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Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method

Yıl 2015, Cilt: 9 Sayı: 1, 54 - 56, 01.02.2015

Fereshteh Motamedı Jalil Fallah Mehrabadı Azar Sabokbar

Öz

Background: Listeria monocytogenes is a gram positive and facultative anaerobic bacteria and Listeria species have a high spread in environment. Listeria monocytogenes is agent of meningitides, encephalitis and septicemia and could accrue during pregnancy. Detection of Listeria via culture method is difficult. The aim of this study was accessing of Listeria monocytogenes in pregnant women who refer to women hospital of Tehran via culture and Real Time PCR methods. Methods: Two hundred cervix swabs samples were collected from pregnant women in seventh and eights mounts of pregnancy and transfer to laboratory in 2ml transport medium. 1ml was used for DNA extracting directly and other keep in refrigerator for cold enrichment among 4 weeks and then cultured. Primers were designed for amplifying hly gene which coded listeriolysin O. Results: The result of culture and real time PCR methods were showed of 200 samples, 28 (14%) and 41(20.5%) were positive, respectively. Conclusion: All of 13 false negative via culture, were positive in PCR technique. So, molecular method was more sensitive and recommended for applying. Moreover, the results showed high prevalence of carrier women in this time. Therefore, public screening was recommended for pregnant women

Anahtar Kelimeler

Listeria monocytogenes culture molecular detection

Yıl 2015, Cilt: 9 Sayı: 1, 54 - 56, 01.02.2015

Fereshteh Motamedı Jalil Fallah Mehrabadı Azar Sabokbar

Öz

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Ayrıntılar

Diğer ID JA35CA45ZM
Bölüm Araştırma Makalesi
Yazarlar

Fereshteh Motamedı Bu kişi benim

Jalil Fallah Mehrabadı Bu kişi benim

Azar Sabokbar Bu kişi benim

Yayımlanma Tarihi 1 Şubat 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 9 Sayı: 1

Kaynak Göster

APA Motamedı, F., Mehrabadı, J. F., & Sabokbar, A. (2015). Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. Journal of Applied Biological Sciences, 9(1), 54-56.
AMA Motamedı F, Mehrabadı JF, Sabokbar A. Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. J.appl.biol.sci. Şubat 2015;9(1):54-56.
Chicago Motamedı, Fereshteh, Jalil Fallah Mehrabadı, ve Azar Sabokbar. “Discovery of Listeria Monocytogenes High Prevalence in Pregnant Women Who Referred to the Tehran Women Reference Hospital by Real Time PCR Method”. Journal of Applied Biological Sciences 9, sy. 1 (Şubat 2015): 54-56.
EndNote Motamedı F, Mehrabadı JF, Sabokbar A (01 Şubat 2015) Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. Journal of Applied Biological Sciences 9 1 54–56.
IEEE F. Motamedı, J. F. Mehrabadı, ve A. Sabokbar, “Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method”, J.appl.biol.sci., c. 9, sy. 1, ss. 54–56, 2015.
ISNAD Motamedı, Fereshteh vd. “Discovery of Listeria Monocytogenes High Prevalence in Pregnant Women Who Referred to the Tehran Women Reference Hospital by Real Time PCR Method”. Journal of Applied Biological Sciences 9/1 (Şubat 2015), 54-56.
JAMA Motamedı F, Mehrabadı JF, Sabokbar A. Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. J.appl.biol.sci. 2015;9:54–56.
MLA Motamedı, Fereshteh vd. “Discovery of Listeria Monocytogenes High Prevalence in Pregnant Women Who Referred to the Tehran Women Reference Hospital by Real Time PCR Method”. Journal of Applied Biological Sciences, c. 9, sy. 1, 2015, ss. 54-56.
Vancouver Motamedı F, Mehrabadı JF, Sabokbar A. Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. J.appl.biol.sci. 2015;9(1):54-6.