Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper

Cansu Saydam; Ümit Arslan; İbrahim Özer Elibüyük

Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper

Abstract

In this work, the effects of the plant activators Messenger, Actigard, ISR-2000 and Crop-Set against Potato Virus Y (PVY) in pepper (Capsicum annuum) were evaluated. In surveys conducted in Adana and Mersin provinces of southern Turkey in 2015, PVY was detected in symptomatic peppers by DAS-ELISA tests. The PVY isolate used in this study was isolated from pepper plants in Adana. Effects of the activators were evaluated in a climate chamber. Pepper seedlings were treated by Messenger, Actigard, ISR-2000 and Crop-Set and then the activator-treated peppers were inoculated by the PVY isolate 72 and 96 hrs after the activator treatments. In the assay, symptom expression time, plant length, leaf width, root length, lignification (lignin accumulation) and H2O2 accumulation were measured until the flowering period. The data was analyzed by using statistical methods. Messenger, Actigard, ISR-2000 and Crop-Set delayed symptom expression time by 12, 10, 8 and 3 days, respectively. Moreover Actigard, Messenger and ISR-2000 increased leaf surface area and plant height. Among all treatments, only inoculations with PVY 72 hrs after Crop-Set treatments increased the root length. Activator treated plants showed lignification and H2O2 accumulation. Best treatment times for lignification and H2O2 accumulation were determined as 72 hrs for Messenger, 96 hrs for Actigard, Crop-Set and ISR-2000.

Keywords

Pepper, PVY, Actigard, Messenger, ISR-2000, Crop-Set, Activator, Lignification, Hydrogen peroxide accumulation

References

Anfoka GH (2000). Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato (Lycopersicon esculentum. Mill cv. Vollendung) to Cucumber mosaic virus. Crop Prot., 19: 40-405. Anonymous (2015). URL:http://www.aresorganik.com.tr/urungor.asp?id=848&urun=Isr% 20-% 202000 (Erişim tarihi: 26.05.2015).
Anonymous (2017). URL: http://www.syngentacropprotection.com/pdf/labels/scp922al2l0108.pdf (Erişim tarihi: 30.03.2017). Arli-Sokmen M, and Sevik MA (2005).Viruses infecting field-grown tomatoes in Samsun province, Turkey. Arch Phytopath Plant Protect., 39 (4): 283-288.
Balázs B, József F, Miklós P, Zoltán K (2003). Role of reactive oxygen species and antioxidants in plant disease resistance. Pest Manag. Sci., 59: 375–503.
Büchen-Osmond C (1987). Plant viruses online-descriptions and lists from the VIDE database potato Y potyvirus. http://image.fs.uidaho.edu/vide/descr652.html. (Erişim tarihi: 22.06.2015).
Buzkan N, Arpaci BB, Simon V, Fakhfakh H, and Moury B (2013). High prevalence of poleroviruses in field-grown pepper in Turkey and Tunisia. Arch Virol. 158 (4): 881-815.
Çalışkan AF, and Kamberoğlu MA (2010). Kabak sarı mozayik virüsü (Zucchini yellow mosaic virus, ZYMV)’nün tanısı ve bitki aktivatörleri kullanılarak mücadele olanaklarının araştırılması. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22: 58-65.
Çelik İ, Özalp R, Çelik N, Polat İ, Sülü G, and Ünlü A (2013). Patates Y virüsü (Potato Virus Y = PVY)’ne dayanıklı sivri biber hatlarının geliştirilmesi. Derim, 30: 42-53.
Clark MF, and Adams AN (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34: 75-83.
Collendavelloo J, Legrand M, and Fritig B (1983). Plant Disease and the Regulation of Enzymes Involved in Lignification. Plant Physiol., 73: 550-554.
Csinos AS, and Pappu HR (2001). Management of tomato spotted wilt virus in flue-cured tobacco with Acibenzolar-S-Methyl and Imidacloprid. Plant Dis., 85: 292-296.

De Bokx J A, and Huttinga H (1981). Potato virus Y. URL: http://www.dpvweb.net/dpv/ showdpv.php?dpvno=242 (Erişim tarihi: 23.06.2015).
Dereboylu AE and Tort N (2010). Bazı aktivatör ve fungisit uygulamalarının Cucumis sativus L. (Hıyar) bitkisinde verim-kalite üzerine etkisi. Çukurova Üniversitesi Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi, 31: 30-42.
Flaudung M, and Ritter E (1991). Plant leaf area measurements by personal computers. J. Agron. Crop Sci., 166: 69-70.
Gahan PB (1984). Plant histochemistry and cytochemistry: an introduction. Orlando: Academic Press.
Godard FJ, Ziadi S, Monot C, Corre LD and Silué D (1999). Benzothiadiazole (BTH) induces resistance in cauliflower (Brassica oleracea var. botrytis) to downy mildew of crucifers caused by Peronospora parasitica. Crop Prot., 18: 397-405.
Hafez YM, Bacsó R, Király Z, Künstler A, and Király L (2012). Up-Regulation of antioxidants in tobacco by low concentrations of H2O2 suppresses necrotic disease symptoms. Biochem. Cell Biol., 102:848-856.
Kashtban AH, Elibüyük İÖ (2010). Bal kabağında kabak sarı mozaik virus’una karşı bazı bitki aktivatörlerinin etkilerinin belirlenmesi. Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Ankara.
Kiracı S, and Padem H (2015). Havuç yetiştiriciliğinde bitki aktivatörü ve mikrobiyal gübre uygulamalarının verim ve bazı fizikokimyasal parametreler üzerine etkisi. SDÜ., Ziraat Fakültesi Dergisi, 10: 65-72.
Koca YO (2003). İki bitki aktivatörünün patateste bazı tarımsal özellikler üzerine etkileri. Yüksek Lisans Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, Tarla Bitkileri Anabilim Dalı, İzmir.
Li H, Dingi X, Wangi C, Kei H, Wu Z, Wang Y, Liu H, and Guo J (2016). Control of Tomato yellow leaf curl virus disease by Enterobacter asburiae BQ9 as a result of priming plant resistance in tomatoes. Turk. J. Biol., 40: 150-159.
Liao WB, Zhang ML, and Yu Ji-Hua. (2013) Role of nitric oxide in delaying senescence of cut rose flowers and its interaction with ethylene. Sci. Hort-Amsterdam 155: 30–38.
Madhusudhan KN, Deepak SA, Prakash HS, Agrawal GK, Jwa NS, and Rakwal R (2008). Acibenzolar-s-methyl (ASM)-induced resistance against tobamoviruses involves induction of RNA dependent RNA polymerase (RdRp) and alternative oxidase (AOX) genes. J. Crop Sci. Biotechnol., 11: 127-134.
Mandal B, Mandal S, Csinos A S, Martinez N, Culbreath AK, and Pappu HR (2008). Biological and molecular analyses of the acibenzolar-s-methyl- induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus. Phytopathology, 98: 196-204.
Mejía DMA, Rodas EIG, Patiño LFH, and González EPJ (2009). Effect of acibenzolar-s-methyl on virus infection progress caused by potyvirus in tree tomato. Agron. Colomb., 27: 87-93.
Momol MT, Olson SM, Funderburk JE, Stavisky J, and Marois JJ (2004). Integrated management of tomato spotted wilt on field grown tomatoes. Plant Dis., 88: 882-890.
Nair C B, Anith KN, and Sreekumar J (2007). Mitigation of growth retardation of the plant defense activator, acibenzolar-s-methyl, in amaranthus plants by plant growth-promoting rhizobacteria. World J. Microbiol. Biotechnol., 23: 1183-1187.
Nyamwamu PA, Mukoye B, Osogo AK, Omuse CN, Ajanga S, and Were HK (2014). Distribution and biological characterization of potato virus Y in Kenya. J. Agri-Food Appl. Sci., 2: 258-264.
Ozaslan M, Aytekin T, Bas B, Kilic IH, Afacan ID, and Dag DS (2006). Virus diseases of cucurbits in Gaziantep-Turkey. Plant Pathol J., 5 (1): 24-27.
Roberts T, and Hutson D (1999). Metabolic Pathways of Agrochemicals, Part two: Insecticides and Fungicides. Cambridge, UK: Royal Society of Chemistry, 1447 p.
Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner H, and Hunt MD (1996). Systemic Acquired Resistance. The Plant Cell, 8: 1809-1819.
Saravanakumar D, Charles VN, and Kumarb R (2007). PGPR-induced defense responses in the tea plant against blister blight disease. Crop Prot., 26: 556-565. Schonbeck F, Steiner U, and Kraska T (1993). Induced resistance: criteria, mechanisms, practical application and estimation. J. Plant Dis. Prot., 100: 541-557.
Sevik MA (2011). Occurrence of pepper mild mottle virus (PMMoV) in greenhouse-grown pepper (Capsicum annuum L.) in the west Mediterranean region of Turkey. Afr. J. Biotechnol., 10 (25): 4976-4979.
Soylu EM (1999). Mildiyö etmeni (Peronospora parasitica) ve konukçusu Arabidopsis thaliana arasındaki ilişkilerin sito ve histokimyasal yöntemlerle incelenmesi. Doktora Tezi. Akdeniz Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, 160 s, Antalya.
Taraku N, and Juretic N (1991). Potato virus Y on tobacco in Kosovo. Acta Bot. Croat. 50: 19-23. Tosun N, and Ergün A (2002). Bitkisel Üretimde ve Tarımsal Savaşımda Yeni Bir Yaklaşım Olarak Bitki Aktivatörlerinin Rolü. Tarım ve Köyişleri Bakanlığı Ege Tarımsal Araştırma Enstitüsü Müdürlüğü Yayın No: 109, Tarımsal Araştırma Yayım ve Koordinasyonu, 251-263.
Tsuda M (1999). Errors in leaf area measurement with an automatic area meter due to leaf chlorophyll in crop plants. Ann. Bot., 84: 799-801.
Vallet C, Chamber B, Czaninski Y, and Monties B (1996). Histochemistry of lignin deposition during sclerenchyma differentiation in Alfalfa stems. Annals Bot., 78: 625-632.
Van Loon LC, Bakker PA, and Pieterse CM (1998). Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol., 36: 453-483.
Zhou X, Zhu X, Sun G, Li W, Zhang Y, Wu H, and Gao X (2015). Overproduction of high purity HapG (Xooc) from Xanthomonas oryzae functions as a growth and antivirus factor in tobacco. Asia Life Sci., 24: 1-9.

Details

Primary Language

English

Subjects

Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Cansu Saydam
Türkiye

Ümit Arslan ^*
Türkiye

İbrahim Özer Elibüyük
Türkiye

Publication Date

September 6, 2017

Submission Date

June 20, 2017

Acceptance Date

August 11, 2017

Published in Issue

Year 2017 Volume: 11 Number: 32

IZ

https://izlik.org/JA53NB24TJ

APA

Saydam, C., Arslan, Ü., & Elibüyük, İ. Ö. (2017). Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper. Journal of Biological and Environmental Sciences, 11(32), 93-103. https://izlik.org/JA53NB24TJ

AMA

1.Saydam C, Arslan Ü, Elibüyük İÖ. Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper. JBES. 2017;11(32):93-103. https://izlik.org/JA53NB24TJ

Chicago

Saydam, Cansu, Ümit Arslan, and İbrahim Özer Elibüyük. 2017. “Determination of Effects of Some Plant Activators Against Potato Virus Y in Pepper”. Journal of Biological and Environmental Sciences 11 (32): 93-103. https://izlik.org/JA53NB24TJ.

EndNote

Saydam C, Arslan Ü, Elibüyük İÖ (September 1, 2017) Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper. Journal of Biological and Environmental Sciences 11 32 93–103.

IEEE

[1]C. Saydam, Ü. Arslan, and İ. Ö. Elibüyük, “Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper”, JBES, vol. 11, no. 32, pp. 93–103, Sept. 2017, [Online]. Available: https://izlik.org/JA53NB24TJ

ISNAD

Saydam, Cansu - Arslan, Ümit - Elibüyük, İbrahim Özer. “Determination of Effects of Some Plant Activators Against Potato Virus Y in Pepper”. Journal of Biological and Environmental Sciences 11/32 (September 1, 2017): 93-103. https://izlik.org/JA53NB24TJ.

JAMA

1.Saydam C, Arslan Ü, Elibüyük İÖ. Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper. JBES. 2017;11:93–103.

MLA

Saydam, Cansu, et al. “Determination of Effects of Some Plant Activators Against Potato Virus Y in Pepper”. Journal of Biological and Environmental Sciences, vol. 11, no. 32, Sept. 2017, pp. 93-103, https://izlik.org/JA53NB24TJ.

Vancouver

1.Cansu Saydam, Ümit Arslan, İbrahim Özer Elibüyük. Determination of Effects of Some Plant Activators against Potato Virus Y in Pepper. JBES [Internet]. 2017 Sep. 1;11(32):93-103. Available from: https://izlik.org/JA53NB24TJ

Journal of Biological and Environmental Sciences is the official journal of Bursa Uludag University

Bursa Uludag University, Gorukle Campus, 16059, Bursa, Türkiye.