Relationship of thiol/disulphide homeostasis with oxidative stress parameters in non-diabetic, prediabetic and type 2 diabetic Turkish women

Yıl 2022, Cilt: 52 Sayı: 2, 121 - 128, 30.08.2022

Ayse Ceylan Hamamcıoglu , Çınar Severcan , Taner Bayraktaroğlu

https://doi.org/10.26650/IstanbulJPharm.2022.1011516

Öz

Background and Aims: Deterioration of thiol/disulphide homeostasis (TDH) is found in several diseases, including diabetes. This study aimed to examine the association between TDH and oxidative stress parameters in nondiabetics, prediabetics and newly diagnosed type 2 diabetics.
Methods: A total of 26 non-diabetic, 24 prediabetic and 19 type 2 diabetic women were involved in our study. They all applied to Zonguldak Bulent Ecevit University, Health Practice and Research Center, Endocrinology and Metabolism Diseases, Diabe- tes Polyclinic to be tested for type 2 diabetes mellitus. The demographic and laboratory data were collected from the patient files. Oxidative stress parameters and dynamic TDH were investigated using ELISA kits.
Results: Total oxidant status (TOS), total thiol and disulphide levels were significantly higher in type 2 diabetics than the non-diabetics (24.24±14.93 versus 14.14±12.19, 646.47±75.51 versus 470.88±180.85, and 179.32±51.24 versus 91.85±40.29, respectively). In type 2 diabetics, a positive correlation between TOS and native thiol, total thiol and disulphide levels was found (P=0.000). In prediabetics, a significant positive correlation was found between total antioxidant capacity and total thiol levels (P<0.05), and also between arylesterase and native and total thiol levels (P<0.05).

Conclusion: The elevation of oxidative stress and the deterioration of TDH might cause the formation of symptoms related to high blood glucose levels in type 2 diabetics.

Anahtar Kelimeler

Sulfhydryl compounds, Diabetes mellitus, Prediabetic state, Oxidative stress

Destekleyen Kurum

This work was supported by the Zonguldak Bulent Ecevit University Scientific Research Project Unit

Proje Numarası

BAP 2018-43085703-02

Teşekkür

We would like to thank Ebru Boz Uzaldı, diabetes nurse, for collecting blood samples from each individual and keeping the informed consent forms.

Kaynakça

• Agan, V., Celik, H., Eren, M. A., Agan, F. Z., Erel, O., Neselioglu, S. … Gonel, A. (2019). An investigation of oxidative stress and thiol/ disulphide homeostasis in graves’ disease. Medicina (Kaunas), 55, 275-287. http://dx.doi.org/10.3390/medicina55060275.
• Aktun, L. H., Aykanat, Y., Erel, O., Neselioglu, S., & Olmuscelik, O. (2018). A study over thiol disulphide homeostasis in cord blood in women with gestational diabetes. Jounal of Family and Reproduc- tive Health, 12, 217-222.
• Aldemir, M., Karaguzel, E., Okulu, E., Gudeloglu, A., Ener, K., Ozayar, A., & Erel, O. (2015). Evaluation of oxidative stress status and anti- oxidant capacity in patients with renal cell carcinoma. Central Eu- ropean Journal of Urology, 68, 415-420. http://dx.doi.org/10.5173/ ceju.2015.656.
• Ates, I., Kaplan, M., Inan, B., Alisik, M., Erel, O., Yilmaz, N., & Guler, S. (2015). How does thiol/disulphide homeostasis change in pre- diabetic patients? Diabetes Research and Clinical Practice, 110(2), 166–171. https://doi.org/10.1016/j.diabres.2015.09.011.
• Ates, I., Kaplan, M., Yuksel, M., Mese, D., Alisik, M., Erel, O. … Guler, S. (2016). Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxida- tion. Endocrine, 51, 47-51. http://dx.doi.org/10.1007/ s12020-015-0784-6.
• Aydogan, S., Akduman, H., Dilli, D., Koyuncu, E., Citli, R., Erel, O … Zenciroglu A. (2021). The role of thiol-disulphide homeostasis in neonatal sepsis. The Journal of Maternal-Fetal & Neonatal Medicine, 34, 1522-1528. http://dx.doi.org/10.1080/14767058.2019.1638904.
• Bandeira, S., Guedes, G., da Fonseca, L. J., Pires, A. S., Gelain, D. P., Moreira, J. C. … Goulart, M. O. (2012). Characterization of blood oxidative stress in type 2 diabetes mellitus patients: increase in lipid peroxidation and SOD activity. Oxidative Medicine and Cellu- lar Longevity 2012, 819310. https://doi.org/10.1155/2012/819310.
• Dziegielewska-Gesiak, S., Wysocka, E., Michalak, S., Nowakowska- Zajdel, E., Kokot, T., & Muc-Wierzgon M. (2014). Role of lipid per- oxidation products, plasma total antioxidant status, and Cu-, Zn- superoxide dismutase activity as biomarkers of oxidative stress in elderly prediabetics. Oxidative Medicine and Cellular Longevity, 2014, 987303. https://doi.org/10.1155/2014/987303.
• Eckerson, H.W., Wyte, C.M., & La Du, B.N. (1983). The human serum paraoxonase/arylesterase polymorphism. The American Journal of Human Genetics, 35, 1126–1138.
• Eljaoudi, R., Elomri, N., Laamarti, M., Cherrah, Y., Amezyane, T., Ghafir D, & Ibrahimi, A. (2017). Antioxidants status in type 2 dia- betic patients in Morocco. Turkish Journal of Medical Sciences, 47, 782-788. http://dx.doi.org/10.3906/sag-1512-110.
• Emre, S., Akoglu, G., Metin, A., Demirseren, D.D., Isikoglu, S., Oz- tekin, A. & Erel, O. (2016). The oxidant and antioxidant status in pityriasis rosea. Indian Journal of Dermatology, 61, 118-138. http:// dx.doi.org/10.4103/0019-5154.174073.
• Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more sta- ble ABTS radical cation. Clinical Biochemistry, 37, 277-285. http:// dx.doi.org/10.1016/j.clinbiochem.2003.11.015.
• Erel, O. (2005). A new automated colorimetric method for mea- suring total oxidant status. Clinical Biochemistry, 38, 1103-1111. http://dx.doi.org/10.1016/j.clinbiochem.2005.08.008.
• Erel, O., & Neselioglu, S. (2014). A novel and automated assay for thiol/disulphide homeostasis. Clinical Biochemistry, 47, 326–332. http://dx.doi.org/10.1016/j.clinbio chem.2014.09.026.
• Erel, O., & Erdogan, S. (2020). Thiol-disulphide homeostasis: an integrated approach with biochemical and clinical aspects. Turk- ish Journal of Medical Sciences, 50(SI-2), 1728-1738. http://dx.doi. org/10.3906/sag-2003-64.
• Ergin, M., Aydin, C., Yurt, E.F., Cakir, B., Erel, O. (2020). The variation of disulphides in the progression of type 2 diabetes mellitus. Ex- perimental and Clinical Endocrinology & Diabetes, 128 (2), 77-81. http://dx.doi.org/10.1055/s-0044-100376.
• Garber, A.J., Abrahamson, M.J., Barzilay, J.I., Blonde, L., Bloomgar- den, Z.T., Bush, M.A. … Umpierrez,G.E. (2019). Consensus State- ment by The American Association of Clinical Endocrinologists and American College of Endocrinology on the Comprehen- sive Type 2 Diabetes Management Algorithm - 2019 Execu- tive Summary. Endocrinology Practice, 25, 69-100. http://dx.doi. org/10.4158/CS-2018-0535.
• Gulpamuk, B., Tekin, K., Sonmez, K., Inanc, M., Neselioglu, S….Yilmazbas, P. (2018). The significance of thiol/disulphide homeo- stasis and ischemia- modified albumin levels to assess the oxida- tive stress in patients with different stages of diabetes mellitus. Scandinavian Journal of Clinical Laboratory Investigation, 78, 136-142. http://dx.doi.org/10.1080/003655132017.
• 1422540.
• Hamamcioglu, A.C. (2017). The role of oxidative stress and antioxi- dants in diabetes mellitus. Turkish Journal of Diabetes and Obesity, 1, 7-13. http://dx.doi.org/10.25048/tjdo.2017.2.
• Harma, M., Harma, M., & Erel, O. (2003). Increased oxidative stress in patients with hydatidiform mole. Swiss Medical Weekly, 133, 563-566.
• Karatas, M., Ozis, T. N., Buyuksekerci, M., Gunduzoz, M., Ozakıncı, O. G., … Erel, O. (2019). Thiol-disulphide homeostasis and ischemia- modified albumin levels as indicators of oxidative stress in weld- ers’ lung disease. Human & Experimental Toxicology, 38, 1227-1234. http://dx.doi.org/10.1177/0960327119871093.
• Khetan, A. K., & Rajagopalan, S. (2018). Prediabetes. Canadian Journal of Cardiology, 34, 615-623. http://dx.doi.org/10.1016/j. cjca.2017.12.030
• Kilinc, F., Sener, S., Akbas, A., Metin, A., Kirbas, S., Neselioglu S., & Erel, O. (2016). Oxidative stress parameters in localized sclero- derma patients. Archives of Dermatological Research, 308, 625-629. http://dx.doi.org/10.1007/s00403-016-1682-3.
• Kosecik, M., Erel, O., Sevinc, E., & Selek, S. (2005). Increased oxida- tive stress in children exposed to passive smoking. International Journal of Cardiology, 100, 61–64. http://dx.doi.org/10.1016/j. ijcard.2004.05.069.
• Lovic, D., Piperidou, A., Zografou, I., Grassos, H., Pittaras, A., & Manolis, A. (2020). The growing epidemic of diabetes mellitus. Current Vascular Pharmacology, 18, 104-109. http://dx.doi.org/10.2174/1570161117666190405165911.
• Nair, A., & Nair, B. J. (2017). Comparative analysis of the oxidative stress and antioxidant status in type II diabetics and nondiabetics: A biochemical study. Journal of Oral and Maxillofacial Pathology, 21, 394-401. http://dx.doi.org/10.4103/jomfp. JOMFP_56_16.
• Noack, B., Aslanhan, Z., Boué, J., Petig, C., Teige, M., Schaper, F. … Hannig, C. (2013). Potential association of paraoxonase-1, type 2 diabetes mellitus, and periodontitis. Journal of Periodontology, 84(5), 614–623. https://doi.org/10.1902/jop.2012.120062.
• Rosenblat, M., Sapir, O., & Aviram, M. (2008). The paraoxonases: their role in disease development and xenobiotic metabolism. In B. Mack- ness, M. Mackness, M. Aviram & G. Paragh (Eds.), Glucose inactivates Paraoxonase 1 (PON1) and displaces it from high density lipoprotein (HDL) to a free PON1 form (pp.35-51). New York, USA: Springer.
• Sies, H. (1997). Oxidative stress: oxidants and antioxidants. Experi- mental Physiology, 82, 291-295. http://dx.doi.org/10.1113/exp- physiol.1997.sp004024.
• Sonmez, M. G., Kozanhan, B., Deniz, C. D., Iyisoy, M. S., Kilinc, M. T., Ecer, G. … Erel, O. (2019). Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in patients with urolithia- sis. Investigative and Clinical Urology, 60, 258-266. http://dx.doi. org/10.4111/icu.2019.60.4.258.
• Sozer, V., Himmetoglu, S., Korkmaz, G. G., Kaya, S., Aydin, S., Yumuk, V. … Uzun, H. (2014). Paraoxonase, oxidized low density lipopro- tein, monocyte chemoattractant protein-1 and adhesion mol- ecules are associated with macrovascular complications in pa- tients with type 2 diabetes mellitus. Minerva Medica, 105, 237-244.
• Tas, S., Sarandol, E., & Dirican, M. (2014). Vitamin B6 supple- mentation improves oxidative stress and enhances serum paraoxonase/arylesterase activities in streptozotocin-induced diabetic rats. Scientific World Journal,2014, 351598. https://doi. org/10.1155/2014/351598.
• Topal, I., Mertoglu, C., Surucu Kara, I., Gok, G., & Erel, O. (2019). Thi- ol-disulphide homeostasis, serum ferroxidase activity, and serum ischemia modified albumin levels in childhood iron deficiency anemia. Fetal and Pediatric Pathology, 38, 484-489. http://dx.doi.or g/10.1080/15513815.2019.1627626.
• Unal, E., Eris, C., Kaya, B., Uzun, H., Cavdar, F., …,Titiz, I. (2012). Paraoxonase and arylesterase activities, lipid profile, and oxida- tive damage in experimental ischemic colitis model. Gastro- enterology Research and Practice, 2012, 2012-2017. https://doi. org/10.1155/2012/979506.
• Wu, G., Fang, Y-Z., Yang, S., Lupton, J.R., & Turner, N.D. (2004). Gluta- thione Metabolism and Its Implications for Health. The Journal of Nutrition, 134(3), 489–492. https://doi.org/10.1093 /jn/134.3.489.