In vitro inhibitory potential of Amaranthus lividus L. against therapeutic target enzyme

Year 2022, , 73 - 79, 28.04.2022

Gozde Hasbal Çelikok , Tuğba Yılmaz Özden , Özlem Saçan Ayse Can , Refiye Yanardağ

https://doi.org/10.26650/IstanbulJPharm.2022.964803

Abstract

Background and Aims: The search for new enzyme inhibitors in plants is attractive because they can be used as drugs in the treatment of various diseases. Amaranthus spp. (Amaranthaceae) includes about 70 different species, some of which are ed- ible and some of which are used in traditional medicine to treat various ailments. Amaranthus lividus L. is a vegetable whose stems and leaves are used for human consumption in Turkey.Methods: In this study, the in vitro enzyme inhibition potential of A. lividus on α-amylase, α-glucosidase, acetylcholinester- ase (AChE), elastase, lipase, neuraminidase and tyrosinase was investigated for the first time. For this purpose, water extract of A. lividus was prepared. The tests of enzyme inhibitory activity were carried out by spectrophotometric and fluorometric methods.
Results: The water extract showed significant α-glucosidase and α-amylase inhibitory activities. Also, it displayed good elas- tase, lipase and tyrosinase inhibitory activities. However, it exhibited low inhibitory activity on AChE and neuraminidase.Conclusion: The plant and its active constituents may be used as an antidiabetic enzyme inhibitor with future phytochemical constituent analysis.

Keywords

α-Amylase, α-Glucosidase, Acetylcholinesterase, Elastase, Lipase, Tyrosinase

Supporting Institution

This work was supported by the Scientific Research Projects Coordination Unit of Istanbul University [Project numbers: UDP-47164 and BEK-2017-24682].

Thanks

The authors are thankful to Professor Asuman Baytop from the identification of Amaranthus lividus L.

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