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One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960

Yıl 2011, Cilt: 5 Sayı: 2, 61 - 65, 01.07.2011

Aytac Kocabas Didem Sutay Kocabas Ufuk Bakır Bolukbası

Öz

The aim of study was to investigate purification and characterization of xylanase produced by industrially important strain of Aspergillus terreus. The xylanase was purified by one-step hydrophobic interaction chromatography technique 19-fold with 61% yield. Molecular weight and isoelectric point of the enzyme were determined as 19 kDa and pH 9.0, respectively. The enzyme was found to be unglycosylated. Kinetic experiments at 50°C and pH 7.0 resulted in apparent Km and Vmax values of 2.5±0.05 mg xylan/ml and 50.2±0.4 IU/µg protein, respectively. According to its biochemical properties, the enzyme was found to be a member of family-11 xylanase group. Due to its low molecular weight, the enzyme could be advantageous for industrial applications

Anahtar Kelimeler

Aspergillus terreus xylanase hydrophobic interaction chromatography enzyme characterization

Yıl 2011, Cilt: 5 Sayı: 2, 61 - 65, 01.07.2011

Aytac Kocabas Didem Sutay Kocabas Ufuk Bakır Bolukbası

Öz

Toplam 0 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA25GD77EN
Bölüm Araştırma Makalesi
Yazarlar

Aytac Kocabas Bu kişi benim

Didem Sutay Kocabas Bu kişi benim

Ufuk Bakır Bolukbası Bu kişi benim

Yayımlanma Tarihi 1 Temmuz 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 5 Sayı: 2

Kaynak Göster

APA Kocabas, A., Sutay Kocabas, D., & Bakır Bolukbası, U. (2011). One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. Journal of Applied Biological Sciences, 5(2), 61-65.
AMA Kocabas A, Sutay Kocabas D, Bakır Bolukbası U. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. J.appl.biol.sci. Temmuz 2011;5(2):61-65.
Chicago Kocabas, Aytac, Didem Sutay Kocabas, ve Ufuk Bakır Bolukbası. “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960”. Journal of Applied Biological Sciences 5, sy. 2 (Temmuz 2011): 61-65.
EndNote Kocabas A, Sutay Kocabas D, Bakır Bolukbası U (01 Temmuz 2011) One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. Journal of Applied Biological Sciences 5 2 61–65.
IEEE A. Kocabas, D. Sutay Kocabas, ve U. Bakır Bolukbası, “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960”, J.appl.biol.sci., c. 5, sy. 2, ss. 61–65, 2011.
ISNAD Kocabas, Aytac vd. “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960”. Journal of Applied Biological Sciences 5/2 (Temmuz 2011), 61-65.
JAMA Kocabas A, Sutay Kocabas D, Bakır Bolukbası U. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. J.appl.biol.sci. 2011;5:61–65.
MLA Kocabas, Aytac vd. “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960”. Journal of Applied Biological Sciences, c. 5, sy. 2, 2011, ss. 61-65.
Vancouver Kocabas A, Sutay Kocabas D, Bakır Bolukbası U. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. J.appl.biol.sci. 2011;5(2):61-5.