Background and Aims: A new sensitive and selective method for the determination of sultamicillin in bulk and pharmaceutical preparations has been developed. Methods: The developed method is based on the derivatization of sultamicillin with 4-chloro-7-nitrobenzofurazan in pH 9.0 borate buffer at 70ºC for 60 min. The derivative was measured spectrophotometrically at 432 nm after extraction with chloroform. Results: The method was validated by linearity, LOD, LOQ, accuracy, precision and recovery parameters. The linear range for sultamicillin was 10-50 µg.mL-1 with a correlation coefficient of 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.47 and 4.41 µg/mL-1, respectively. Conclusion: The suggested method was validated, and can be applied for routine sultamicillin analysis in dosage formsfor industrial analysis.
4-chloro-7-nitrobenzofurazan sultamicillin spectrophotometry validation
Birincil Dil | İngilizce |
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Konular | Eczacılık ve İlaç Bilimleri, Sağlık Kurumları Yönetimi |
Bölüm | Original Article |
Yazarlar | |
Yayımlanma Tarihi | 1 Nisan 2020 |
Gönderilme Tarihi | 11 Temmuz 2019 |
Yayımlandığı Sayı | Yıl 2020 Cilt: 50 Sayı: 1 |