Concentration-Dependent Inhibitory Effect Of Calcium Fructoborate On Sirt1 Enzyme Activity: A Comparative Analysis With Boric Acid

Abstract

Amaç: SIRT1; hücresel yaşlanma, apoptoz, glukoz ve lipid metabolizması, oksidatif stres ve inflamasyon gibi düzenleyici mekanizmalarda görev almaktadır. Çalışmalar, SIRT1 inhibitörlerinin çeşitli kanser türleri ve metabolik bozukluklar üzerinde etkili olabileceğini göstermekte ve bu durumu SIRT1’i önemli bir terapötik hedef haline getirmektedir. Borik asit; antimikrobiyal, antifungal ve potansiyel antikanser etkileri bulunan inorganik bir bor bileşiğidir. Sebze ve meyvelerde bulunan diğer bir bor bileşiği olan kalsiyum fruktoborat ise antiinflamatuvar, antioksidan ve antitümör etkilere sahiptir. Bu çalışmada, kalsiyum fruktoboratın SIRT1 aktivitesi üzerindeki inhibitör potansiyelinin araştırılması ve gelecekte SIRT1 regülasyonunu hedefleyen çalışmalarda kimyasal araç olarak olası kullanımının değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada, kalsiyum fruktoboratın SIRT1 aktivitesi üzerindeki etkisi, insan rekombinant SIRT1 kullanılarak floresans temelli bir yöntem ile araştırılmıştır. Bulgular: Kalsiyum fruktoborat, SIRT1 aktivitesini doza bağlı şekilde inhibe etmiştir. 20 mM konsantrasyonda kalsiyum fruktoborat, SIRT1 aktivitesini anlamlı olarak %42 oranında inhibe etmiştir (p < 0.001). Borik asit grubunda ise SIRT1 enzim aktivitesinde azalma eğilimi gözlenmiş, ancak bu fark istatistiksel olarak anlamlı bulunmamıştır. Sonuç: Elde edilen sonuçlar önemli temel bilgiler sunmaktadır. Kalsiyum fruktoborat, SIRT1’in biyolojik fonksiyonlarının aydınlatılmasında ve SIRT1 inhibitörlerinin potansiyel terapötik uygulamalarının araştırılmasında kimyasal bir araç olarak kullanılabilir.

Keywords

• kalsiyum früktoborat
• borik asit
• SIRT1
• enzim aktivitesi

Supporting Institution

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) projeleri tarafından desteklenmiştir.

Project Number

124S255

Ethical Statement

Dokuz Eylül Üniversitesi Girişimsel Olmayan Araştırmalar Etik Kurulu’ndan onay alınmıştır. Karar no: 2021/02-71 Tarih: 18.01.2021

Thanks

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu’na (TÜBİTAK) bu çalışmaya sağladığı mali destek için teşekkür ederiz.

Concentration-Dependent Inhibitory Effect Of Calcium Fructoborate On Sirt1 Enzyme Activity: A Comparative Analysis With Boric Acid

Abstract

Purpose: SIRT1 functions in regulatory mechanisms including cellular aging, apoptosis, glucose and lipid metabolism, oxidative stress and inflammation. Studies show that SIRT1 inhibitors may affect various types of cancer and metabolic disorders, making SIRT1 an important therapeutic target. Boric acid is an inorganic boron compound with antimicrobial, antifungal and potential anticancer effects. Calcium fructoborate, another boron compound found in vegetables and fruits, has anti-inflammatory, antioxidant and anti-tumor effects. This study aimed to investigate the inhibitory potential of calcium fructoborate on SIRT1 activity and to evaluate its possible use as a chemical tool for future studies targeting SIRT1 regulation. Material and Methods: In this study, the effect of calcium fructoborate on SIRT1 activity was investigated by a fluorescence-based method using human recombinant SIRT1. Results: Calcium fructoborate inhibited SIRT1 activity in a dose-dependent manner. Calcium fructoborate significantly inhibited SIRT1 activity by 42% at a concentration of 20 mM (p < 0.001). In the boric acid group, a decreasing trend in SIRT1 enzyme activity was observed, but this difference was not statistically significant. Conclusion: The results provide important basic information. Calcium fructoborate may be used as a chemical tool to elucidate the biological functions of SIRT1 and to investigate the potential therapeutic applications of SIRT1 inhibitors.

Keywords

• calcium fructoborate
• boric acid
• SIRT1
• enzyme activity

Supporting Institution

This study was supported by the Scientific and Technological Research Institution of Turkey Projects (TÜBİTAK).

Project Number

124S255

Ethical Statement

Approval was obtained from the Non-Interventional Research Ethics Committee of Dokuz Eylül University Decision no: 2021/02-71 Date: 18.01.2021

Thanks

We gratefully acknowledge the Scientific and Technological Research Council of Türkiye (TÜBİTAK) for their financial support of this study.

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