Research Article
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Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue

Year 2022, Volume: 6 Issue: 3, 872 - 876, 29.09.2022
https://doi.org/10.30621/jbachs.1154867

Abstract

Background: Investigation for research on the cellular biology and physiology of colon cancer tissues requires viable dissociation of single cells. The amount of tissue and dissociation methods can affect the amount of single cell viability. Inadequate initial tissue has negative effects on data quality by resulting in insufficient quality and the number of cells.
Methods: In the context of this study different-weigh and different-textured colon tumor tissues have been evaluated to emphasize the importance of initial tissue properties during the operation of tissue processing and cell isolation success. Necrotic areas were also evaluated with the isolated viable cells and the success of 3D primary culture.
Results: Elevated weight of the tissue resulted with more total isolated cells. Necrotic tissues caused low percentage of viable cells. Since resected tissues were bigger than biopsy samples, resected tissues derived primary 3D culture were succesfully maintained the culture.
Conclusion: To conclude, isolated cells from the bigger and non-necrotic tumor tissues showed better growth pattern for 3D cultures. On the other hand, size was found as a crucial parameter for obtaining more viable cancer cells.

Supporting Institution

The study has been supported by Dokuz Eylul University, Scientific Research Projects Coordination Unit

Project Number

2018.KB.SAG.039

Thanks

Thanks to Assoc. Dr. Omer Hidir YILMAZ, Asst.Prof.Dr.Jatin ROPER and Ozge AKCAY for transfering their valuable experience for the study.

References

  • 1. Antoni D, Burckel H, Josset E, Noel G. Three-dimensional cell culture: a breakthrough in vivo. Int J Mol Sci. 2015; 16(3):5517-27.
  • 2. Almeqdadi M, Mana MD, Roper J, Yilmaz ÖH. Gut organoids: mini-tissues in culture to study intestinal physiology and disease. Am J Physiol Cell Physiol. 2019; 317(3):C405-C419.
  • 3. Chaicharoenaudomrung N, Kunhorm P, Noisa P. Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling. World J Stem Cells. 2019; 11(12):1065-1083.
  • 4. Ishiguro T, Ohata H, Sato A, Yamawaki K, Enomoto T, Okamoto K. Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications. Cancer Sci. 2017; 108(3):283-289.
  • 5. Esparza-López J, Martínez-Aguilar JF, Ibarra-Sánchez MJ. Deriving primary cancer cell cultures for personalized therapy. Rev Invest Clin. 2019; 71(6):369-380.
  • 6. Mitra A, Mishra L, Li S. Technologies for deriving primary tumor cells for use in personalized cancer therapy. Trends Biotechnol. 2013 Jun; 31(6):347-54.
  • 7. Li WC, Ralphs KL, Tosh D. Isolation and culture of adult mouse hepatocytes. Methods Mol Biol. 2010; 633:185-96.
  • 8. Harris CC, Leone CA. Some effects of EDTA and tetraphenylboron on the ultrastructure of mitochondria in mouse liver cells. J Cell Biol. 1966 Feb; 28(2):405-8.
  • 9. Tuveson D, Clevers H. Cancer modeling meets human organoid technology. Science. 2019; 364(6444):952-955.
  • 10. Cabrera MC, Hollingsworth RE, Hurt EM. Cancer stem cell plasticity and tumor hierarchy. World J Stem Cells. 2015; 7(1):27-36.
Year 2022, Volume: 6 Issue: 3, 872 - 876, 29.09.2022
https://doi.org/10.30621/jbachs.1154867

Abstract

Project Number

2018.KB.SAG.039

References

  • 1. Antoni D, Burckel H, Josset E, Noel G. Three-dimensional cell culture: a breakthrough in vivo. Int J Mol Sci. 2015; 16(3):5517-27.
  • 2. Almeqdadi M, Mana MD, Roper J, Yilmaz ÖH. Gut organoids: mini-tissues in culture to study intestinal physiology and disease. Am J Physiol Cell Physiol. 2019; 317(3):C405-C419.
  • 3. Chaicharoenaudomrung N, Kunhorm P, Noisa P. Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling. World J Stem Cells. 2019; 11(12):1065-1083.
  • 4. Ishiguro T, Ohata H, Sato A, Yamawaki K, Enomoto T, Okamoto K. Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications. Cancer Sci. 2017; 108(3):283-289.
  • 5. Esparza-López J, Martínez-Aguilar JF, Ibarra-Sánchez MJ. Deriving primary cancer cell cultures for personalized therapy. Rev Invest Clin. 2019; 71(6):369-380.
  • 6. Mitra A, Mishra L, Li S. Technologies for deriving primary tumor cells for use in personalized cancer therapy. Trends Biotechnol. 2013 Jun; 31(6):347-54.
  • 7. Li WC, Ralphs KL, Tosh D. Isolation and culture of adult mouse hepatocytes. Methods Mol Biol. 2010; 633:185-96.
  • 8. Harris CC, Leone CA. Some effects of EDTA and tetraphenylboron on the ultrastructure of mitochondria in mouse liver cells. J Cell Biol. 1966 Feb; 28(2):405-8.
  • 9. Tuveson D, Clevers H. Cancer modeling meets human organoid technology. Science. 2019; 364(6444):952-955.
  • 10. Cabrera MC, Hollingsworth RE, Hurt EM. Cancer stem cell plasticity and tumor hierarchy. World J Stem Cells. 2015; 7(1):27-36.
There are 10 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Ender Berat Ellidokuz 0000-0001-5863-3298

Tolga Sever 0000-0002-0135-0243

Gizem Çalıbaşı Koçal 0000-0002-3201-4752

Aras Emre Canda 0000-0002-8257-5881

Sulen Sarioglu 0000-0003-4877-3064

Hasan Kurter 0000-0001-6851-5279

İlhan Öztop 0000-0002-0425-0651

Yeşim Tunçok 0000-0002-4049-336X

Yasemin Başbınar 0000-0001-9439-2217

Project Number 2018.KB.SAG.039
Publication Date September 29, 2022
Submission Date August 4, 2022
Published in Issue Year 2022 Volume: 6 Issue: 3

Cite

APA Ellidokuz, E. B., Sever, T., Çalıbaşı Koçal, G., Canda, A. E., et al. (2022). Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue. Journal of Basic and Clinical Health Sciences, 6(3), 872-876. https://doi.org/10.30621/jbachs.1154867
AMA Ellidokuz EB, Sever T, Çalıbaşı Koçal G, Canda AE, Sarioglu S, Kurter H, Öztop İ, Tunçok Y, Başbınar Y. Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue. JBACHS. September 2022;6(3):872-876. doi:10.30621/jbachs.1154867
Chicago Ellidokuz, Ender Berat, Tolga Sever, Gizem Çalıbaşı Koçal, Aras Emre Canda, Sulen Sarioglu, Hasan Kurter, İlhan Öztop, Yeşim Tunçok, and Yasemin Başbınar. “Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue”. Journal of Basic and Clinical Health Sciences 6, no. 3 (September 2022): 872-76. https://doi.org/10.30621/jbachs.1154867.
EndNote Ellidokuz EB, Sever T, Çalıbaşı Koçal G, Canda AE, Sarioglu S, Kurter H, Öztop İ, Tunçok Y, Başbınar Y (September 1, 2022) Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue. Journal of Basic and Clinical Health Sciences 6 3 872–876.
IEEE E. B. Ellidokuz, T. Sever, G. Çalıbaşı Koçal, A. E. Canda, S. Sarioglu, H. Kurter, İ. Öztop, Y. Tunçok, and Y. Başbınar, “Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue”, JBACHS, vol. 6, no. 3, pp. 872–876, 2022, doi: 10.30621/jbachs.1154867.
ISNAD Ellidokuz, Ender Berat et al. “Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue”. Journal of Basic and Clinical Health Sciences 6/3 (September 2022), 872-876. https://doi.org/10.30621/jbachs.1154867.
JAMA Ellidokuz EB, Sever T, Çalıbaşı Koçal G, Canda AE, Sarioglu S, Kurter H, Öztop İ, Tunçok Y, Başbınar Y. Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue. JBACHS. 2022;6:872–876.
MLA Ellidokuz, Ender Berat et al. “Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue”. Journal of Basic and Clinical Health Sciences, vol. 6, no. 3, 2022, pp. 872-6, doi:10.30621/jbachs.1154867.
Vancouver Ellidokuz EB, Sever T, Çalıbaşı Koçal G, Canda AE, Sarioglu S, Kurter H, Öztop İ, Tunçok Y, Başbınar Y. Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue. JBACHS. 2022;6(3):872-6.