Chemical Composition, Antibacterial and Antioxidant Activity of Essential Oils and Extracts of Ferula orientalis

Yıl 2023, Cilt: 4 Sayı: 2, 159 - 168, 30.12.2023

Esin Dadaşoğlu , Nasibe Tekiner , Aykut Öztekin

https://doi.org/10.56430/japro.1401560

Öz

This study aimed to determine the essential oil content, essential oil and extracts, which are known as Ferula orientalis and obtained from naturally grown plants in Narman (Erzurum, Türkiye) province, antioxidant effects and antimicrobial effects. F. orientalis essential oils were isolated by hydrodistillation and analyzed using gas chromatography-mass spectrometry to identify their components. The antimicrobial activity was measured by the disc diffusion methods and minimal inhibitory concentration (MIC) methods against Chryseobacterium indologenes which cause soft rot in certain vegetables and fruits. Total antioxidant and phenolic contents were analyzed by 2,2′- diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6 sulphonic acid (ABTS), thiobarbituric acid reactive species (TBARS), β-carotene bleaching test (BCB) and Folin-Ciocalteu method. According to the results obtained; F. orientalis essential oil exhibited a high content of δ-3-Carene (40.38%) as major compound over 14 identified components by GC-MS analysis followed by γ-Terpinene (17.24%), (E)-β-Ocimene (10.51%), and β-Phellandrene (8.49%). The essential oil and extracts was evaluated for its antimicrobial activity against C. indologenes showed significant antibacterial activities with MIC values of 9-21 mm and 62.5 μg/mL, respectively, but extracts and antibiotics have no effect against C. indologenes. Hexane extract had the highest ABTS free radical scavenging activity with 14.2 (IC50 g/l), acetone extract had the highest DPPH capacity with 24.2 (IC50 g/l), and water extract had the highest amount of total phenolic compound with 15.13±3.82 mg GAE/g. In the TBARS test antioxidant activity increased as the amount of essential oil increased. The antioxidant capacity of F. orientalis essential oil exhibited reduction when evaluated by β-carotene bleaching assay. As a result, it is thought that F. orientalis essential oils and extracts can be used as an alternative natural antioxidant source for potential applications.

Anahtar Kelimeler

Antibacterial activity, Antioxidant activity, Chryseobacterium indologenes, Essential oils, Ferula orientalis

Etik Beyan

no

Destekleyen Kurum

No

Teşekkür

mo

Kaynakça

• Abu-Shanab, B., Adwan, G., Abu-Shanab, D., Jarrar, N., & Adwan, K. (2004). Antibacterial activities of some plant extracts utilized in popular medicine in Palestine. Turkish Journal of Biology, 28(2), 99-102.
• Adams, R. P. (2017). Identification of essential oil components by gas chromatography/mass spectrometry. Gruver.
• Adebolu, T. T., & Oladimeji, A. S. (2005). Antimicrobial activity of leaf exracts of Ocimum gratissimum on selected diarrhoea causing bacrteria in southwestern Nigeria. African Journal of Biotechnology, 4(7), 682-684. https://doi.org/10.5897/AJB2005.000-3126
• Adıgüzel, A., Güllüce, M., Şengul, M., Öğütçü, H., Sahin, F., & Karaman, I. (2005). Antimicrobial effect of Ocimum basilicum (Labiatae) exract. Turkish Journal of Biology, 29, 155-160.
• Agrios, G. N., (2005). Plant pathology. Department of Plant Pathology, University of Florida, Elsevier Academic Press.
• Al-Ja’fari, A. H., Vila, R., & Freixa, B. (2013). Antifungal compounds from the rhizome and roots of Ferula hermonis. Phytotherapy Research, 27(6), 911-915. https://doi.org/10.1002/ptr.4806
• Celiktas, O. Y., Kocabas, E. E. H., Bedir, E., Sukan, F. V., Özek, T., & Baser, K. H. C. (2007). Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry, 100, 553-559. https://doi.org/10.1016/j.foodchem.2005.10.011
• Dadaşoğlu, F. (2013). Artvin, Erzincan, Erzurum, Iğdır illerinde bazı meyve ve sebzelerde yumuşak çürüklüğe sebep olan bakterilerin izolasyonu klasik ve moleküler yöntemler ile karakterizasyonu (Doctoral dissertation, Atatürk University). (In Turkish)
• Dadasoglu, F., Kotan, R., Cakır, A., Cakmakcı, R., Kordalı, S., & Ozer, H. (2015). Antibacterial activities of essential oils, extracts and some of their major components of Artemisia spp. against seed-borne plant pathogenic bacteria. Fresenius Environmental Bulletin, 24, 2715-2724.
• Dadasoglu, F., Kotan, R., Cakır, A., Karagoz, K., Dikbas, N., & Ozer, H. (2016). Use of essential oils and extracts from Satureja and Origanum species as seed disinfectants against Xanthomonas axonopodis pv. vesicatoria (Doidge) Dye. Fresenius Environmental Bulletin, 25(1A), 5989-5998.
• Dadasoglu, E., Oztekin, A., & Dadasoglu, F. (2018). Antibacterial and antioxidant activity of essential oil and extracts of Ferula communis and determination of chemical composition of its essential oil. Fresenius Environmental Bulletin, 27(6), 4186-4191.
• Gomes, M. S., Cardoso, M. G., Soares, M. J., Batista, L. R., Machado, S. M. F., Andrade, M. A., Azeredo, C. M. O., Resende, J. M. V., & Rodrigues, L. M. A. (2014). Use of essential oils of the genus citrus as biocidal agents. American Journal of Plant Sciences, 5, 299-305. https://doi.org/10.4236/ajps.2014.53041
• Görmez, A., Bozarı, S., Yanmış, D., Güllüce, M., Şahin, F., & Ağar, G. (2015). Chemical composition and antibacterial activity of essential oils of two species of Lamiaceae against phytopathogenic bacteria. Polish Journal of Microbiology, 64(2), 121-127.
• Iranshahy, M., & Iranshahi, M. (2011). Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gumresin)-A review. Journal of Ethnopharmacology, 134, 1-10. https://doi.org/10.1016/j.jep.2010.11.067
• Iroegbu, C. U., & Nkere, C. K. (2005). Evalution of the antibacterial properties of Picralima nitida stembark extracts. International Journal of Molecular Medicine and Advance Sciences, 1(2), 182-189.
• Jennings, W., & Shibamoto, J. (1980). Qualitative analysis of flavor and fragrance volatiles by capillary gas chromatography. Academic Press.
• Kartal, N., Sokmen, M., Tepe, B., Daferera, D., Polissiou, M., & Sokmen, A. (2007). Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food Chemistry, 100(2), 584-589. https://doi.org/10.1016/j.foodchem.2005.09.084
• Kotan, R., Cakir, A., Dadasoglu, F., Aydin, T., Cakmakci, R., Ozer, H., Kordali, S., Mete, E., & Dikbas, N. (2010). Antibacterial activities of essential oils and extracts of Turkish Achillea, Satureja and Thymus species against plant pathogenic bacteria. Journal of the Science of Food and Agriculture, 90, 145-160. https://doi.org/10.1002/jsfa.3799
• Kotan, R., Cakir, A., Ozer, H., Kordali, S., Cakmakci, R., Dadasoglu, F., Dikbas, N., Aydin, T., & Kazaz, C. (2014). Antibacterial effects of Origanum onites against phytopathogenic bacteria: Possible use of the extracts from protection of disease caused by some phytopathogenic bacteria. Horticultural Science, 172, 210-220. https://doi.org/10.1016/j.scienta.2014.03.016
• Kulisic, T., Radonicb, A., Katalinicc, V., & Milos, M. (2004). Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry, 85(4), 633-640. https://doi.org/10.1016/j.foodchem.2003.07.024
• Maggi, F., Papa, F., Dall’Acqua, S., & Nicoletti, M. (2016). Chemical analysis of essential oils from different parts of Ferula orientalis L. growing in central Italy. Natural Product Research, 30, 806-813. https://doi.org/10.1080/14786419.2015.1071365
• Mahendra, P., & Bisht, S. (2012). Ferula asafoetida: Traditional uses and pharmacological activity. Pharmacognosy Reviews, 6(12), 141. https://doi.org/10.4103%2F0973-7847.99948
• Mallett, J. F., Cerrati, C., Ucciani, E., Gamisana, J., & Gruber, M. (1994). Antioxidant activity of plant leaves in relation to their α-tocopherol content. Food Chemistry, 49, 61-65. https://doi.org/10.1016/0308-8146(94)90233-X
• Meral, G., & Karabay, N. U. (2002). In vitro antibacterial activities of three Hypericum species from west Anatolia. Electronic Journal of Biotechnology, 19, 6-10.
• Miranda, C. A. S. F., Cardoso, M. G., Machado, S. M. F., Gomes, M. S., Andrade, J., & Teixeira, M. L. (2014). Correlacao entre composicao quimica e eficacia antioxidante de oleos essenciais de plantas condimentares por. E-xacta, 7(1), 65-74. https://doi.org/10.18674/exacta.v7i1.1223 (In Portuguese)
• Mohammadhosseini, M., Venditti, A., Sarker, S. D., Nahar, L., & Akbarzadeh, A. (2019). The genus Ferula: Ethnobotany, phytochemistry and bioactivities-A review. Industrial Crops Production, 129, 350-394. https://doi.org/10.1016/j.indcrop.2018.12.012
• Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C., & Yolke, R. H. (1995). Manual of clinical microbiology. Amer Society For Microbiology.
• Nguir, A., Mabrouk, H., Douki, W., Ismail, M. B., Jannet, H. B., Flamini, G., & Hamza, M. A. (2016). Chemical composition and bioactivities of the essential oil from different organs of Ferula orientalis L. growing in Tunisia. Medicinal Chemistry Research, 25, 515-525. https://doi.org/10.1007/s00044-016-1506-1
• Okeke, M. I., Iroegbu, C. U., Eze, A. S., Okoli, A. S., & Esimone, C. O. (2001). Evaluation of exract of root of Landolphia owerrience for antibacterial activity. Journal of Ethnopharmacology, 78, 119-127. https://doi.org/10.1016/S0378-8741(01)00307-5
• Ozkan, H., Yanmıs, D., Karadayı, M., Bal, T., Barıs, O., & Gulluce, M. (2014). Determination of genotoxic and antigenotoxic properties of essential oil from Ferula orientalis L. using Ames/Salmonella and E. coli WP2 bacterial test systems. Toxicology and Industrial Health, 30(8), 714-723. https://doi.org/10.1177/0748233712462479
• Pavlovic, I., Petrovic, S., Radenkovic, M., Milenkovic, M., Couladis, M., Brankovic, S., Drobac, M. P., & Niketic, M. (2012). Composition, antimicrobial, antiradical and spasmolytic activity of Ferula heuffelii Griseb. ex Heuffel (Apiaceae) essential oil. Food Chemistry, 130(2), 310-315. https://doi.org/10.1016/j.foodchem.2011.07.043
• Perombelon, M. C., & Kelman, A. (1980). Ecology of the soft rot erwinias. Annual Review of Phytopathology, 18(1), 361-387. https://doi.org/10.1146/annurev.py.18.090180.002045
• POWO (Plants of the World Online). (2002). Ferula L. Retrieved Aug 30, 2022, from https://powo.science.kew.org/taxon/30105171-2#publications
• Rodriguez, H., Aguılar, L., & Lao, M. (1997). Variations in xanthan production by antibiotic-resistant mutants of Xanthomonas campestris. Applied Microbiology and Biotechnology, 48, 626-629. https://doi.org/10.1007/s002530051106
• Rojas, J. J., Veronica, J. O., Ocampo, S. A., & Munoz, J. F. (2006). Screening for antimicrobial activity of ten medicinal plant used in Colombian folkloric medicine: A possible alternative in THA treatment of non-nosocomial infections. BMC Complementary Medicine and Therapies, 6, 2. https://doi.org/10.1186/1472-6882-6-2
• Ruberto, G., & Baratta, M. T. (2000). Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69(2), 167-174. https://doi.org/10.1016/S0308-8146(99)00247-2
• Sahebkar, A., & Iranshahi, M. (2013). Volatile constituents of the genus Ferula (Apiaceae): A review. Journal of Essential Oil Bearing Plants, 14(5), 504-531. https://doi.org/10.1080/0972060X.2011.10643969
• Sahin, F., & Miller, S. A. (1997). Identification of the bacterial leaf spot pathogen of lettuce, Xanthomonas campestris pv. vitians, in Ohio, and assessment of cultivar resistance and seed treatment. Plant Disease, 81,1443-1446. https://doi.org/10.1094/PDIS.1997.81.12.1443
• Sahin, F., Karaman, I., Gulluce, M., Ogutcu, H., Sengul, M., Adıguzel, A., Öztürk, S., & Kotan, R. (2003). Evalation of antimicrobial actives of Satureja hortensis L. Journal of Ethnopharmacology, 87, 61-65. https://doi.org/10.1016/S0378-8741(03)00110-7
• Schlesier, K., Harwat, M., Bohm, V., & Bitsch, R. (2002). Assessment of antioxidant activity by using different in vitro methods. Free Radical Research, 36, 177e187. https://doi.org/10.1080/10715760290006411
• Sechi, L. A., Lezcano, I., Nunez, N., Espim, M., Duprè, I., Pinna, A., Molicotti, P., Fadda, G., & Zanetti, S. (2001). Antibacterial activity of ozonized sunflower oil (oleozon). Journal of Applied Microbiology, 90, 279-284. https://doi.org/10.1046/j.1365-2672.2001.01235.x
• Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40, 945-948. https://doi.org/10.1021/jf00018a005
• Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
• Tepe, B., Sokmen, M., Akpulat, A. H., & Sokmen, A. (2006). Screening of THA antioxidant potentials of six Salvia species from Turkey. Food Chemistry, 95, 200-204. https://doi.org/10.1016/j.foodchem.2004.12.031
• Topdas, E. F., Sengul, M., Taghizadehghalehjoughi, A., & Hacımuftuoglu, A. (2020). Neuroprotective potential and antioxidant activity of various solvent extracts and essential oil of Ferula orientalis L. Journal of Essential Oil Bearing Plants, 23(1), 121-138. https://doi.org/10.1080/0972060X.2020.1729247
• Tubives. (2019). Turkish Plants Data Service. Retrieved Jun 18, 2019, from http://194.27.225.161/yasin/tubives/index.php?sayfa=1&tax_id=4347
• White, D. G., Zhao, S., Simjee, S., Wagner, D. D., & Mcdermott, P. F. (2002). Antimicrobial resistance of food-borne pathogens. Microbes and Infection, 4, 405-412. https://doi.org/10.1016/S1286-4579(02)01554-X
• Wu, L., Chang, L., Chen, S., Fan, N., & Ho, J. A. (2009). Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. Food Science and Technology, 42, 1513-1519. https://doi.org/10.1016/j.lwt.2009.04.004
• Yegen, O., Basım, H., Laux, P., & Zeller, W. (2002). Biocontrol of Erwinia amylovora with a natural product. Iobc Wprs Bulletin, 25(10), 147-150.