Isolation and Molecular Characterization of Bacteria with Plant Growth-Promoting Characteristics from Magnesite Mining Fields in Kütahya-Turkey

Yıl 2016, Cilt: 10 Sayı: 3, 27 - 32, 01.11.2016

Mehmet Karadayı Medine Gulluce Selin Dogan Ekrem Gulluce

Öz

Plantation of poor soils and naturally plant growth promotion studies have dominated recent scientific trends owing to loss of productivity caused by increasing chemical pollution of soils closely associated with rising world population. In this context, Plant growth promoting bacteria (PGPB) from extreme habitats remain a key concept to develop novel alternative technologies to solve these problems. The present study was conducted to isolate potential plant growth-promoting bacteria from magnesite mining fields in Kütahya-TURKEY and identify active strains by molecular techniques. For this aim, rhizospheric soil samples for the isolation of the PGPB strains were collected from Turanocağı and Ortaocak magnesite mining fields. PGP potentials of isolates were determined by using phosphate solubilization, IAA production, siderophore production, ACC deaminase assay systems. According to the results, 10 active distinguished bacterial strains were determined as PGPBs. Data of the 16S rDNA gene sequencing showed that the active strains grouped in Arthrobacter, Bacillus, Paenibacillus and Streptomyces genera. The results of the present study suggest that different environments with stringent conditions have a valuable potential to identify new resistant PGPB strains for remediationof extreme habitats

Anahtar Kelimeler

Extreme habitat, PGPB, natural fertilizer, magnesite mine