ankara ecz. fak. derg.

Journal of Faculty of Pharmacy of Ankara University

1015-3918 2564-6524

Ankara Üniversitesi

10.33483/jfpau.1529091

Pharmaceutical Biochemistry

Eczacılık Biyokimyası

KÜÇÜK HÜCRELİ VE KÜÇÜK HÜCRELİ OLMAYAN AKCİĞER KANSERLERİNDE ROL OYNAYAN MİRNA’LARIN BİYOİNFORMATİK ANALİZLERLE KARŞILAŞTIRILMASI

THE COMPARISON OF MICRORNAS INVOLVED IN SMALL CELL LUNG CANCER AND NON-SMALL CELL LUNG CANCER THROUGH BIOINFORMATICS ANALYSES

https://orcid.org/0000-0003-4683-074X

Hekmatshoar

Yalda

ALTINBAS UNIVERSITY, SCHOOL OF MEDICINE

https://orcid.org/0000-0002-5499-5168

Karadağ Gürel

Aynur

USAK UNIVERSITY, SCHOOL OF MEDICINE

https://orcid.org/0009-0004-1408-8944

Aydoğan

Adem

USAK UNIVERSITY, SCHOOL OF MEDICINE

05 19 2025

49 2 284 300 08 06 2024 12 10 2024

1971

Ankara Üniversitesi Eczacılık Fakültesi Dergisi

Amaç: Akciğer kanseri, dünya çapında karsinomla ilişkili ölümlerin önemli bir nedenidir. Küçük hücreli ve küçük hücreli olmayan 2 ana akciğer kanseri türü bulunmaktadır. İyi prognozun elde edilmesi, hedefe yönelik tedavinin geliştirilmesi ve potansiyel biyobelirteçleri belirlenmesi amacıyla yeni yöntemler bulmak, akciğer kanserinin klinik etkinliğini geliştirilmesi için büyük önem taşımaktadır. Çalışmanın amacı 2 alt tipte farklı ifade olan miRNA’ları bularak patogenezi ve potansiyel moleküler belirteçlerini incelemektir.Gereç ve Yöntem: miRNA verisi içeren GSE19945 ve GSE135918 nolu veri seti GEO veri tabanından indirilmiştir. GEO2R çevrimiçi analiz aracı ile P<0.05 ve log kat değişimi |(FC)|≥ 1 alınarak analiz edilmiştir. Farklı ifade edilen miRNA’ların hedef genleri tanımlanmıştır. Cytoscape PPI kullanılarak ağ görselleştirme ve modül tanımlaması yapılmıştır. miRNA hedef genlerinden 3 adet seçilerek genlerin validasyonu akciğer kanseri hücre hattı A549'da yapılmıştır. Sonuç ve Tartışma: 17 ifadesi azalan ve 2 ifadesi artan ortak miRNA’lar arasında hsa-miR-1249, hsa-miR-326, hsa-let-7c, hsa-miR-199a-5p, hsa-miR-940, hsa-miR-139-3p, hsa-miR-142-3p, hsa-miR-142-5p, hsa-miR-455-5p, hsa-miR-146b-5p,hsa-miR-152hsa-miR-133b, hsa-miR-498, hsa-miR-199b-5p, hsa-miR-140-3p, hsa-miR-203 ve hsa-miR-139-5p yer almaktadır. miRNA’ların moleküler fonksiyonlarının ve sinyal yollarının tanımlanması, 2 kanser tipinin moleküler mekanizmalarının mevcut anlayışını derinleştirebilir ve, tedavi seçeneklerinin geliştirilmesine katkıda bulunabilir.

Objective: Lung cancer is a major cause of cancer-related deaths worldwide. There are two main types of lung cancer, small cell and non-small cell. Finding new methods for achieving a good prognosis, developing targeted therapy and identifying potential biomarkers is crucial for improving the clinical efficacy of lung cancer. The aim of this study was to investigate the pathogenesis and potential molecular markers by finding differentially expressed miRNAs in 2 subtypes of lung cancer. Materials and Methods: The datasets GSE19945 and GSE135918 containing miRNA data were downloaded from the GEO database. Analyzed with GEO2R online analysis tool with P<0.05 and log fold change |(FC)|≥ 1. Target genes of differentially expressed miRNAs have been identified. Network visualisation and module identification were performed using Cytoscape PPI. Three of the miRNA target genes were selected and validation of the genes was performed in the non-small cell lung cancer cell line A549. Results and discussion: 17 common miRNAs with decreased expression and 2 with increased expression include hsa-miR-1249, hsa-miR-326, hsa-let-7c, hsa-miR-199a-5p, hsa-miR-940, hsa-miR-139-3p, hsa-miR-142-3p, hsa-miR-142-5p, hsa-miR-455-5p, hsa-miR-146b-5p,hsa-miR-152hsa-miR-133b, hsa-miR-498, hsa-miR-199b-5p, hsa-miR-140-3p, hsa-miR-203 and hsa-miR-139-5p. Defining the molecular functions and signaling pathways of miRNAs may deepen the current understanding of the molecular mechanisms of the 2 cancer types and contribute to the development of treatment options.

Akciğer kanseri biyoinformatik analiz küçük hücreli akciğer kanseri küçük hücreli olmayan akciğer kanseri miRNA

Bioinformatics analysis lung cancer microRNA non-small cell lung cancer small cell lung cancer

TÜBİTAK, Uşak Üniversitesi BAP

1919B012218259 , 2024/LP001

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