Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells

Yıl 2024, Cilt: 54 Sayı: 1, 89 - 95, 30.04.2024

Zeynep Özgen Bölükbaşı , Cemre Özkanca , Ayşe Seher Birteksöz Tan

https://doi.org/10.26650/IstanbulJPharm.2024.1431886

Öz

Background and Aims: Streptococcus anginosus and Candida albicans exert negative effects on oral health and can cause biofilm formation on tooth surfaces. Partial or total denture surfaces used by people who have lost their teeth due to various reasons are sensitive to microorganism invasion and biofilm formation, similar to tooth surfaces. Therefore, this study was conducted to investigate the antibiofilm activities of various denture cleaning tablets (DCTs) used to disinfect dentures against S. anginosus clinical isolates and C. albicans standard strain and their cytotoxic effects against oral epithelial cells.

Methods: The biofilm-forming abilities of strains were determined using the crystal violet assay. The modified time-killing curve (TKC) method was used to evaluate the dynamic bactericidal or fungicidal activities of DCTs against biofilms of S. anginosus isolates and C. albicans ATCC 10231. In vitro cytotoxicity experiments of DCTs on human gingival fibroblast cell lines (HGF-1) were also conducted using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide staining method.

Results: Overall, 18.18% and 36.36% of S. anginosus isolates were strong and moderate biofilm formers, respectively. C. albicans ATCC 10231 also formed a strong biofilm. The TKC analysis revealed that all the examined DCTs inhibited almost all living cells in mature biofilms at every time point. The cytotoxic activities of DCTs against HGF-1 cell lines were in the range of 93%–95% at their direct usage concentrations.

Conclusion: DCTs exhibit rapid and strong activity against biofilms, which is extremely important for biofilm-related infections. Nevertheless, it is necessary to consider the cytotoxic effects of DCTs on HGF-1 cells for consumers’ oral healt.

Anahtar Kelimeler

Biofilm, denture cleaning tablet, cytotoxic effects, Streptococcus anginosus

Kaynakça

• Andrighetti, Fröhner., C.R., Antonio, R.V., Creczynski-Pasa, T.B., Barardi, C. R.M., Simöes, C.M.O. (2003). Cytotoxicity and po-tential antiviral evaluation of violacein produced by Chromobac-terium violaceum. Memorias do Instituto Oswaldo Cruz, 98, 843848. google scholar
• Apratim, A., Shah, S. S., Sinha, M., Agraval M, Chhaparia, N., Abubakkar, A. (2013). Denture hygiene habits among elderly pa-tients wearing complete dentures. Journal of Contemporary Den-tal Practice, 14, 1161-1164. google scholar
• Budtz-Jorgensen E., Loe, H. (1972). Chlorhexidine as a denture disin-fectant in the treatment of denture stomatitis. Scandinavian Jour-nal of Dental Research, 80, 457-464. google scholar
• Dosler, S., Karaaslan, E. (2014). Inhibition and destruction of Pseu-domonas aeruginosa biofilms by antibiotics and antimicrobial pep-tides. Peptides, 62, 32-37. google scholar
• Emami, E., Kabawat, M., Rompre, P.H., Feine, J.S. (2014). Linking evidence to treatment for denture stomatitis: A meta-analysis of randomized controlled trials. Journal of Dentistry, 42, 99-106. google scholar
• Gendreau, L., & Zg, L. (2011). Epidemiology and etiology of denture stomatitis. Journal of Prosthodontics, 20, 251-260. google scholar
• Heller, D., Helmerhorst, E. J., Gower, A. C., Siqueira, W. L., Paster, B. J., & Oppenheim, F. G. (2016). Microbial Diversity in the Early In Vivo-Formed Dental Biofilm. Applied and Environmental Microbiology, 82, 1881-1888. google scholar
• Jenkinson, H.F. (1994). Cell surface protein receptors in oral strepto-cocci. FEMS Microbiology Letters,121(2),133-140. google scholar
• Jose, A., Coco, B. J., Milligan, S., Young, B., Lappin, D.F., Bagg, J....Ramage, G. (2010). Reducing the incidence of denture stomatitis: Are denture cleansers sufficient? Journal of Prosthodontics, 19, 252-257. 10.1111/j.1532-849X.2009.00561.x google scholar
• Li, J., Helmerhorst, E. J., Leone, C. W., Troxler, R. F., Yaskell, T., Haffajee, A. D., Socransky, S. S., & Oppenheim, F. G. (2004). Identification of early microbial colonizers in human dental biofilm. Journal of Applied Microbiology, 97, 1311-1318. https://doi.org/10.1111/j.1365-2672.2004.02420.x google scholar
• Martori, E., Ayuso-Montero, R., Martinez-Gomis, J., Vinas, Miquel., Peraire, M. (2014). Risk factors for denture-related oral mucosal lesions in a geriatric population. The Journal of Prosthetic Den-tistry, 111, 273-279. doi: 10.1016/j.prosdent.2013.07.015. google scholar
• Nirwati, H., Sinanjung, K., Fahrunissa, F., Fernando, W., Napitupulu, S., Hati, V.P.... Nuryastuti, T. (2019). Biofilm formation and an-tibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia. BMC, 13, 1-8 .https://doi.org/10.1186/s12919-019-0176-7 google scholar
• Nobbs, A. H., Lamont, R. J., & Jenkinson, H. F. (2009). Streptococcus adherence and colonization. Microbiology and Molecular Biology Reviews, 73, 407-450. https://doi.org/10.1128/MMBR.00014-09. google scholar
• Orgini, D. B., Silva-Lovato, C. H., de Souza, R. F., & et al. (2012). Abrasiveness of conventional and specific denture-cleansing den-tifrices. Brazilian Dental Journal, 23, 154-159. google scholar
• Ramage, G., Saville, S.P., Thomas, D. P., et al. (2005). Candida biofilms: An update. Eukaryotic Cell, 4, 633-688. google scholar
• Ramage, G., O’Donnell, L., Sherry, L., Culshaw, S., Bagg, J., Czesnikiewicz-Guzik, M., Brown, C.... Robertson, D. (2018). Im-pact of frequency of denture cleaning on microbial and clinical pa-rameters - a bench to chairside approach. Journal of Oral Microbi-ology, 11, 1-15. https://doi.org/10.1080/20002297.2018.1538437 google scholar
• Ritz, H. L. (1967). Microbial population shifts in developing human dental plaque. Archives of Oral Biology, 12, 1568-1570. google scholar
• Ruhl, S., Eidt, A., Melzl, H., Reischl, U., & Cisar, J. O. (2014). Probing of microbial biofilm communities for coadhesion part-ners. Applied and Environmental Microbiology, 80, 6583-6590. https://doi.org/10.1128/AEM.01826-14. google scholar
• Shankar, T., Gowd, S., Suresan, V., Mantri, S., Saxena, S., Mishra, P,. Panday, P. (2017) Denture Hygiene Knowledge and Practices among Complete Denture Wearers attending a Postgraduate Den-tal Institute. Journal of Contemporary Dental Practice, 18, 714721. google scholar
• Srinivasan, M., & Gulabani, M. (2010). A microbiological evaluation of the use of denture cleansers in combination with an oral rinse in complete denture patients. Indian Journal of Dental Research, 21, 353-356. google scholar
• Takamiya, A.S.; Monteiro, D.R.; Barâo, V.A.R.; Barbosa, D.B.; Pero, A.C, Compagnoni, M.A. (2011). Complete denture hy-giene and nocturnal wearing habits among patients attending the Prosthodontic Department in a Dental University in Brazil. Gerodontology, 28, 91-96. google scholar
• Verran, J., Jackson, S., Coulthwaite, L., & et al. (2014). The effect of dentifrice abrasion on denture topography and the subsequent retention of microorganisms on abraded surfaces. Journal of Pros-thetic Dentistry, 112, 1513-1522. google scholar