BibTex RIS Cite

The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme

Year 2015, Volume: 9 Issue: 1, 31 - 35, 01.02.2015

Ersin Hopa Selma Sinan Emre Sevindik

Abstract

This study aims to analyze the purification of Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oxidoreductase EC 1.1.1.49; G6PD) enzyme, which is important for metabolism, from human erythrocytes and to analyze the effects of some pesticides on enzymes. The purification process was performed in two steps being ammonium sulfate precipitation and 2’, 5’ ADP-Sepharose 4B affinity chromatography. It is identified via sodium dodecyl sulphate polyacrylamide gel (DSD-PAGE) electrophoresis that G6PD enzyme’s molecular weight is about 59000 daltons. Km values for G6PD enzyme’s NADP+ and glucose-6-phosphate (G6P) substrates are respectively 0.14mM, 0.22mM; and, Vmax values are respectively 2.76 U/mg and 1.94 U/mg. Enzyme activities were spectrophotometrically measured on 340 nm according to Beutler method. Pesticides analyzed in this study are deltamethrin and diazinon. After their specific concentration in IC50 studies, deltamethrin and diazinon substances caused turbidity in quartz vessel. Since reliable absorbance values cannot be obtained in such condition, concentration amounts of substances applied to enzyme did not reduce the activity under 50%. Because of this reason, inhibition rates could not be identified. However, it is identified that all of the pesticides show inhibition effect on enzyme activity

Keywords

G6PD İnhibition purification pesticide

Year 2015, Volume: 9 Issue: 1, 31 - 35, 01.02.2015

Ersin Hopa Selma Sinan Emre Sevindik

Abstract

There are 0 citations in total.

Details

Other ID JA97VC28PC
Journal Section Research Article
Authors

Ersin Hopa This is me

Selma Sinan This is me

Emre Sevindik This is me

Publication Date February 1, 2015
Published in Issue Year 2015 Volume: 9 Issue: 1

Cite

APA Hopa, E., Sinan, S., & Sevindik, E. (2015). The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme. Journal of Applied Biological Sciences, 9(1), 31-35.
AMA Hopa E, Sinan S, Sevindik E. The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme. J.appl.biol.sci. February 2015;9(1):31-35.
Chicago Hopa, Ersin, Selma Sinan, and Emre Sevindik. “The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme”. Journal of Applied Biological Sciences 9, no. 1 (February 2015): 31-35.
EndNote Hopa E, Sinan S, Sevindik E (February 1, 2015) The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme. Journal of Applied Biological Sciences 9 1 31–35.
IEEE E. Hopa, S. Sinan, and E. Sevindik, “The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme”, J.appl.biol.sci., vol. 9, no. 1, pp. 31–35, 2015.
ISNAD Hopa, Ersin et al. “The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme”. Journal of Applied Biological Sciences 9/1 (February 2015), 31-35.
JAMA Hopa E, Sinan S, Sevindik E. The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme. J.appl.biol.sci. 2015;9:31–35.
MLA Hopa, Ersin et al. “The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme”. Journal of Applied Biological Sciences, vol. 9, no. 1, 2015, pp. 31-35.
Vancouver Hopa E, Sinan S, Sevindik E. The Effects of Deltamethrin and Diazinon Chemicals on Human Erythrocyte Glucose-6- Phosphate Dehydrogenase Enzyme. J.appl.biol.sci. 2015;9(1):31-5.