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Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii

Yıl 2011, Cilt: 5 Sayı: 1, 7 - 10, 01.02.2011

Muthuvel Arumugam Thangappan Ajıthkumar Thangavel Balasubramanıan

Öz

Type V like collagens is widely distributed in marine invertebrates, particularly crustaceans and molluscs. The present study the collagen was prepared from the outer skin of L. uyii. The net yield of L. uyii acid soluble collagen estimated as 10.54% (ASC), subsequent digestion of residue with pepsin soluble collagen was yielded as 31.16% (PSC). SDS-PAGE analysis was revealed the chain composition of (α1)2α2 heterotrimer for ASC which similar to Japanese common squid as well as cuttle fish collagen. The ASC and PSC total protein was determined by Bradford method which contains 64.27µg/ml and 18.93µg/ml respectively. Fourier transforms infrared spectroscopy Analysis (FT-IR) of ASC and PSC is helpful in predication and confirmation of secondary structure of proteins. This report indicates that L uyii outer skin has potential to supplementing the skin of land vertebrates as a source of collagen

Anahtar Kelimeler

collagen mollusk FT-IR SDS-PAGE squid

Yıl 2011, Cilt: 5 Sayı: 1, 7 - 10, 01.02.2011

Muthuvel Arumugam Thangappan Ajıthkumar Thangavel Balasubramanıan

Öz

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Ayrıntılar

Diğer ID JA76HD33FM
Bölüm Araştırma Makalesi
Yazarlar

Muthuvel Arumugam Bu kişi benim

Thangappan Ajıthkumar Bu kişi benim

Thangavel Balasubramanıan Bu kişi benim

Yayımlanma Tarihi 1 Şubat 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 5 Sayı: 1

Kaynak Göster

APA Arumugam, M., Ajıthkumar, T., & Balasubramanıan, T. (2011). Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. Journal of Applied Biological Sciences, 5(1), 7-10.
AMA Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. Şubat 2011;5(1):7-10.
Chicago Arumugam, Muthuvel, Thangappan Ajıthkumar, ve Thangavel Balasubramanıan. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences 5, sy. 1 (Şubat 2011): 7-10.
EndNote Arumugam M, Ajıthkumar T, Balasubramanıan T (01 Şubat 2011) Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. Journal of Applied Biological Sciences 5 1 7–10.
IEEE M. Arumugam, T. Ajıthkumar, ve T. Balasubramanıan, “Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii”, J.appl.biol.sci., c. 5, sy. 1, ss. 7–10, 2011.
ISNAD Arumugam, Muthuvel vd. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences 5/1 (Şubat 2011), 7-10.
JAMA Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. 2011;5:7–10.
MLA Arumugam, Muthuvel vd. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences, c. 5, sy. 1, 2011, ss. 7-10.
Vancouver Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. 2011;5(1):7-10.