Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach

Yıl 2021, Cilt: 51 Sayı: 3, 372 - 377, 29.12.2021

Zeynep Doğan , Yasin Genç , Şebnem Harput , Asuman Karadeniz Pekgöz , İclal Saraçoğlu

Öz

Background and Aims: Genus Veronica (Plantaginaceae) is represented by 79 species in Turkish flora, 26 of which are endemic. Veronica species have a variety of uses including diuretic, anticancer, and rheumatic pains, wounds, and respiratory problems. According to phytochemical studies, Veronica species contain predominantly iridoid glucosides with some phenylethanoid and flavonoid glycosides. Methods: The aqueous extract of Veronica peduncularis M.Bieb. was tested for its cytotoxic activity on human rhabdomyosarcoma (RD) and human epidermoid carcinoma (HEp-2) cell lines using the MTT method. Chemical profile of the extract was determined by HPLC-DAD, and isolation studies were conducted. Results: The extract was found to show concentration-dependent cytotoxicity against tested cell lines. In addition, a comparison of the iridoid fraction of Veronica peduncularis with previously isolated iridoid glucosides on the HPLC-DAD system, showed the presence of aucubin, amphicoside, veratroyl catalpol, and veronicoside in this fraction. On the other hand, isolation and structure elucidation of plantamajoside and 4'-O-methylisoscutellarein-7-O-2''-O-(6'''-O-acetyl-ß-D-allopyranosyl)- ß-D-glucopyranoside from the phenolic fractions were performed by serial chromatographic and spectroscopic methods. Conclusion: To the best of our knowledge, this is the first cytotoxic activity and phytochemical study on the titled plant. The presence of iridoid glucosides and 8-hydroxyflavone glycosides is important for the chemotaxonomy of the genus Veronica.

Anahtar Kelimeler

4, HPLC, iridoid glucosides, phenolics, plantamajoside, Veronica

Destekleyen Kurum

The Scientific and Technological Research Council of Turkey (TUBITAK)

Proje Numarası

108T518

Kaynakça

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