Year 2025,
Volume: 6 Issue: 4, 217 - 225, 30.12.2025
Melike Pek
,
Ertan Yıldırım
,
Melek Ekinci
References
-
Agarwal, S., & Pandey, V. (2004). Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Plantarum, 48, 555-560. https://doi.org/10.1023/B:BIOP.0000047152.07878.e7
-
Akram, N. A., Fatima, K., Kong, H., Zafar, N., Mahmood, S., Ashraf, M., & Abdel Latef, A. A. H. (2024). Interactive effect of drought stress and l-methionine on the growth and physio-biochemical changes in broccoli (Brassica oleracea L. var. italica): Leaf and head. Journal of Plant Growth Regulation, 43, 1954-1966. https://doi.org/10.1007/s00344-024-11233-x
-
Akram, N. A., Hani, U., Ashraf, M., Ashraf, M., & Sadiq, M. (2020). Exogenous application of L-methionine mitigates the drought-induced oddities in biochemical and anatomical responses of bitter gourd (Momordica charantia L.). Scientia Horticulturae, 267, 109333. https://doi.org/10.1016/j.scienta.2020.109333
-
Almas, H. I., Nisa, Z., Anwar, S., Kausar, A., Farhat, F., Munawar, M., & Khalizadieh, R. (2021). Exogenous application of methionine and phenylalanine confers salinity tolerance in tomato by concerted regulation of metabolites and antioxidants. Journal of Soil Science and Plant Nutrition, 21, 3051-3064. https://doi.org/10.1007/s42729-021-00588-9
-
Angelini, R., Manes, F., & Federico, R. (1990). Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chick-pea stems. Planta, 182(1), 89-96. https://doi.org/10.1007/bf00239989
-
El-Bauome, H. A., Abdeldaym, E. A., Abd El-Hady, M. A., Darwish, D. B. E., Alsubeie, M. S., El-Mogy, M. M., Basahi, M. A., Al-Qahtani, S. M., Al-Harbi, N. A., Alzuaibr, F. M., Alasmari, A., Ismail, I. A., Dessoky, E. S., & Doklega, S. M. (2022). Exogenous proline, methionine, and melatonin stimulate growth, quality, and drought tolerance in cauliflower plants. Agriculture, 12(9), 1301. https://doi.org/10.3390/agriculture12091301
-
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185-212. https://doi.org/10.1051/agro:2008021
-
Gong, Y., Toivonen, P. M., Wiersma, P. A., Lu, C., & Lau, O. L. (2000). Effect of freezing on the activity of catalase in apple flesh tissue. Journal of Agricultural and Food Chemistry, 48(11), 5537-5542. https://doi.org/10.1021/jf990525e
-
Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J., & Ahmad, A. (2012). Role of proline under changing environments: A review. Plant Signaling & Behavior, 7(11), 1456-1466. https://doi.org/10.4161/psb.21949
-
Iqbal, B., Hussain, F., Khan, M. S., Iqbal, T., Shah, W., Ali, B., Al Syaad, K. M., & Ercisli, S. (2023). Physiology of gamma-aminobutyric acid treated Capsicum annuum L. (Sweet pepper) under induced drought stress. PLOS One, 18(8), e0289900. https://doi.org/10.1371/journal.pone.0289900
-
Kantar, M. B., Anderson, J. E., Lucht, S. A., Mercer, K., Bernau, V., Case, K. A., Le, N. C., Frederiksen, M. K., DeKeyser, H. C., Wong, Z. Z., Hastings, J. C., & Baumler, D. J. (2016). Vitamin variation in Capsicum spp. provides opportunities to improve nutritional value of human diets. PLOS One, 11(8), e0161464. https://doi.org/10.1371/journal.pone.0161464
-
Kuşvuran, Ş., Kıran, S. U., & Altuntaş, Ö. (2020). Farklı biber genotiplerinde kuraklığın morfolojik, fizyolojik ve biyokimyasal etkileri. Turkish Journal of Agriculture-Food Science and Technology, 8(6), 1359-1368. https://doi.org/10.24925/turjaf.v8i6.1359-1368.3375 (In Turkish)
-
Lu, X., Wu, Q., Nie, K., Wu, H., Chen, G., Wang, J., & Ma, Z. (2023). Exogenous phthalanilic acid induces resistance to drought stress in pepper seedlings (Capsicum annuum L.). Frontiers in Plant Science, 14, 1156276. https://doi.org/10.3389/fpls.2023.1156276
-
Maqsood, M. F., Shahbaz, M., Kanwal, S., Kaleem, M., Shah, S. M. R., Luqman, M., Iftikhar, I., Zulfikar, U., Tariq, A., Naveed, S. A., Inayat, N., Ud Din, M. A., Uzair, M., Khan, M. R., & Farhat, F. (2022). Methionine promotes the growth and yield of wheat under water deficit conditions by regulating the antioxidant enzymes, reactive oxygen species, and ions. Life, 12(7), 969. https://doi.org/10.3390/life12070969
-
Okunlola, G. O., Olatunji, O. A., Akinwale, R. O., Tariq, A., & Adelusi, A. A. (2017). Physiological response of the three most cultivated pepper species (Capsicum spp.) in Africa to drought stress imposed at three stages of growth and development. Scientia Horticulturae, 224, 198-205. https://doi.org/10.1016/j.scienta.2017.06.020
-
Pandey, S. K., Yadav, S. K., & Singh, V. K. (2012). An overview on Capsicum annuum L. Journal of Pharmaceutical Science and Technology, 4(2), 821-828.
-
Shams, M., Ekinci, M., Ors, S., Turan, M., Agar, G., Kul, R., & Yildirim, E. (2019). Nitric oxide mitigates salt stress effects of pepper seedlings by altering nutrient uptake, enzyme activity and osmolyte accumulation. Physiology and Molecular Biology of Plants, 25(5), 1149-1161. https://doi.org/10.1007/s12298-019-00692-2
-
Witham, F. H., Blaydes, D. F., & Devlin, R. M. (1971). Experiments in plant physiology. Van Nostrand Reinhold Company.
-
Yaldız, G., & Özgüven, M. (2011). Farklı süs biberi (Capsicum Sp.) tür ve hatlarının Çukurova koşullarına adaptasyonu. Yuzuncu Yıl University Journal of Agricultural Sciences, 21(1), 1-11. (In Turkish)
-
Ye, Y., Tam, N. F. Y., Wong, Y. S., & Lu, C. Y. (2003). Growth and physiological responses of two mangrove species (Bruguiera gymnorrhiza and Kandelia candel) to waterlogging. Environmental and Experimental Botany, 49(3), 209-221. https://doi.org/10.1016/S0098-8472(02)00071-0
-
Yordanova, R. Y., Christov, K. N., & Popova, L. P. (2004). Antioxidative enzymes in barley plants subjected to soil flooding. Environmental and Experimental Botany, 51(2), 93-101. https://doi.org/10.1016/S0098-8472(03)00063-7
-
Zhang, X., Ma, X., Wang, S., Liu, S., & Shi, S. (2024). Physiological and genetic aspects of resistance to abiotic stresses in Capsicum species. Plants, 13(21), 3013. https://doi.org/10.3390/plants13213013
Alleviating Effect of Exogenous Methionine on Growth and Antioxidant Defense of Mechanisms Pepper Seedlings Under Drought Stress
Year 2025,
Volume: 6 Issue: 4, 217 - 225, 30.12.2025
Melike Pek
,
Ertan Yıldırım
,
Melek Ekinci
Abstract
This study aimed to reduce the negative effects of drought stress on pepper seedlings by methionine application. In the experiment, which was organized according to the randomized plots experimental design as a pot study, two different irrigation levels (100% and 60% field capacity) and different methionine (M0: 0 mg/L (control), M10: 10 mg/L and M20: 20 mg/L) doses were applied to pepper (Capsicum annuum L.) seedlings. In the study, the effects of the applications on morphological parameters, chlorophyll content, antioxidant enzyme activities, and stress markers were evaluated. According to the findings, drought stress caused negative effects on plant development in pepper seedlings. However, methionine applications alleviated these effects. Although the effectiveness varied according to the methionine application dose, it was determined that both doses gave positive results in different parameters. In drought conditions, methionine applications increased plant growth and antioxidant enzyme levels and strengthened the plant's defense responses. As a result, it was determined that the negativities caused by water restriction in pepper seedlings were alleviated by methionine application and thus provided positive contributions at the morphological and physiological levels in pepper seedlings.
Ethical Statement
This study does not require ethical committee approval.
Thanks
This study includes the results of Melike PEK's master's thesis.
References
-
Agarwal, S., & Pandey, V. (2004). Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Plantarum, 48, 555-560. https://doi.org/10.1023/B:BIOP.0000047152.07878.e7
-
Akram, N. A., Fatima, K., Kong, H., Zafar, N., Mahmood, S., Ashraf, M., & Abdel Latef, A. A. H. (2024). Interactive effect of drought stress and l-methionine on the growth and physio-biochemical changes in broccoli (Brassica oleracea L. var. italica): Leaf and head. Journal of Plant Growth Regulation, 43, 1954-1966. https://doi.org/10.1007/s00344-024-11233-x
-
Akram, N. A., Hani, U., Ashraf, M., Ashraf, M., & Sadiq, M. (2020). Exogenous application of L-methionine mitigates the drought-induced oddities in biochemical and anatomical responses of bitter gourd (Momordica charantia L.). Scientia Horticulturae, 267, 109333. https://doi.org/10.1016/j.scienta.2020.109333
-
Almas, H. I., Nisa, Z., Anwar, S., Kausar, A., Farhat, F., Munawar, M., & Khalizadieh, R. (2021). Exogenous application of methionine and phenylalanine confers salinity tolerance in tomato by concerted regulation of metabolites and antioxidants. Journal of Soil Science and Plant Nutrition, 21, 3051-3064. https://doi.org/10.1007/s42729-021-00588-9
-
Angelini, R., Manes, F., & Federico, R. (1990). Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chick-pea stems. Planta, 182(1), 89-96. https://doi.org/10.1007/bf00239989
-
El-Bauome, H. A., Abdeldaym, E. A., Abd El-Hady, M. A., Darwish, D. B. E., Alsubeie, M. S., El-Mogy, M. M., Basahi, M. A., Al-Qahtani, S. M., Al-Harbi, N. A., Alzuaibr, F. M., Alasmari, A., Ismail, I. A., Dessoky, E. S., & Doklega, S. M. (2022). Exogenous proline, methionine, and melatonin stimulate growth, quality, and drought tolerance in cauliflower plants. Agriculture, 12(9), 1301. https://doi.org/10.3390/agriculture12091301
-
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185-212. https://doi.org/10.1051/agro:2008021
-
Gong, Y., Toivonen, P. M., Wiersma, P. A., Lu, C., & Lau, O. L. (2000). Effect of freezing on the activity of catalase in apple flesh tissue. Journal of Agricultural and Food Chemistry, 48(11), 5537-5542. https://doi.org/10.1021/jf990525e
-
Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J., & Ahmad, A. (2012). Role of proline under changing environments: A review. Plant Signaling & Behavior, 7(11), 1456-1466. https://doi.org/10.4161/psb.21949
-
Iqbal, B., Hussain, F., Khan, M. S., Iqbal, T., Shah, W., Ali, B., Al Syaad, K. M., & Ercisli, S. (2023). Physiology of gamma-aminobutyric acid treated Capsicum annuum L. (Sweet pepper) under induced drought stress. PLOS One, 18(8), e0289900. https://doi.org/10.1371/journal.pone.0289900
-
Kantar, M. B., Anderson, J. E., Lucht, S. A., Mercer, K., Bernau, V., Case, K. A., Le, N. C., Frederiksen, M. K., DeKeyser, H. C., Wong, Z. Z., Hastings, J. C., & Baumler, D. J. (2016). Vitamin variation in Capsicum spp. provides opportunities to improve nutritional value of human diets. PLOS One, 11(8), e0161464. https://doi.org/10.1371/journal.pone.0161464
-
Kuşvuran, Ş., Kıran, S. U., & Altuntaş, Ö. (2020). Farklı biber genotiplerinde kuraklığın morfolojik, fizyolojik ve biyokimyasal etkileri. Turkish Journal of Agriculture-Food Science and Technology, 8(6), 1359-1368. https://doi.org/10.24925/turjaf.v8i6.1359-1368.3375 (In Turkish)
-
Lu, X., Wu, Q., Nie, K., Wu, H., Chen, G., Wang, J., & Ma, Z. (2023). Exogenous phthalanilic acid induces resistance to drought stress in pepper seedlings (Capsicum annuum L.). Frontiers in Plant Science, 14, 1156276. https://doi.org/10.3389/fpls.2023.1156276
-
Maqsood, M. F., Shahbaz, M., Kanwal, S., Kaleem, M., Shah, S. M. R., Luqman, M., Iftikhar, I., Zulfikar, U., Tariq, A., Naveed, S. A., Inayat, N., Ud Din, M. A., Uzair, M., Khan, M. R., & Farhat, F. (2022). Methionine promotes the growth and yield of wheat under water deficit conditions by regulating the antioxidant enzymes, reactive oxygen species, and ions. Life, 12(7), 969. https://doi.org/10.3390/life12070969
-
Okunlola, G. O., Olatunji, O. A., Akinwale, R. O., Tariq, A., & Adelusi, A. A. (2017). Physiological response of the three most cultivated pepper species (Capsicum spp.) in Africa to drought stress imposed at three stages of growth and development. Scientia Horticulturae, 224, 198-205. https://doi.org/10.1016/j.scienta.2017.06.020
-
Pandey, S. K., Yadav, S. K., & Singh, V. K. (2012). An overview on Capsicum annuum L. Journal of Pharmaceutical Science and Technology, 4(2), 821-828.
-
Shams, M., Ekinci, M., Ors, S., Turan, M., Agar, G., Kul, R., & Yildirim, E. (2019). Nitric oxide mitigates salt stress effects of pepper seedlings by altering nutrient uptake, enzyme activity and osmolyte accumulation. Physiology and Molecular Biology of Plants, 25(5), 1149-1161. https://doi.org/10.1007/s12298-019-00692-2
-
Witham, F. H., Blaydes, D. F., & Devlin, R. M. (1971). Experiments in plant physiology. Van Nostrand Reinhold Company.
-
Yaldız, G., & Özgüven, M. (2011). Farklı süs biberi (Capsicum Sp.) tür ve hatlarının Çukurova koşullarına adaptasyonu. Yuzuncu Yıl University Journal of Agricultural Sciences, 21(1), 1-11. (In Turkish)
-
Ye, Y., Tam, N. F. Y., Wong, Y. S., & Lu, C. Y. (2003). Growth and physiological responses of two mangrove species (Bruguiera gymnorrhiza and Kandelia candel) to waterlogging. Environmental and Experimental Botany, 49(3), 209-221. https://doi.org/10.1016/S0098-8472(02)00071-0
-
Yordanova, R. Y., Christov, K. N., & Popova, L. P. (2004). Antioxidative enzymes in barley plants subjected to soil flooding. Environmental and Experimental Botany, 51(2), 93-101. https://doi.org/10.1016/S0098-8472(03)00063-7
-
Zhang, X., Ma, X., Wang, S., Liu, S., & Shi, S. (2024). Physiological and genetic aspects of resistance to abiotic stresses in Capsicum species. Plants, 13(21), 3013. https://doi.org/10.3390/plants13213013