Determination of Some Disease Resistance Genes of Tomato Breeding Lines Which Developed from Different Origine Using Molecular Markers

Year 2023, Volume: 17 Issue: 49, 1 - 6, 01.03.2024

Ayşe Şeker , Tuğba Zeynep Çardak Merve Yiğit , Emre Şeker , Hasan Pınar Nedim Mutlu , Hayati Bat

Abstract

Tomato is one of the important vegetables produced in the world, and China, USA and Turkey are among the important producers in world production. Diseases and pests are one of the most important problems in tomato as in other species. Breeding for disease resistance has been an important objective in tomato improvement. When preparing new breeding programs, genetic materials from different backgrounds are needed. However, the genes for resistance to diseases and pests may vary according to the tomato production regions. In order to obtain the desired resistance in breeding programs, it is important to know which resistance is needed for which region. From this point of view, in this study, it is aimed to determine the distribution of disease and pest resistance of the lines developed from tomato varieties produced in Turkey, USA and Jordan. In the materials selected from Turkey in 2014, disease resistance other than Verticillium was rarely found, while Sw-5 and Ph3 genes were found in 2017 and 2019. On the other hand, it was determined that the majority of genetic materials originating from USA and Jordan had Ve, FF, Mi1-2, Pto, Tm-2, Ty-3, Ph3 and SW-5 genes. The findings show that it can be taken into account in developing disease and pest resistant varieties for different production regions.

Keywords

Tomato , Molecular markers , MAS

References

• Acciarri N, Rotino GL, Tamietti G, Valentino D, Voltattorni S and Sabatini E (2007). Molecular markers for Ve1 and Ve2: Verticillium resistance genes from Italian tomato germplasm. Plant Breeding, 126, 617-621.
• Arens P, Mansilla C, Deinum D Cavellini, L, Moretti A, Rolland S, ... and Vosman B (2010). Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing. Theoretical and applied genetics, 120, 655-664.
• AVDRC (1994). AVDRC Progress Report 1993, Asian Vegetable Research and Development Center, Shanhua, Tainan, Taiwan pp.201–203
• Barone A (2004). Molecular Marker Assisted Selection for Resistance to Pathogens in Tomato. MAS in Plants http://www.fao.org/biotech/docs/Barone.pdf.
• Birch P R J and Whisson S, (2001). Phytophthora infestans enters the genomics era. Mol Plant Pathol, 2 pp.257–263.
• Bülbül C, Şimşek D, Mutlu N and Işık S (2022). Using Multi-Disease Resistance in Tomato Cultivation for Sustainable Agriculture. Proceeding Book, 140.
• Devran Z Ü and Elekçioğlu H (2004). The screening of F2 plants for the Root-Knot nematode resistance gene, Mi by PCR in Tomato. Turkish Journal of Agriculture and Forestry, 253-257.
• Doyle J J and Doyle J L (1990). Isolation of plant DNA from fresh tissue. Focus, 12:13-15.
• El Mohtar, C A, Atamian H S, Dagher R B, Abou-Jawdah Y, Salus M S, Maxwell D P (2007). Marker-assisted selection of tomato genotypes with the I-2 gene for resistance to Fusarium oxysporum f. sp. lycopersici race 2. Plant Disease, 91:758-762.
• FAO (2021). Retrieved in May, 9, 2023 from https://www.fao.org/faostat/en/#data/QCL
• Foolad M (2007). Genome mapping and molecular breeding of tomato. Int J Plant Genomics, 2007:52.
• Foolad M R, Merk H L and Ashrafi H (2008). Genetics, genomics and breeding of late blight and early blight resistance in tomato. Critical Reviews in Plant Sciences, 27(2), pp.75-107.
• Kamoun S and Smart C D (2005). Late blight of potato and tomato in the genomics era. Plant Dis, 89, pp.692–699.
• Kıymacı G, Arı B Ç, Uncu A T, Uncu, A Ö, Issı, N and Türkmen Ö (2023). Determination of Resistance Levels of Selected Tomato Genotypes to Meloidogyne incognita, Tomato Yellow Leaf Curling Virus (TYLCV) Verticillium Wilt, Fusarium oxysporum radicis, Fusarium Wilt, Tomato Spotted Wilt Virus (TSWV). Selcuk Journal of Agriculture and Food Sciences, 37(1), 86-94.
• Medina-Filho H, Stevens M (1980) Tomato breeding for nematode resistance: survey of resistant varieties for horticultural characteristics and genotype of acid phosphatase. Acta Hort, 100:383–391.
• Moreau P, Thoquet P, Olivier J Laterrot,H and Grimsley N (1998). Genetic mapping of Ph-2, a single locus controlling partial resistance to Phytophthora infestans in tomato. Molecular Plant-Microbe Interactions, 11(4), pp.259-269.
• Panthee D R and Foolad M R (2011). A reexamination of molecular markers for use in marker-assisted breeding in tomato. Euphytica, DOI 10.1007 /s10681-011-0544-5.
• Pedersen W L and Leath S (1988). Pyramiding major genes for resistance to maintain residual effects. Annual Review of Phytopathology, 26(1), pp.369-378.
• Peirce L C (1971). Linkage tests with Ph conditioning resistance to race 0, Phytophthora infestans. Tomato Genet. Co-op. Rep., 21, pp.30.
• Ren Z, You Z, Munir S, Zhang Y, Li H, Zhang J, ... and Ye Z (2019). Development of a highly specific co-dominant marker for genotyping the Ph-3 (tomato late blight resistance) locus by comparing cultivated and wild ancestor species. Molecular Breeding, 39, 1-8.
• Tanksley S D (1983). Gene mapping. In Developments in Plant Genetics and Breeding Vol. 1, pp. 109-138 Elsevier.
• Tanksley S D, Ganal M W, Prince J P, Devicente M C, Bonierbale M W, Broun P, Fulton T M, Giovannoni J J, Grandillo S, Martin G B, Messeguer R, Miller J C, Miller L, Paterson A H, Pineda O, Roder M S,
• Wing R A, Wu W, Young N D (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics, 132:1141–1160.
• Shekasteband R, Hutton S F and Scott J W (2015). Designing new DNA markers and determining the effective size of Ph-2 and Ph-3 introgressions for late blight resistance stacking purposes in tomato. TGC REPORT, 65.
• Vidavski, F, Czosnek, H, Gazit S, Levy D and Lapidot M (2008). Pyramiding of genes conferring resistance to Tomato yellow leaf curl virus from different wild tomato species. Plant Breeding, 127(6), pp.625-631.
• Yang W and D M Francis (2005). Marker-assisted selection for combining resistance to bacterial spot and bacterial speck in tomato. Journal of the American Society for Horticultural Science, 130, pp.716–721.
• Zhang J and Panthee D R (2021). Development of codominant SCAR markers to detect the Pto, Tm22, I3 and Sw5 genes in tomato (Solanum lycopersicum). Plant Breeding, 140(2), 342-348.