Evaluation of Nutritional Effects of Different Levels of Enzymatic Fructose and Sugar Syrup on the Half Life and Bacterial Flora of the Bee Digestive System

Year 2020, Volume: 14 Issue: 40 , 45 - 53 , 26.06.2020

Majid Sadreriahi Dehkordi Hamid Reza Karimi Saeed Abadi , Amir Davar Foroozandeh Shahraki Mohammad Behjatian Esfahani Masoud Boroumand Jazi Abdulahad Shaddel Teli Habib Aghdam Shahryar

https://izlik.org/JA28BD69GZ

Abstract

The research was carried out in a completely randomized design with 6 treatments and 6 replications in the bee incubator (Apis Mellifera Meda) from the second half of May 2019 till the end of July 2018 in Isfahan Agricultural and Natural Resources Research Center. In order to evaluate the nutritional effects of different levels of enzymatic fructose (0, 10, 20, 30, 40 and 50%) and sugar (50, 40, 30, 20, 10 and 0%) on the longevity and bacterial flora of the bee digestive system, mean comparison between experimental treatments showed that the lowest longevity and half-life between experimental levels was related to treatment with 50% fructose (P <0.05). The longevity of control treatment and treatments with 10% fructose were significant at 38.8 and 32.2 days, respectively, and the lowest feed intake was also observed at 50% fructose (P <0.05). Maximum and minimum Lactobacillus bacteria population in the bee digestive system inside cage were 10% fructose with 8.30(log CFU/g) and 50% fructose, respectively. The bacterial population of coliforms in the digestive system of bees inside the cage was the lowest with 10% fructose and the highest with 50% fructose (P <0.05).

Keywords

Incubator , Longevity , Lacto bacillus , Coliform , Digestive system , Treatments

References

• Abadi et al 2018. Impact of various carbohydrate sources on functional attributes, colony population, feed intake, and quality of honey produced by the honeybee. Journal of Kafkas Univ Vet Fak Derg, 24 (4): 503-510.
• Alaux et al 2010. Diet Effects On Honeybee Immune competence. Journal of Biology Letters, 6(4): 562-566.
• Barker R.J. and Lehner Y 1978. Laboratory comparison of high fructose corn syrup, grape syrup, honey, and sucrose syrup as maintenance food for caged honey bees.
• Bignell DE, Heath LA 1985. Electropositive redox state of the fifth-instar larval gut of Apis mellifera. Journal of Apiculture Research 24 (4): 211- 252.
• Chapman RF. 1982. The Insects: Structure and Function. Harvard University Press.
• El-Leithy M.A. and El-Sibaei K.B 1972. External and internal microflora of the honey bee (A. mellifera L.). Egyptian Journal of Microbiology.7: 79-87.
• El-leithy M.A. and El-sibael K.B. 1992. Role of microorganisms isolated from bees, its ripening and fermentation of honey. Egyptian Journal of Microbiology. 75:679–681
• Gary N. E. and Lorenzen K 1988. Substage Illumination device for restraining flight of honey bees (Hymenoptera: Apidae) and positively. Photo tactic Insects, 7: 79-87
• Gilliam M., Roubik D.W. and Lorenz BJ 1990. Microorganisms associated with pollen, honey, and brood provisions in the nest of a stingless bee. Melipona fasciata. Apidologie. 21: 89-97.
• Grabow WOK, Hilner CA, Coubrough P 1981. Evaluation of Standard and Modified M-FC. , MacConkey. , and Teepol Media for Membrane Filtration Counting of Fecal Coliforms in Water. Applied and Environmental Microbiology 42 (2):192-199.
• Hendriksma H.P. and Shafir S 2016. Honey Bee Foragers Balance Colony Nutritional Deficiencies. Journal of Behavioral Ecology and Sociobiology 70(4): 509–517
• Hrassnigg N. and Crailsheim K 2005. Differences in drone and worker Physiology in honeybees (A. mellifera). Journal of Apidology. 36(2): 255–277.
• Jachimowicz T, El Sherbiny G 1975. Zur problematik der verwendung von invertzucker fur die bienenfutterung [Problems of invert sugar as food for honey bees]. Apidologie 6: 121-143.
• Johansson TSK .and Johansson MP 1976. Feeding sugar to bees. Bee World 57: 137-143; 58(1): 11-18; 58(2): 49-52.
• Karimi Saeed abadi et al 2019. The effect of various carbohydrate sources on half-life, biological traits and microbial flora of the bee digestive system. Journal of Biological and Environmental Sciences.13(37): 9-18 Keeping.PP.153-171
• khojasteh Shalmany S. and Shivazad M. 2006. The effect of diet propolis supplementation on ross broilers performance. International Journal of Poultry Science, 5:84
• Kunieda T et al 2006. Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome. Insect Molecular Biology 15(5): 563-576.
• Menezes C. et al 2013. The role of useful microorganisms’ stingless bees and stingless bee.
• Piccini C., Antunez K and Zunino P. 2004. An approach to the characterization of the honey bee hive bacterial flora. Apicultural Reserch: 43(3): 101-104.
• Rekiel A et al. 2007. Effect of addition of feed antibiotic flavomycin or prebiotic BIOMOS on production results of fatteners, blood biochemical parameters, morphometric indices of intestine and composition of microflora. Archives of Animal Breeding. Tierzucht. Dummerstorf; 50 (2): 172-80.
• Rinderer TE, Baxter JR 1980. Honeybee hoarding of high fructose corn syrup and cane sugar syrup. American Bee Journal 120: 817-818.
• Rosa CA et al 2003. Yest communities associated with stingless bees.FEMS Yeast Reasearch.4:271-275
• Ruiz-Matute et al 2010. Carbohydrate composition of High-Fructose Corn Syrups (HFCS) used for bee feeding: effect on honey composition. Journal of Agriculture and Food Chemistry 58: 7317-7322
• Sammataro D,Weiss M 2000. Comparison of productivity of colonies of honey bees, Apis mellifera, supplemented with sucrose or high fructose corn syrup. Journal of Insect.
• Sarbojoy S 2018. Isolation, Detection and Characterization of Aerobic Bacteria from Honey Samples of Bangladesh. Journal of biorxiv.
• SAS Institue. 2000. Institue SAS SQL Procedure User Guide, Version 9. SAS, Cary, ISBN: 13:978-158025599X, 576.
• SAS Institute. 2008. SAS User's guid Version 9. 2 Review Edition, Cary NC; SAS Institute Inc, 211 P.
• Stangaciu S 1977. A Guide to propolise origin, uses in the hive characteristics, Composition, properties, indications, counter -Indication. Human Uses, Administration and Products. Available from://www. Apiterapiapia.org/dr-stefan- stangaciu. Htm [Accessed 6 september
• Tajabadi N et al 2011. Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. Apidologie. 42:642–64910
• Tajabadi N et al 2014. Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee. 15;44(3):717-22
• Van den Bogaard AE. 1997. Antimicrobial resistance- relation to human and animal exposure to antibiotics. Journal ofAntimicrobial Chemotherapy. 40 (1): 453-454.
• Van Engelsdorp D et al 2010. An estimate of managed colony losses in the winter: A Report Commissioned by the Apiary
• Weiss M 2009. Supplemental Carbohydrates in Apiculture: Effects upon Honey Bee (Apis mellifera) Health and Productivity. M. S. thesis, Department of Entomology, University of Arizona, Tucson. Science, Vol. 13:1-13.
• Yoshihama M.and Kimura K 2009. Bacteria in the gur of jpanese honey bee Apis cerana Japonica, and their antagonistic effect against paenibacillus larvae, the causal agent of American foulbrood journal of Invertebrate pathology.102:91-96