Short-term adaptive metabolic response of Escherichia coli to ciprofloxacin exposure

Yıl 2021, Cilt: 51 Sayı: 1, 79 - 84, 30.04.2021

Engin Koçak Ceren Özkul

Öz

Background and Aims: Antibiotic resistance is one of the most critical global health problems. Understanding the pathogen-antibiotic relationship at molecular level could lead to the discovery of new routes to overcome antibiotic resistance. In our present work, we evaluated early responses of E. coli against ciprofloxacin within 30 min by analyzing metabolome structure. Our main goal was to understand the initial steps of the adaptation and resistance process of pathogens under antibiotic stress. Methods: Metabolomics analysis was performed by processing GC/MS and followed with the MS-DIAL metabolomics platform. In addition, Metaboanalyst 4.0 and the KEGG database were used for statistical and pathway analysis. Results: In total, 207 metabolites were identified while 47 metabolites were significantly different under ciprofloxacin stress condition. Pathway analysis showed that amino acid, fatty acid, and aminoacyl-tRNA metabolisms were altered as an effect of ciprofloxacin at 30 min. Conclusion: Our results suggest that the understanding of bacterial metabolism in early phase bacterial responses to antibiotics could be key to reducing the adaptation and resistance process.

Anahtar Kelimeler

Metabolomics, GC/MS, ciprofloxacin, E. coli

Kaynakça

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