.1177/1934578X19850691 google scholar
20. POWO. (2024, September 2). Plants of the World Online Kew Science. Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet, Apiaceae. https://powo.science.kew.org/taxon/urn:lsid:ipni.org: names:30000180-2#synonyms google scholar
21. Schmitz H. (1930). Poisoned food technique. Industrial and Engineering Chemistry- Analytical Edition, 2(4), 361–353. https://scholar.google.com/scholar?hl=tr& as_sdt=0%2C5&q=chmitz%2C+H.+Poisoned+food+technique.+Industrial+and+ Engineering+Chemistry-Analytical+Edition.+1930%2C+2%2C+361-363.&btnG= google scholar
22. Tandan, S. K. , Chandra, S., Tripathi, H. C., & Jawahar, Lal. J. (1990). Pharmacological actions of seselin, a coumarin from Seseli indicum seeds. Fitoterapia, 61(4), 360–363. https://www.cabdirect.org/cabdirect/abstract/19910302135 google scholar