Assessment of the performance of CHROMagar Candida media in identification of candida strains

Yıl 2018, , 50 - 53, 30.09.2018

Sami Kınıklı Esra Kaya Kılıç Kader Arslan Salih Cesur , Çiğdem Ataman Hatipoğlu Serap Yağcı Bedia Dinç

https://doi.org/10.32322/jhsm.440907

Öz

Objective: The treatment of Candida infections differs according to Candida species. The identification by routine and conventional methods can take several days. For this reason, rapid diagnosis of Candida species is important for initiating an effective antifungal therapy.

Material and Method: Sixty one Candida strains included in the study were evaluated at 24th and 48th hours after they were cultivated on CHROMagar Candida medium and incubated under appropriate conditions in accordance with the manufacturer's recommendations.

Results: When CHROMagar Candida medium and reference methods were compared in the study, it was found that CHROMagar Candida and referance method were  detected the same species in 36 of the 61 samples (40.9%). Candida species were produced in CHROMagar Candida medium in 25 samples (59.1%) but the identification of species could not be made.

Conclusion: The CHROMagar Candida medium  allows the discrimination of  Candida parapsilosis species as well as Candida albicans according to the germe tube test, but does not allow the discrimination of other  Candida species. It also has disadvantages of having problems due to different colors and high cost.

Anahtar Kelimeler

Candida species, rapid diagnosis, chromogenic medium

Candida suşlarının tanımlanmasında CHROMagar Candida besiyerinin performansının değerlendirilmesi

Öz

Amaç: Candida enfeksiyonlarının
tedavisi Candida türlerine göre
farklılık göstermektedir. Rutin ve klasik yöntemlerle identifikasyon birkaç
günü alabilmektedir. Bu nedenle, etkili antifungal tedavinin başlanabilmesi
için Candida türlerinin hızlı tanısı
önemlidir.

Gereç ve Yöntem: Çalışmaya dahil edilen  61 Candida
suşu  CHROMagar Candida besiyerine ekilip üreticinin önerileri doğrultusunda uygun
şartlarda inkübe edildikten sonra,  24.
ve 48. saatlerde değerlendirildi.

Bulgular: Çalışmada CHROMagar Candida
besiyeri ile referans yöntemler karşılaştırıldığında, CHROMagar Candida 
ile 61 örneğin 36’sında (%40,9) 
referans yöntemle aynı tür tayini saptanmıştır. Yirmi beş örnekte
(%59,1) ise CHROMagar Candida  besiyerinde Candida türleri üremiş ancak tür tayini yapılamamıştır.

Sonuç: CHROMagar Candida besiyeri germ tüp testine göre Candida albicans‘ın
yanısıra, Candida parapsilosis türlerinin ayrımına imkan
sağlamasına rağmen, diğer Candida türlerin
ayrımına imkan sağlamaması, farklı renkler nedeniyle sorunlar yaşanması ve
maliyetinin yüksek olması dezavantajlarıdır.    

Anahtar Kelimeler

Candida türleri, hızlı tanı, kromojenik besiyeri

Kaynakça

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