More Abundant Superoxide Dismutase2 Protein Levels in Blood May Act as a Prognostic Marker for High-Risk Neuroblastoma Patients

Abstract

Purpose: Determination of proteomic differences plays an important role in biomarker investigations. Due to its heterogenic molecular background, identification of certain biomarkers is still a demand both for diagnosis and for prognosis of neuroblastoma. In this study, it is aimed to identify some marker proteins/mechanisms that may play role in neuroblastoma prognosis. Methods: A proteomic approach was performed for different risk groups of the disease by using matrix-assisted laser desorption ionization–time of flight (MALDI-TOF/TOF) approach. Mononuclear cell pools from blood samples of patients for risk groups were constructed and protein expression changes for different groups were identified. Real-time PCR analysis were performed for N-MYC, 11q, 1p and 17q status of these patients and risk groups were determined from tumor samples. Results: Manganese-superoxide dismutase (SOD2) protein was significantly increased in high-risk group of neuroblastoma patients. Conclusion: SOD2 may play an important role in neuroblastoma progression and be a candidate prognostic peripheral blood marker for neuroblastoma patients.

Keywords

• Neuroblastoma
• SOD2
• Risk-classification
• Proteomics
• MALDI-TOF/TOF

Supporting Institution

This study has been supported by a research grant of Turkish Pediatric Oncology Group (TPOG)

Project Number

2015

Thanks

This study has been supported by a research grant of Turkish Pediatric Oncology Group (TPOG) (2015) and performed on behalf of TPOG. The study has been presented at 60th Annual Meeting of the Japanese Society of Pediatric Hematology Society as a poster. 2D Gel Electrophoresis and MALDI TOF/TOF analysis were performed in Prof. Dr. Talat YALCIN’s laboratory in Izmir Institute of Technology, Department of Chemistry.

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