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Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli

Yıl 2008, Cilt: 2 Sayı: 2, 19 - 22, 01.07.2008

Murat Kasap Sinem Torol Gulcin Gacar

Öz

During construction of genomic libraries from various bacteria, we experienced considerably different transformation effi ciencies among the strains of electrocompetent Escherichia coli cells and investigated the effect of type strain on effi ciency of transformation. Among the fi ve strains tested, Escherichia coli DH10B and TOP10 were found to be the most suitable strains for construction of genomic libraries in our laboratory. This study highlighted the fact that although these commercially available Escherichia coli strains are well characterized, they tend to differentiate and loose their transformation abilities. This may be due to the fact that the number of passages they have gone through affect their membrane structures and permeability. Therefore, every laboratory should test their strains for competency before they use them in their cloning studies

Anahtar Kelimeler

Escherichia coli transformation electrocompetency

Yıl 2008, Cilt: 2 Sayı: 2, 19 - 22, 01.07.2008

Murat Kasap Sinem Torol Gulcin Gacar

Öz

Toplam 0 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA72YC82RC
Bölüm Araştırma Makalesi
Yazarlar

Murat Kasap Bu kişi benim

Sinem Torol Bu kişi benim

Gulcin Gacar Bu kişi benim

Yayımlanma Tarihi 1 Temmuz 2008
Yayımlandığı Sayı Yıl 2008 Cilt: 2 Sayı: 2

Kaynak Göster

APA Kasap, M., Torol, S., & Gacar, G. (2008). Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. Journal of Applied Biological Sciences, 2(2), 19-22.
AMA Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. Temmuz 2008;2(2):19-22.
Chicago Kasap, Murat, Sinem Torol, ve Gulcin Gacar. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences 2, sy. 2 (Temmuz 2008): 19-22.
EndNote Kasap M, Torol S, Gacar G (01 Temmuz 2008) Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. Journal of Applied Biological Sciences 2 2 19–22.
IEEE M. Kasap, S. Torol, ve G. Gacar, “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli”, J.appl.biol.sci., c. 2, sy. 2, ss. 19–22, 2008.
ISNAD Kasap, Murat vd. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences 2/2 (Temmuz 2008), 19-22.
JAMA Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. 2008;2:19–22.
MLA Kasap, Murat vd. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences, c. 2, sy. 2, 2008, ss. 19-22.
Vancouver Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. 2008;2(2):19-22.