Effects of Adiponectin on Akt Expression Under Hypoglycemic and Hyperglycemic Conditions in HL-1 Cardiomyocytes

Abstract

Background purpose: Diabetic cardiomyopathy is a distinct cardiac disorder that develops independently of conventional risk factors such as coronary artery disease and hypertension. Adiponectin activates various intracellular signaling cascades. Akt, a critical serine/threonine kinase in cardiomyocytes, regulates cell survival, growth and metabolism. The role of Adiponectin in regulating Akt in Diabetic cardiomyopahty remains unclear. This study aimed to investigate how Adiponectin regulates Akt expression under varying glucose conditions mimicking hypoglycemic and hyperglycemic conditions in the HL-1 cardiac cell line. Methods: Total RNA was isolated from HL-1 cells and Akt gene expression was analyzed by RT-PCR using specific forward and reverse primers. Statistical analysis was performed using two-way ANOVA followed by Tukey’s multiple comparison test. Results: Adiponectin showed differential effects on Akt expression depending on glucose concentration, as well as dose and time dependent conditions in HL-1 cells. Conclusion: Adiponectin stimulation significantly regulated Akt expression under hypoglycemic, basal, and hyperglycemic conditions. These findings suggest that Adiponectin regulates the insulin signaling pathway and may influence cardiac energy metabolism by shifting substrate utilization toward fatty acids. Therefore, Adiponectin may represent a potential therapeutic target in Diabetic cardiomyopathy and contribute novel insights to the literature.

Keywords

• Adiponectin
• Akt
• Diabetic cardiomyopathy
• HL-1 cells

Supporting Institution

Health Institutes of Türkiye (project number: 2022-B-03 27862).

Project Number

Health Institutes of Türkiye project number: 2022-B-03 27862

Ethical Statement

Ethical approval for this study was obtained from the Dokuz Eylül University Ethics Committee(2023/07-14)

Thanks

We thank Health Institutes of Türkiye for their financial support for project number: 2022-B-03 27862.

Effects of Adiponectin on Akt Expression Under Hypoglycemic and Hyperglycemic Conditions in HL-1 Cardiomyocytes

Abstract

Amaç: Diyabetik kardiyomiyopati, koroner arter hastalığı ve hipertansiyon gibi geleneksel risk faktörlerinden bağımsız olarak gelişen özgün bir kalp hastalığıdır. Adiponektin, çeşitli hücre içi sinyal yolaklarını aktive eder. Kardiyomiyositlerde kritik bir serin/treonin kinaz olan Akt, hücre sağkalımı, büyüme ve metabolizmayı düzenler. Diyabetik kardiyomiyopatide adiponektinin Akt’ı düzenlemedeki rolü ise henüz tam olarak aydınlatılmamıştır. Bu çalışma, HL-1 kalp hücre hattında hipoglisemik ve hiperglisemik koşulları taklit eden farklı glukoz düzeyleri altında adiponektinin Akt ekspresyonunu nasıl düzenlediğini araştırmayı amaçlamıştır. Yöntemler: HL-1 hücrelerinden total RNA izole edildi ve Akt gen ekspresyonu, spesifik forward ve reverse primerler kullanılarak RT-PCR ile analiz edildi. İstatistiksel analizler, iki yönlü ANOVA ve ardından Tukey çoklu karşılaştırma testi ile gerçekleştirildi. Bulgular: Adiponektinin, HL-1 hücrelerinde Akt ekspresyonu üzerinde glukoz konsantrasyonuna bağlı olarak farklı etkiler gösterdiği; ayrıca doz ve zamana bağlı değişimler sergilediği gözlendi. Sonuç: Adiponektin uyarımı, hipoglisemik, bazal ve hiperglisemik koşullar altında Akt ekspresyonunu anlamlı şekilde düzenlemiştir. Bu bulgular, adiponektinin insülin sinyal yolunu düzenlediğini ve substrat kullanımını yağ asitlerine kaydırarak kardiyak enerji metabolizmasını etkileyebileceğini düşündürmektedir. Bu nedenle adiponektin, diyabetik kardiyomiyopatide potansiyel bir terapötik hedef olabilir ve literatüre yeni katkılar sunabilir.

Keywords

• Adiponektin
• Akt
• Diyabetik kardiyomiyopati
• HL-1 Hücreleri

Supporting Institution

Türkiye Sağlık Enstitüleri Başkanlığı tarafından desteklenmiştir (proje no: 2022-B-03 27862).

Project Number

Health Institutes of Türkiye project number: 2022-B-03 27862

Ethical Statement

Dokuz Eylül Üniversitesi Girişimsel Olmayan Araştırmalar Etik Kurulu karar no: 2023/07-14, etik açıdan uygun olduğu değerlendirilmiştir.

Thanks

Finansal destekleri için Türkiye Sağlık Enstitüleri Başkanlığına teşekkür ederiz (proje no: 2022-B-03 27862)

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