Objectives: Microglial cells are the central regulators of inflammatory responses in the brain and spinal cord. In addition to surveillance during resting state, they become activated due to microbial molecules and pathological insults. Endogenously expressed Activated protein C (APC) is an anticoagulant molecule with anti-inflammatory and cytoprotective roles, mediated by one of its receptors, Endothelial protein C receptor (EPCR). This study aimed to examine the basal and inducible expression of EPCR and unravel the regulatory mediators of its expression in microglia.
Methods: We studied probable effects of Lipopolysaccharide (LPS), Peptidoglycan (PGN), and Polyinosinic–polycytidylic acid [Poly(I:C)] on EPCR mRNA and protein levels in N9 mouse microglial cells by qPCR and flow cytometry. Then, Cyclosporin A (CsA) and Mithramycin A (MMA) were used to inhibit transcription factors in the promoter region of the EPCR gene, which are Nuclear factor of activated T-cells, cytoplasmic 1 (NFATC1), and specificity protein 1 (Sp1).
Results: As a result, the Sp1 transcription factor’s chemical inhibition impaired the upregulating effects of LPS and PGN on EPCR expression.
Conclusion: Thus, our data suggest that LPS and PGN gave rise to increased EPCR levels in microglia, mainly through the Sp1 transcription factor.
microglia endothelial protein c receptor lipopolysaccharide peptidoglycan Sp1 transcription factor
The Scientific and Technological Research Council of Turkey (TUBITAK)
114Z577
The authors thank Dr. Erden Eren for his technical assistance.
114Z577
Birincil Dil | İngilizce |
---|---|
Konular | Sağlık Kurumları Yönetimi |
Bölüm | Research Article |
Yazarlar | |
Proje Numarası | 114Z577 |
Yayımlanma Tarihi | 26 Şubat 2021 |
Gönderilme Tarihi | 6 Ocak 2021 |
Yayımlandığı Sayı | Yıl 2021 Cilt: 5 Sayı: 1 |