Lipopolisakkarit ile indüklenen nöroinflamasyon modelinde köşe dönme testinde dönüş tercihleri ve sağ-sol beyin hemisferlerinde proinflamatuar sitokinlerin durumu

Year 2025, Volume: 8 Issue: 4 , 640 - 645 , 30.07.2025

Hasan Çalışkan

https://doi.org/10.32322/jhsm.1691744

https://izlik.org/JA58PS52UL

Abstract

Amaç: Nöroinflamasyon, sinir sistemi içindeki enflamatuar bir tepki olarak tanımlanır. Bu çalışmada, lipopolisakkarit ile indüklenen nöroinflamasyon modelinde köşe dönme testinde sıçanların davranışları ve sağ/sol hemisferler arasında proinflamatuar sitokinlerin dağılımı incelenmiştir. Ayrıca, lokomotor aktivite, anksiyete benzeri davranışlar ve stresle ilişkili harder bezi salgısı da analiz edilmiştir.

Yöntemler: 12 yetişkin erkek Wistar Albino sıçan kontrol (n=6) ve nöroinflamasyon (n=6) gruplarına ayrıldı. Nöroinflamasyon grubuna tek doz 2 mg/kg intraperitoneal LPS, kontrol grubuna ise 1 ml/kg fizyolojik salin uygulandı. LPS uygulamasından 6 saat sonra 5 dakikalık açık alan testi yapıldı. Köşe dönüş testi protokolü LPS uygulamasından 6 ve 24 saat sonra uygulandı. Denekler, LPS uygulamasından 24 saat sonra öldürüldü. TNF-a ve IL-1beta, prefrontal korteks, striatum ve serumda analiz edildi. Tüm prosedürler Balıkesir Üniversitesi Deney Hayvanları Etik Kurulu'nun onayıyla (onay numarası 2023/10-5) gerçekleştirildi. Sonuçlar, öğrencilerin t-testi kullanılarak analiz edildi.

Bulgular: Açık alan testinde, lokomotor aktivite azalırken (p<0,0001), anksiyete benzeri davranışlar artmıştır (p<0,05-p<0,0001). Harderian bezi salgısı, nöroinflamasyon grubunda 4-5 puanlık bir artışla anlamlı olarak artmıştır (p<0,0001). Köşe dönme testinde kontrol ve LPS grupları arasında fark gözlenmedi (p>0,05). TNF-alfa ve IL-1beta, kontrol grubuna kıyasla önemli ölçüde arttı (p<0,01-p<0,0001). Sağ hemisferler arasında proinflamatuar sitokinlerde fark yoktu (p>0,01).
Sonuç: Nöroinflamasyon grubunda anksiyete benzeri davranışlar ve proinflamatuar sitokinler artmıştır. Göz ve burun çevresinde stresle ilişkili lekelenme artmıştır. Köşe dönme testine göre, anlamlı bir fark gözlenmemiştir. Nörolojik deneylerde hemisferlere verilen hasarı inceleyen köşe dönme testi ile nöroinflamasyon modellerinde daha fazla çalışma yapılması gerekmektedir.

Keywords

aksiyete benzeri davranışlar , köşe dönme testi , nöroinflamasyon , açık alan testi , sağ-sol hemisfer farkları

Turning preferences in the corner turning test and the status of proinflammatory cytokines in the right-left brain hemispheres in the lipopolysaccharide-induced neuroinflammation model

https://izlik.org/JA58PS52UL

Abstract

Aims: Neuroinflammation is described as an inflammatory response within the nervous system. The study aimed to investigate the behavior of rats in the corner turning test and the distribution of proinflammatory cytokines between the right/left hemispheres in the neuroinflammation model induced by lipopolysaccharide. Furthermore, locomotor activity, anxiety-like behaviors, and stress-related harderian gland secretion were also analyzed.
Methods: 12 adults male Wistar albino rats were divided into control (n=6) and neuroinflammation animals (n=6). The neuroinflammation group received a single dose of 2 mg/kg intraperitoneally LPS, and 1 ml/kg physiological saline was administered to the control group. Six hours after LPS administration, open field test was performed for 5 minutes. The corner rotation test protocol was performed 6 and 24 hours after LPS administration. Subjects were sacrificed 24 hours after LPS administration. TNF-a and IL-1beta were analyzed in the prefrontal cortex, striatum, and serum. All procedures were conducted with the approval of the Balıkesir University Experimental Animals Ethics Committee (Date: 22.11.2023, Decision No: 2023/10-5). Results were analyzed using the students’ T test.
Results: In the open field test, locomotor activity decreased (p<0.0001), while anxiety-like behaviors increased (p<0.05-p<0.0001). Harderian gland secretion significantly increased by 4-5 points in the neuroinflammation group (p<0.0001). No difference was observed between the control and LPS groups in the corner-turning test (p>0.05). TNF-alpha and IL-1beta significantly increased compared to the control group (p<0.01-p<0.0001). There was no difference in proinflammatory cytokines between the right hemispheres (p>0.01).
Conclusion: In the neuroinflammation group, anxiety-like behaviors and proinflammatory cytokines increased. Stress-related spotting around the eyes and nose increased. According to the corner-turning test, no significant difference was observed. More studies are needed in neuroinflammation models with the corner-turning test, which examines damage to the hemispheres in neurological experiments.

Keywords

Anxiety-like behaviors , corner-turning test , neuroinflammation , open-field test , right-left hemisphere differences

Ethical Statement

The Animal Experiments Local Ethics Committee of Balıkesir University approved this study (Decision No: 2023/10-5).

Thanks

We want to thank Dr. Vet. Dr. Mustafa Hilmi Yaranoğlu and Health Technician Mehmet Bozdemir for their help in providing animals.

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