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Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Yıl 2012, Cilt: 6 Sayı: 3, 45 - 55, 01.11.2012

M. M. Abu Teır J. Ghıthan S. Darwısh M. M. Abu-hadıd

Öz

The interaction between cholesterol and human serum albumin has been investigated. The basic binding interaction was studied by UVabsorption and fluorescence spectroscopy. From spectral analysis cholesterol showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. The binding constant (k) is estimated to be K=0.214 × 104 M-1 at 293 K. FTIR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and cholesterol binding mechanisms. The observed spectral changes indicate a higher percentage of H-bonding between cholesterol and a-helix (secondary structure motif in HSA) compared to the percentage of H-bonding between cholesterol and b-sheets (secondary structure motif in HSA)

Anahtar Kelimeler

cholesterol amide I amide II amid III binding mode binding constant protein secondary structure Fourier transform IR UV–spectroscopy Fluorescence spectroscopy

Yıl 2012, Cilt: 6 Sayı: 3, 45 - 55, 01.11.2012

M. M. Abu Teır J. Ghıthan S. Darwısh M. M. Abu-hadıd

Öz

Toplam 0 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA36AR93RR
Bölüm Araştırma Makalesi
Yazarlar

M. M. Abu Teır Bu kişi benim

J. Ghıthan Bu kişi benim

S. Darwısh Bu kişi benim

M. M. Abu-hadıd Bu kişi benim

Yayımlanma Tarihi 1 Kasım 2012
Yayımlandığı Sayı Yıl 2012 Cilt: 6 Sayı: 3

Kaynak Göster

APA Teır, M. M. A., Ghıthan, J., Darwısh, S., Abu-hadıd, M. M. (2012). Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. Journal of Applied Biological Sciences, 6(3), 45-55.
AMA Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. Kasım 2012;6(3):45-55.
Chicago Teır, M. M. Abu, J. Ghıthan, S. Darwısh, ve M. M. Abu-hadıd. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences 6, sy. 3 (Kasım 2012): 45-55.
EndNote Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM (01 Kasım 2012) Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. Journal of Applied Biological Sciences 6 3 45–55.
IEEE M. M. A. Teır, J. Ghıthan, S. Darwısh, ve M. M. Abu-hadıd, “Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin”, J.appl.biol.sci., c. 6, sy. 3, ss. 45–55, 2012.
ISNAD Teır, M. M. Abu vd. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences 6/3 (Kasım 2012), 45-55.
JAMA Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. 2012;6:45–55.
MLA Teır, M. M. Abu vd. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences, c. 6, sy. 3, 2012, ss. 45-55.
Vancouver Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. 2012;6(3):45-5.