First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır

Mesude Figen Dönmez

doi:10.56430/japro.1488826

EN

First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır

Abstract

Tomato wilt disease caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is one of the most destructive tomato diseases and causes significant crop loss in both greenhouse and field tomato production areas worldwide. In this study, the presence of the causal agent of bacterial wilt disease in tomato plants was investigated in Aras Valley. Isolation was made from diseased plant samples and it was determined whether the strains were pathogenic by cellulase activity and HR test. The virulence, morphological and biochemical characteristics of the strains were determined. Strains that fatty acid methyl ester extraction, isolation and purification were performed were identified at species and subspecies level with % similarity index using gas chromatography system. The diagnosis was confirmed with the Biolog Gen III System and all strains were identified at the subspecies level with a % similarity index. As a result of this study, 57 strains were obtained in the isolation, and 39 of the strains were determined not to be pathogenic. Strain 18 was determined as the pathogen causing the most damage to tomato plants with 100 % disease severity. Strains were identified as Cmm at subspecies level with a similarity index of 71-87 % using gas chromatography system and 54-75 % similarity index with Biolog Gen III System. According to the heat map created, it was determined that the strains consisted of two main clusters. The presence of pathogen in Aras Valley was proven for the first time by this study.

Keywords

Biolog, Clavibacter mihiganensis subsp. michiganensis, FAME, Identification, Solanum lycopersicum

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Mesude Figen Dönmez ^*
0000-0002-7992-8252
Türkiye

Publication Date

December 31, 2024

Submission Date

May 24, 2024

Acceptance Date

August 1, 2024

Published in Issue

Year 2024 Volume: 5 Number: 4

DOI

https://doi.org/10.56430/japro.1488826

IZ

https://izlik.org/JA85ZL37PX

APA

Dönmez, M. F. (2024). First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır. Journal of Agricultural Production, 5(4), 217-227. https://doi.org/10.56430/japro.1488826

AMA

1.Dönmez MF. First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır. J Agri Pro. 2024;5(4):217-227. doi:10.56430/japro.1488826

Chicago

Dönmez, Mesude Figen. 2024. “First Report of Bacterial Wilt Caused by Clavibacter Michiganensis Subsp. Michiganensis Affecting Tomato in Iğdır”. Journal of Agricultural Production 5 (4): 217-27. https://doi.org/10.56430/japro.1488826.

EndNote

Dönmez MF (December 1, 2024) First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır. Journal of Agricultural Production 5 4 217–227.

IEEE

[1]M. F. Dönmez, “First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır”, J Agri Pro, vol. 5, no. 4, pp. 217–227, Dec. 2024, doi: 10.56430/japro.1488826.

ISNAD

Dönmez, Mesude Figen. “First Report of Bacterial Wilt Caused by Clavibacter Michiganensis Subsp. Michiganensis Affecting Tomato in Iğdır”. Journal of Agricultural Production 5/4 (December 1, 2024): 217-227. https://doi.org/10.56430/japro.1488826.

JAMA

1.Dönmez MF. First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır. J Agri Pro. 2024;5:217–227.

MLA

Dönmez, Mesude Figen. “First Report of Bacterial Wilt Caused by Clavibacter Michiganensis Subsp. Michiganensis Affecting Tomato in Iğdır”. Journal of Agricultural Production, vol. 5, no. 4, Dec. 2024, pp. 217-2, doi:10.56430/japro.1488826.

Vancouver

1.Mesude Figen Dönmez. First Report of Bacterial Wilt Caused by Clavibacter michiganensis subsp. michiganensis Affecting Tomato in Iğdır. J Agri Pro. 2024 Dec. 1;5(4):217-2. doi:10.56430/japro.1488826

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