Tissue Processing and Isolation of Tumor Cells from Human Colorectal Cancer Tissue

Yıl 2022, Cilt: 6 Sayı: 3, 872 - 876, 29.09.2022

Ender Berat Ellidokuz , Tolga Sever , Gizem Çalıbaşı Koçal , Aras Emre Canda , Sulen Sarioglu , Hasan Kurter , İlhan Öztop , Yeşim Tunçok , Yasemin Başbınar

https://doi.org/10.30621/jbachs.1154867

Öz

Background: Investigation for research on the cellular biology and physiology of colon cancer tissues requires viable dissociation of single cells. The amount of tissue and dissociation methods can affect the amount of single cell viability. Inadequate initial tissue has negative effects on data quality by resulting in insufficient quality and the number of cells.
Methods: In the context of this study different-weigh and different-textured colon tumor tissues have been evaluated to emphasize the importance of initial tissue properties during the operation of tissue processing and cell isolation success. Necrotic areas were also evaluated with the isolated viable cells and the success of 3D primary culture.
Results: Elevated weight of the tissue resulted with more total isolated cells. Necrotic tissues caused low percentage of viable cells. Since resected tissues were bigger than biopsy samples, resected tissues derived primary 3D culture were succesfully maintained the culture.
Conclusion: To conclude, isolated cells from the bigger and non-necrotic tumor tissues showed better growth pattern for 3D cultures. On the other hand, size was found as a crucial parameter for obtaining more viable cancer cells.

Anahtar Kelimeler

Colorectal cancer, chemical dissociation, cancer cell isolation

Destekleyen Kurum

The study has been supported by Dokuz Eylul University, Scientific Research Projects Coordination Unit

Proje Numarası

2018.KB.SAG.039

Teşekkür

Thanks to Assoc. Dr. Omer Hidir YILMAZ, Asst.Prof.Dr.Jatin ROPER and Ozge AKCAY for transfering their valuable experience for the study.

Kaynakça

• 1. Antoni D, Burckel H, Josset E, Noel G. Three-dimensional cell culture: a breakthrough in vivo. Int J Mol Sci. 2015; 16(3):5517-27.
• 2. Almeqdadi M, Mana MD, Roper J, Yilmaz ÖH. Gut organoids: mini-tissues in culture to study intestinal physiology and disease. Am J Physiol Cell Physiol. 2019; 317(3):C405-C419.
• 3. Chaicharoenaudomrung N, Kunhorm P, Noisa P. Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling. World J Stem Cells. 2019; 11(12):1065-1083.
• 4. Ishiguro T, Ohata H, Sato A, Yamawaki K, Enomoto T, Okamoto K. Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications. Cancer Sci. 2017; 108(3):283-289.
• 5. Esparza-López J, Martínez-Aguilar JF, Ibarra-Sánchez MJ. Deriving primary cancer cell cultures for personalized therapy. Rev Invest Clin. 2019; 71(6):369-380.
• 6. Mitra A, Mishra L, Li S. Technologies for deriving primary tumor cells for use in personalized cancer therapy. Trends Biotechnol. 2013 Jun; 31(6):347-54.
• 7. Li WC, Ralphs KL, Tosh D. Isolation and culture of adult mouse hepatocytes. Methods Mol Biol. 2010; 633:185-96.
• 8. Harris CC, Leone CA. Some effects of EDTA and tetraphenylboron on the ultrastructure of mitochondria in mouse liver cells. J Cell Biol. 1966 Feb; 28(2):405-8.
• 9. Tuveson D, Clevers H. Cancer modeling meets human organoid technology. Science. 2019; 364(6444):952-955.
• 10. Cabrera MC, Hollingsworth RE, Hurt EM. Cancer stem cell plasticity and tumor hierarchy. World J Stem Cells. 2015; 7(1):27-36.