In vitro Antagonistic Activity against Fusarium Species of Local Trichoderma spp. Isolates

Year 2017, Volume: 11 Issue: 32, 67 - 74, 06.09.2017

Çiğdem Küçük

Abstract

Trichoderma spp. isolates were isolated from rhizosphere soils of cotton and maize. The antagonistic effects of local isolates of Trichoderma sp. against some Fusarium species such as Fusarium solani, F.moniliforme, F.culmorum, F.verticillioides and F.chlamydosporum were studied by using the dual culture technique. The isolates of Trichoderma spp. had a inhibitory effects on the growth of tested Fusarium species. Also, the influence of abiotic stress such as temperature, drought and NaCl on the growth of Trichoderma isolates was studied in this study. The most resistant isolates to abiotic stress were T8, T12 and T14, respectively. The specific activity of chitinase produced by local isolates was tested. The antagonist T8 isolate produced higher chitinase activity in culture supernatant.

Keywords

Abiotic stress , Chitinase activity , Local isolate , Trichoderma spp.

References

• Akladlous SA and Abbas SM. (2014). Application of Trichoderma harzianum T22 as a biofertilizer potential in maize growth. Journal of Plant Nutrition, 37: 30-49.
• Amalraj LD, Kumar P, Desai S and Akmed MH (2010). In vitro Characterization of Trichoderma viride for abiotic stress tolerance and field evaluation against root rot disease in Vigna mungo L. Journal of Biofertilizer and Biopesticid, 2: 2-5.
• Arjona-Girona I, Vinale F, Ruano-Rosa D, Lorito M and Lopez-Herrera CJ (2014). Effect of metabolites from different Trichoderma strains on the growth of Rosellinia necatrix, the causal agent of avacado white root rot. European Journal of Plant Pathology, 140: 385-397.
• Altınok HH and Erdoğan O (2015). Determination of the in vitro effect of Trichoderma harzianum on phytopathogenic strains of Fusarium oxysporum. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43: 494-500.
• Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72: 248-254.
• Dennis C and Webster J (1971). Antagonism properties of species groups of Trichoderma, III hypal intreactions. Transactions of the British Mycological Society, 57: 363-369.
• Elad Y and Chet I, Henis Y (1981). A selective medium for improving quantitative isolation of Trichoderma spp. from soil. Phytoparasitica, 9: 59-67.
• Elad Y, Chet I, and Henis Y (1982). Degradation of plant pathogenic fungi by Trichoderma harzianum. Canadian Journal of Microbiology, 28: 719-725.
• El-Katatny MH, Abdelzaher MA and Shoulkamy MA (2006). Antagonistic actions of Pythium oligandrum and Trichoderma harzianum against phytopathogenic fungi (Fusarium oxysporum and Pythium uitimum var. ultimum). Archives of Phytopathology and Plant Protection, 39: 289-301.
• Ferrigo D, Raiola A, Rasera R and Causin R. (2014). Trichoderma harzianum seed treatment controls Fusarium verticillioides colonization and fumonisin contamination in maize under field conditions. Crop Protection, 65: 51-56.
• Gams W, Anderson TH and Domsch W. (1980). Compendium of soil fungi. Academic Press (London) Ltd., London, UK. p 860.
• Ghanbarzadeh B, Safaie N, Goltapeh EM (2010). Antagonistic activity and hyphal interactions of Trichoderma spp. against Fusarium proliferatum and F.oxysporum in vitro. Archives of Phytopathology and Plant Protection, 47: 1979-1987.
• Harman GE, Hayes CK, Lorito M, Broadway RM, Di Petro A, Peterbauer CK and Transmo A (1993). Chitinolytic enzymes of Trichoderma harzianum: Purification of chitobiosidase and endochitinase. Phytopathology, 83:313-318.
• Harman GE, Howell CR, Viterbo A, Chet I and Lorito M (2004). Trichoderma species-opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2: 43-56.
• Howell CR (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease, 87: 4-10.
• Kim TG and Knudsen G (2013). Relationship between the biocontrol fungus Trichoderma harzianum and the phytopathogenic fungus Fusarium solani f.sp. pisi. Applied Soil Ecology, 68: 57-60.
• Küçük Ç and Kıvanç M (2004). In vitro Antifungal Activity of Strains of Trichoderma harzianum. Turkish Journal of Biology, 28: 111-115.
• Kredics L, Antal Z, Manczinger L, Szekercs A, Kevei F and Nagy E (2003). Influence of environmental parameters on Trichoderma strains with biocontrol potential. Food Technology and Biotechnology, 41:37-42.
• Li YT, Hwang SG, Huang Y and Huang CH (2017). Effects of Trichoderma asperellum on nutrient uptake and Fusarium wilt of tomato. Crop Protection, 2:1-8.
• Mairzano M, Gallo A and Altomare C (2013). Improvement of biocontrol efficacy of Trichoderma harzianum vs. Fusarium oxysporum f.sp. lycopersici through UV-induced tolerance to fusaric acid. Biological Control, 67:397-408.
• Mohamed HAA and Haggag WM (2005). Biocontrol Potential of Salinity Tolerant Mutants of Trichoderma harzianum against Fusarium oxysporum Causing Tomato Wilt Disease. Arab Journal of Biotechnology, 8: 35-48.
• Mazhabi M., Nemati H., Rouhani H., Tehranifar A., Mahdikhani-moghadam E. and Kaveh H. (2011). How May Trichoderma Application Affect Vegetative and Qualitative Traits in Tulip "Darwin Hybride" Cultivar. Journal of Biological & Environmental Sciences, 5:177-182.
• Nakkeeran S, Renukadevi P and Marimuthu T (2005). Antagonistic potentiality of Trichoderma viride and assessment of its efficacy for the management of cotton root rot. Archives of Phytopathology and Plant Protection, 38:209-225.
• Rawat L, Singh Y, Shukla N and Kumar J (2013). Salinity tolerant Trichoderma harzianum reinforces NaCl tolerance and reduces population dynamics of Fusarium oxysporum f.sp. ciceri in chickpea (Cicer arietinum L.) under salt stress conditions. Archives of Phytopathology and Plant Protection, 46:1442-1467.
• Rifaii MA (1969). A. Revision of the genus Trichoderma. Mycological Papers, 116: 1-56.
• Saravanakumar K, Li YQ, Yu CJ, Wang QQ, Wang M, Sun JA, Gao JX and Chen J (2017). Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium talk rot. Scientific Reports, 7: 1-13.
• Shores M, Harman GE and Mastouri F (2010). Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology, 48: 21-23.
• Şehirli S. and Saydam C. (2016). The Effect of Acetic, Formic and Propionic Acids on Plant Pathogenic Fungi. Journal of Biological & Environmental Sciences, 30:129-137.
• Poosapati S, Ravulapalli PD, Tippirishetty N, Vishwanathaswamy PM and Chunduri S (2014). Selection of high temperature and salinity tolerant Trichoderma isolates with antagonistic activity against Sclerotium rolfsii. Springerplus, 3: 1-11.
• Singh BN, Singh A, Singh BR and Singh HB (2013). Trichoderma harzianum elicits induced resistance in sunflower challanged by Rhizoctonia solani. Journal of Applied Microbiology, 116: 654-666.
• Srivastava RK, Sing RK and Kumar N, Sing S (2010). Management of the Macrophomina disease complex in jute (Corchus olitorius) by Trichoderma viride. Journal of Biological Control, 24:77-79.
• Srivastava M, Pandey S, Shamid M, Sharma A, Singh A and Kumar V (2014). Induction of chitinase, β-glucanase, and xylanase taken from Trichoderma sp. on diffrenet sources: A review. African journal of Microbiology Research, 8: 3131-3136.
• Vgas A, Bhardwaj P, Kumar M, Pachouri UC, Garg S and Singh J (2014). Biochemical characterization of plant pathogenic fungal cultures and their control Trichoderma harzianum. National Academy Science Letters, 37: 435-439.
• Yu Y, Yang Z, Guo K, Li Z, Zhou H, Wei Y, Li J, Zhang X, Harvey P and Yang H. (2015). Oxidative damage induced by heat stress could be relieved by nitric oxide in Trichoderma harzianum LTR-2. Current Microbiology, 70: 618-622.
• Yurtsever N (1984). Deneysel istatistik metodlari. Tarim Orman Köy Hizmetleri Gen. Müd. Toprak ve Gübre Arastirma Müd. Yayınları. No. 121, s.623, Ankara