Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

M. M. Abu Teır; J. Ghıthan; S. Darwısh; M. M. Abu-hadıd

Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Year 2012, Volume: 6 Issue: 3, 45 - 55, 01.11.2012

M. M. Abu Teır J. Ghıthan S. Darwısh M. M. Abu-hadıd

Abstract

The interaction between cholesterol and human serum albumin has been investigated. The basic binding interaction was studied by UVabsorption and fluorescence spectroscopy. From spectral analysis cholesterol showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. The binding constant (k) is estimated to be K=0.214 × 104 M-1 at 293 K. FTIR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and cholesterol binding mechanisms. The observed spectral changes indicate a higher percentage of H-bonding between cholesterol and a-helix (secondary structure motif in HSA) compared to the percentage of H-bonding between cholesterol and b-sheets (secondary structure motif in HSA)

Keywords

cholesterol, amide I, amide II, amid III , binding mode, binding constant, protein secondary structure, Fourier transform IR, UV–spectroscopy, Fluorescence spectroscopy

Year 2012, Volume: 6 Issue: 3, 45 - 55, 01.11.2012

M. M. Abu Teır J. Ghıthan S. Darwısh M. M. Abu-hadıd

Abstract

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Details

Other ID	JA36AR93RR
Journal Section	Research Article
Authors	M. M. Abu Teır This is me J. Ghıthan This is me S. Darwısh This is me M. M. Abu-hadıd This is me
Publication Date	November 1, 2012
Published in Issue	Year 2012 Volume: 6 Issue: 3

Cite

APA	Teır, M. M. A., Ghıthan, J., Darwısh, S., Abu-hadıd, M. M. (2012). Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. Journal of Applied Biological Sciences, 6(3), 45-55.
AMA	Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. November 2012;6(3):45-55.
Chicago	Teır, M. M. Abu, J. Ghıthan, S. Darwısh, and M. M. Abu-hadıd. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences 6, no. 3 (November 2012): 45-55.
EndNote	Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM (November 1, 2012) Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. Journal of Applied Biological Sciences 6 3 45–55.
IEEE	M. M. A. Teır, J. Ghıthan, S. Darwısh, and M. M. Abu-hadıd, “Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin”, J.appl.biol.sci., vol. 6, no. 3, pp. 45–55, 2012.
ISNAD	Teır, M. M. Abu et al. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences 6/3 (November 2012), 45-55.
JAMA	Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. 2012;6:45–55.
MLA	Teır, M. M. Abu et al. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences, vol. 6, no. 3, 2012, pp. 45-55.
Vancouver	Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. 2012;6(3):45-5.