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Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli

Yıl 2009, Cilt: 3 Sayı: 3, 55 - 59, 01.11.2009

Öz

Trehalose, a non-reducing disaccharide is a major compatible solute, maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes encoding for trehalose biosynthesis were PCR amplified from genomic DNA of Escherichia coli isolated from Rohu (Labeo rohita) collected from local fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that (TPS) and (TPP) genes contain1,424 bp and 801 bp long ORF encoding 474 and 266 amino acids respectively. In silico sequence analysis revealed that the (TPS) and (TPP) sequences of E. coli were conserved in many eubacteria

Yıl 2009, Cilt: 3 Sayı: 3, 55 - 59, 01.11.2009

Öz

Toplam 0 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA92YG29DE
Bölüm Araştırma Makalesi
Yazarlar

- Sreevıdhya Bu kişi benim

Lawrance Anbu Rajan Bu kişi benim

Yeragudipati Rajalakshmı Bu kişi benim

Vetrivel Umashankar Bu kişi benim

Yayımlanma Tarihi 1 Kasım 2009
Yayımlandığı Sayı Yıl 2009 Cilt: 3 Sayı: 3

Kaynak Göster

APA Sreevıdhya, .-., Rajan, L. A., Rajalakshmı, Y., Umashankar, V. (2009). Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. Journal of Applied Biological Sciences, 3(3), 55-59.
AMA Sreevıdhya, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. Kasım 2009;3(3):55-59.
Chicago Sreevıdhya, -, Lawrance Anbu Rajan, Yeragudipati Rajalakshmı, ve Vetrivel Umashankar. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences 3, sy. 3 (Kasım 2009): 55-59.
EndNote Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V (01 Kasım 2009) Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. Journal of Applied Biological Sciences 3 3 55–59.
IEEE .-. Sreevıdhya, L. A. Rajan, Y. Rajalakshmı, ve V. Umashankar, “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli”, J.appl.biol.sci., c. 3, sy. 3, ss. 55–59, 2009.
ISNAD Sreevıdhya, - vd. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences 3/3 (Kasım 2009), 55-59.
JAMA Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. 2009;3:55–59.
MLA Sreevıdhya, - vd. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences, c. 3, sy. 3, 2009, ss. 55-59.
Vancouver Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. 2009;3(3):55-9.