B-RAF V600E AND B-RAF CODON 464-469 MUTATIONS IN HAIRY CELL LEUKEMIA PATIENTS AND THEIR RELATION WITH CLINICAL PARAMETERS

Abstract

PURPOSE: Hairy cell leukemia (HCL) is a chronic lymphoproliferative disorder which counts %2-3 percent of the leukemias. B-RAF is a member of mitogen activated protein kinase pathway, associated with cell proliferation. The most common B-RAF mutation V600E has been shown in hairy cell leukemia recently. The aim of our study is to investigate B-RAF V600E and B-RAF codon 464-469 mutations in HCL patients and compare the results with clinical parameters. METHODS: Thirteen individuals who were diagnosed and followed up with hairy cell leukemia in Dokuz Eylul University Faculty of Medicine Hematology outpatient clinic are included in our study. Demographic and clinical data are collected and B-RAF mutations are analyzed with pyrosequencing based molecular methods. RESULTS: B-RAFV600E mutation was positive in 10 (%76,9) patients. B-RAF G464E was mutated in one patient, B-RAF G466E was positive in another and B-RAF G469E mutation has been found in a patient. Two patients had both codon 600 and codon 464-469 mutations, showing no invincible difference clinically. All the patients having lymphadenopathy had B-RAFV600E mutations.(p=1.000) Response rates were similar in the groups having B-RAFV600E mutation and/or B-RAF codon 464-469 mutations. CONCLUSION: B-RAF is a commonly mutated gene in hairy cell leukemia with different types of muatations. Especially B-RAFV600E mutation can be used as a supportive diagnostic test, in cases with contraversial diagnosis or differential diagnosis of other peripheral B cell neoplasms. Also it can be used as a marker to select the candidate patients for target therapies, who did not respond to the conventional therapies.

Keywords

• HAIRY CELL LEUKEMIA
• B-RAF V600E
• B-RAF G464E
• B-RAF G469E

References

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