CAMSI KARBON VE BOR KATKILI ELMAS ELEKTROTLAR KULLANARAK OTİLONYUM BROMÜRÜN ELEKTROKİMYASAL DEĞERLENDİRİLMESİ

Öz

Amaç: Döngüsel (CV) ve diferansiyel darbe (DPV) voltametrik teknikler kullanılarak, otilonyum bromürün (OTB) elektrokimyasal araştırması, bor katkılı elmas elektrotlarda (BDDE) ve camsı karbon elektrotlarda (GCE) geniş bir pH aralığında (0.3–12) gerçekleştirildi. OTB'nin tipik elektrokimyasal davranışının, çalışan elektrot tipine ve pH'a bağlı olduğu tespit edildi. Bu araştırmanın amacı, tampon çözeltilerde OTB'yi ölçmek için yepyeni bir elektroanalitik teknik sağlamaktır. Gereç ve Yöntem: 10 ml kapasiteli tipik tek bölmeli üç elektrotlu hücre, tüm çalışmalarda bir BDDE ve GCE çalışma elektrotu, bir platin tel karşıt elektrot ve bir Ag/AgCl referans elektrotu ile birlikte kullanıldı. Elektrokimyasal ölçümleri gerçekleştirmek için NOVA 1.8 yazılımı ve bir AUTOLAB 204 potansiyostat/galvanostat kullanıldı. Sonuç ve Tartışma: "Antispazmodikler" (spazm ve kramp giderici) adı verilen bir ilaç sınıfına ait olan OTB'nin elektrokimyasal davranışı, öncelikle hassas bağırsak sendromunu (IBS) ve hareketlilik sorunları, ağrılı bağırsak spazmları ve şişkinliği (şişme) ile karakterize edilen diğer gastrointestinal rahatsızlıkları tedavi etmek için kullanılır. ve göbek bölgesinde şişkinlik), GCE ve BDDE'de 0.1 M H2SO4'te incelenmiştir. Geniş bir pH aralığı (pH 0.3-12.0) boyunca çeşitli pH seviyelerinde CV araştırmaları yapılarak elektrooksidasyon mekanizması da araştırıldı. Mekanizmanın anlaşılması, difüzyonun her iki elektrot için de kontrol edildiğini ortaya koyan tarama hızı araştırmalarıyla desteklendi. Önerilen yöntem, optimal koşullar altında OTB'yi belirlemek için başarıyla kullanılmıştır.

Anahtar Kelimeler

• Bor katkılı elmas elektrot
• camsı karbon elektrot
• diferansiyel puls voltammetrisi
• ilaç analizi
• Otilonyum bromür

ELECTROCHEMICAL INVESTIGATION OF OTILONIUM BROMIDE USING BORON-DOPED DIAMOND AND GLASSY CARBON ELECTRODES

Öz

Objective: Using cyclic (CV) and differential pulse (DPV) voltammetric techniques, the electrochemical research of otilonium bromide (OTB) was carried out over a wide pH range (0.3–12) at glassy carbon electrodes (GCE) and boron-doped diamond electrodes (BDDE). The typical electrochemical behavior of OTB was identified as being dependent on the type of working electrode and pH. This research aims to provide a brand-new electroanalytical technique for measuring OTB in buffer solutions. Material and Method: All experiments employed the typical three-electrode cell of 10 ml capacity in conjunction with a platinum wire counter electrode, a BDDE and GCE working electrode, and an Ag/AgCl reference electrode. NOVA 1.8 software and an AUTOLAB 204 potentiostat/galvanostat were used for electrochemical measurements. Result and Discussion: The electrochemical behavior of OTB, which belongs to a class of drugs called 'antispasmodics' (spasm and cramps reliever), primarily used to treat irritable bowel syndrome (IBS), and other gastrointestinal conditions characterized by motility problems, painful bowel spasms and distension (swelling and bloating in the belly area), was examined in 0.1 M H2SO4 at BDDE and GCE. The electrooxidation mechanism was also investigated by conducting CV investigations at various pH levels throughout a broad pH range (pH 0.3-12.0). Understanding the mechanism was aided by scan rate investigations, which revealed that diffusion was controlled for both electrodes. The proposed technique was successfully used to determine OTB under optimal conditions.

Anahtar Kelimeler

• Boron-doped diamond electrode
• differential pulse voltammetry
• drug assay
• glassy carbon electrode
• Otilonium bromide

Kaynakça

1. 1. Zhou, L., She, P., Tan, F., Li, S., Zeng, X., Chen, L., Luo, Z., Wu, Y. (2020). Repurposing antispasmodic agent Otilonium bromide for treatment of Staphylococcus aureus infections. Frontiers in Microbiology, 11, 1-12. [CrossRef]
2. 2. Clavé, P., Tack, J. (2017). Efficacy of otilonium bromide in irritable bowel syndrome: A pooled analysis. Therapeutic Advances in Gastroenterology, 10, 311-322. [CrossRef]
3. 3. Battaglia, G., Morselli-Labate, A.M., Camarri, E., Francavilla, A., De Marco, F., Mastropaolo, G., Naccarato, R. (1998). Otilonium bromide in irritable bowel syndrome: A double-blind, placebo-controlled, 15-week study. Aliment. Pharmacology & Therapeutics, 12, 1003-1010. [CrossRef]
4. 4. Zaki, S.A., Helal, M.E., Rashid, A. (2022). Cardiovascular toxicity due to Otilonium bromide overdose: A case report. Journal of Emergency Medicine, 62, e47-e50. [CrossRef]
5. 5. Vire, J.C., Kauffmann, J.M. (1994). Trends in Electrochemistry in Drug Analysis. Current Top Electrochem. From https://rseqelectroquimica.files.wordpress.com/2021/07/2021-paris-libro-de-resumenes.pdf. Accessed date: 12.08.2023.
6. 6. Brett, C.M.A., Brett, A.M.O. (1993). Electrochemistry: Principles, methods, and applications, p.427.
7. 7. Pletcher, D., Greff, R., Peat, R., Peter, L.M., Robinson, J. (2021). Instrumental Methods in Electrochemistry, Woodhead Publishing Limited.
8. 8. Bagotsky, V.S. (2006). Fundamentals of Electrochemistry, Fundam. Electrochem, 2nd Edition, 51-60. From https://www.wiley.com/en-us/Fundamentals+of+Electrochemistry%2C+2nd+Edition-p-978047170 0586. Accessed date: 27.11.2021.

9. 9. Ambrosi, A., Chua, C.K., Bonanni, A., Pumera, M. (2014). Electrochemistry of graphene and related materials. Chemical Reviews, 114, 7150-7188. [CrossRef]
10. 10. Dekanski, A., Stevanović, J., Stevanović, R., Nikolić, B.Ž., Jovanović, V.M. (2001). Glassy carbon electrodes: I. Characterization and electrochemical activation. Carbon N. Y, 39, 1195-1205. [CrossRef]
11. 11. Luong, J.H.T., Male, K.B., Glennon, J.D. (2009). Boron-doped diamond electrode: Synthesis, characterization, functionalization and analytical applications. Analyst, 134, 1965-1979. [CrossRef]
12. 12. Chailapakul, O., Siangproh, W., Tryk, D.A. (2006). Boron-doped diamond-based sensors: A review. Sensor Letters, 4, 99-119. [CrossRef]
13. 13. Ermer, J., Miller, J. (2006). Method validation in pharmaceutical analysis: A guide to best practice, Wiley-VCH, 2nd Edition. From https://books.google.com/books?hl=en&lr=&id=HbBlyvlRgwkC&oi=fnd&pg =PR5&ots=nWHqAOz4Ij&sig=0BmCZoEKSznXve0cjXBJE_mdUQ4. Accessed date: 18.12.2022.
14. 14. Ozkan, S.A., Kauffmann, J.M., Zuman, P. (2015). Electroanalysis in biomedical and pharmaceutical Sciences, Springer. [CrossRef]
15. 15. Santoni, G., Fabbri, L., Mura, P., Renzi, G., Gratteri, P., Pinzauti, S. (1991). Simultaneous determination of otilonium bromide and diazepam by high performance liquid chromatography. International Journal of Pharmaceutics, 71(1-2), 1-5. [CrossRef]
16. 16. Zhao, Y.R., Ding, L., Fan, H.W., Yu, Y., Qi, X.M., Leng, Y., Rao, Y.K. (2010). Determination of the unstable drug otilonium bromide in human plasma by LC–ESI-MS and its application to a pharmacokinetic study. Journal of Chromatography B, 878(28), 2896-2900. [CrossRef]
17. 17. Mannucci, C., Bertini, J., Cocchini, A., Perico, A., Salvagnini, F., Triolo, A. (1993). High‐performance liquid chromatographic method for assay of otilonium bromide, diazepam, and related compounds in finished pharmaceutical forms. Journal of Pharmaceutical Sciences, 82(4), 367-370. [CrossRef]
18. 18. Furlanetto, S., Orlandini, S., Massolini, G., Faucci, M.T., La Porta, E., Pinzauti, S. (2001). Optimisation and validation of a capillary electrophoresis method for the simultaneous determination of diazepam and otilonium bromide. Analyst, 126(10), 1700-1706. [CrossRef]
19. 19. Morelli, B. (1997). Determination of diazepam and otilonium bromide in pharmaceuticals by ratio-spectra derivative spectrophotometry. Fresenius' Journal of Analytical Chemistry, 357, 1179-1184. [CrossRef]
20. 20. Mannucci, C., Bertini, J., Cocchini, A., Perico, A., Salvagnini, F., Triolo, A. (1992). Simultaneous determination of otilonium bromide and diazepam by first-derivative spectroscopy. Journal of pharmaceutical sciences, 81(12), 1175-1177. [CrossRef]