The effect of resveratrol on toxicity caused by cisplatin in rats with experimentally created diabetes by streptozotocin

Yıl 2022, , 124 - 130, 17.01.2022

Bahattin Bulduk , Gokhan Oto , Nizamettin Günbatar , Mehmet Bulduk , Yılmaz Koçak , Sadi Elasan

https://doi.org/10.32322/jhsm.999224

Cited By: 2

Öz

Aim: In our study, the therapeutic effect of resveratrol against the toxicity of cisplatin in rats with experimental diabetes mellitus with streptozotocin was investigated.
Material and Method: 64 rats were used in the study. 8 groups were randomly formed, with 8 rats in each group. Group 1 was determined as the control group. Group 2 (STZ) was injected with 60 mg/kg streptozotocin intraperitoneally (ip) on the first day to induce diabetes. Group 3 (RES) was given 100 mg/kg of resveratrol orally every day. Group 4 (SIS), a single dose of cisplatin 7 mg/kg (ip) was administered 3 days later. Group 5 (STZ+RES), group 6 (STZ+SIS), group 7 (RES+SIS) and group 8 (STZ+SIS+RES) were determined.
Results: While there was weight gain in the control and RES groups during the experiment, the STZ and STZ + SIS groups showed a significant decrease in body weights of the rats. In the groups given streptozotocin and cisplatin together with resveratrol, there was no decrease in body weight, but a small increase was observed. In groups with increased blood glucose values with streptozotocin, these values were found to have dropped significantly with resveratrol. The TAS level has increased significantly in groups RES, STZ+RES, SIS+RES and STZ+SIS+RES according to the control group; no significant difference has been found in the other groups compared to the control group. While the AST level was significantly higher in the STZ, SIS and STZ+SIS groups compared to the control group, the ALT level was found to be significantly higher in the STZ and STZ+SIS groups compared to the control group. Creatinine was found to be significantly higher in SIS, STZ+SIS, RES+SIS and STZ+SIS+RES groups compared to the control group. The SIS group and RES+SIS and STZ+SIS+RES groups were compared, the decrease in the RES+SIS and STZ+SIS+RES groups was statistically significant. QT (ms) values increased significantly in the STZ and STZ+SIS groups compared to the control group, but there was no significant difference in the other groups. According to the control group, the heart rate per minute was found to be significantly lower in the STZ and STZ+SIS groups.
Conclusion: As a result, it was seen that the use of resveratrol would be effective in reducing the increased glucose levels in the treatment of diabetes and in the treatment of possible complications.

Anahtar Kelimeler

Diabetes, Cispilatin, Resveratrol, ECG

Destekleyen Kurum

Van Yüzüncü Yıl University Scientific Research Projects Coordination Department

Proje Numarası

2015-VSYO-B257

Kaynakça

• Bulduk B. Diabetes mellitus ve oral atidiyabetikler, C. (Ed). Evereklioğlu içinde, Sağlık Bilimlerinde Araştırma ve Değerlendirmeler 1. baskı, Ankara: Gece Kitaplığı; 2021.
• Hung LM, Chen JK, Huang SS, Lee RS, Su MJ. Cardioprotective effect of resveratrol, a natural antioxidant derived from grapes. Cardiovasc Res 2000; 47: 549-55.
• Arany I, Safirstein RL. Cisplatin nephrotoxicity. Semin Nephrol 2003; 23: 460–4.
• Astolfi L, Ghiselli S, Guaran V, et al. Correlation of adverse effects of cisplatin administration in patients affected by solid tumours: A retrospective evaluation Oncol Rep 2013; 29: 1285-92.
• Yadav S, Vats V, Dhunnoo Y, Grover J.K. Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats. J Ethnophamacol 2002; 82: 111-6.
• Pari L, Latha L. Protective role of Scopari dulcis plant extract on brain antioxidant status and lipidperoxidation in STZ diabetic male wistar rats. BMC Compliment Altern Med 2004; 4:16-24.
• Bulduk B, Kılınç D. Günlük Crataegus oxyacatha (Alıç) uygulamasının ratlarda EKG değerlerine etkisi. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 2013; 24: 77-81.
• Heydari I, Radi V, Razmjou S, Amiri A. Chronic complications of diabetes mellitus in newly diagnosed patients. Int J Diabetes Mellitus 2010; 2: 61–3.
• Kikkawa R. Chronic complications in diabetes mellitus. Br J Nutr 2000; 2: 183-5.
• Susztak K, Raff AC, Schiffer M, Bottinger EP. Glucose- induced reactive oxygen species cause apoptosis of podocytes and podocytedepletion at the onset of diabetic nephropathy. Diabetes 2006; 55: 225-33.
• Parker AB, Yusuf S, Naylor CD. The relevance of subgroup-specific treatment effects: the Studies of left ventricular dysfunction (SOLVD) revisited. Am Heart J 2002; 144: 941-7.
• Kurçer Z, Karaoğlu D. Deneysel diyabet modellerinde alloksan ve streptozotosin kullanımı. Türk Jem 2012;16: 34-40.
• Green A, Sjølie AK, Eshøj O. Trends in the epidemiology of IDDM during 1970–2020 in Fyn County, Denmark. Diabetes Care 1996;19: 801-6.
• Kanter M, Aktas C, Erboga M. Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin-induced diabetic rat testis. Food Chem Toxicol 2012; 50: 719-25.
• Khaki A, Fathiazad F, Nouri M, Khaki A, Maleki NA, Khamnei HJ, Ahmadi P. Beneficial effects of quercetin on sperm parameters in streptozotocin‐induced diabetic male rats. Phytother Res 2010; 24: 1285-91.
• Sharma S, Anjeneyulu M, Kulkarni SK, Chopra K. Resveratrol, a polyphenolic phytoalexin, attenuates diabetic nephropathy in rats. Pharmacology 2006; 76: 69–75.
• Su HC, Hung LM, and Chen JK. Resveratrol, a red wine antioxidant, possesses an insülin-like effect in streptozotocin-induced diabetic rats. Am J Physiol Endocrinol Metab 2006; 290: 1339-46.
• Chen KH, Hung CC, Hsu HH, Jing YH, Yang CW, Chen JK. Resveratrol ameliorates early diabetic nephropathy associated with suppression of augmented TGF-β/smad and ERK1/2 signaling in streptozotocin-induced diabetic rats. Chemico Biological Interactions 2011;15: 45-53.
• Tzong-Cherng C, Chen WP, Chi T-Li, et al. Phosphatidylinositol-3-kinase is Involved in the antihyperglycemic effect induced by resveratrol in streptozotocin- induced diabetic rats. Life Sci 2007; 80: 1720-13.
• Nihei T, Miura Y, Yagasaki K. Inhibitory effect of resveratrol on proteinuria, hypoalbuminnemia and hyperlipidemia in nephritic rats. Life Sci 2001; 68: 2845-52.
• Chi TC, Chen WP, Chi TL, et al. Phosphatidylinositol-3-kinase is involved in the antihyperglycemic effect induced by resveratrol in streptozotocin-induced diabetic rats, Life Sci 2007; 80: 1713–20.
• Işık A, Koca S. Behçet hastaliğinda total antioksidan cevap ve oksidatif stres. Fırat Üniversitesi Sağlık Bilimleri Derg 2006; 20: 415-21.
• Rodrigo R, Bosco C. Oxidative stress and protective effects of polyphenols: comparative studies in human and rodent kidney. A review. Comp Biochem Physiol Part C: Toxicol Pharmacol 2006; 142: 317-27.
• Kasdallah-Grissa A, Mornagui B, Aouani E, et al. Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver. Life Sci 2007; 20: 1033-9.
• Mıkulskı D, Gornıak R, Molsklı M. A theoretical study of the structure-radical scavenging activity of trans-resveratrol analogues and cisresveratrol in gas phase and water environment. Eur J Med Chem 2010; 45: 1015-27.
• Carrızzo A, Forte M, Damato A, et al. Antioxidant effects of resveratrol in cardiovascular, cerebral and metabolicdiseases. Food Chem Toxicol 2013; 61: 215-26.
• Gambını J, Ingles M, Olaso G, Lopez-Grueso R, Bonet-Costa V, Gımeno-Mallench L. Properties of Resveratrol: In vitro and in vivo studies about metabolism, bioavailability, and biological effects in animal models and humans. Oxid Med Cell Longev 2015; 2015: 837042.
• Leonard SS, Xia C, Jiang BH, et al. Resveratrol scavenges reactive oxygen species and effects radicalinduced cellular responses. Biochem Biophys Res Commun 2003; 309: 1017-26.
• Juhasz B, Varga B, Gesztelyi R, et al. Resveratrol: a multifunctional cytoprotective molecule. Curr Pharm Biotechnol 2010; 11: 810-8.
• Bulduk M, Oto G, Ozdemir H, Demirel-Yilmaz E. The effect of resveratrol therapy on the vascular responses caused by chronic fluorosis. Fluoride 2020; 53: 23-39.
• Pandey KB, Rizvi SI. Anti-oxidative action of resveratrol: Implications for human health, Arabian J. Chem 2010; 4: 293-6.
• Şehirli O, Tozan A, Omurtag GZ, et al. Protective effect of resveratrol against naphthalene-induced oxidative stress in mice. Ecotoxicol and Environ Saf 2008; 71: 301–8.
• Yulug E, Türedi S, Karagüzel E, Kutlu O, Mentese A, Alver A. The short term effects of resveratrol on ischemia–reperfusion injury in rat testis. J Pediatr Surg 2014; 49: 484-9.
• Bozkurt N. DMBA ile oluşturulan rat karaciğer hasarında resveratrol ve pekmezin karaciğer enzimleri ve oksidatif stres parametreleri üzerine etkilerinin araştırılması. İnönü Üniversitesi Sağlık Bilimleri Enstitüsü Yüksek Lisans Tezi 2014.
• Juan ME, Vinardell MP, Planas JM. The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful. J Nutr 2002; 132: 257-60.
• Ghosh S, Bhattacharyya S, RashidK, Sil PC. Curcumin protects rat liver from streptozotocin-induced diabetic pathophysiology by counteracting reactive oxygen species and inhibiting the activation of p53 and MAPKs mediated stress response pathways.” Toxicology Reports 2015; 2: 365–76.
• Yousef MI, Saad AA, El-Shennawy LK.  Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food Chem Toxicol  2009; 47 : 1176-83.
• Alhaider AA, Korashy HM, Sayed-Ahmed MM, Mobark M, Kfoury H, Mansour MA. Metformin attenuates streptozotocin- induced diabetic nephropathy in rats through modulation of oxidative stress genes expression. Chemico-Biological Interactions 2011; 192: 233-42.
• Chirina YI, Jose PC. Sisplatin kaynaklı nefrotoksisitede oksidatif ve nitrozatif stresin rolü. Deneysel ve Toksikolojik Patoloji 2009; 61: 223–42.
• Noyan A. Fizyoloji.8.baskı. Meteksan A.Ş. Ankara; 1993.
• Schwartz PJ, Stramba-Badiale M, Segantini A, et al. Prolongation of the QT interval and the sudden infant death syndrome. New Engl J Med 1998; 11:1709-14.
• Antselevitch C, Shimizu W. Cellular mechanisms underlying the long QT syndrome. Curr Opin Cardiol 2002; 17: 43-51.
• Akıta M., Kuwahara M., Tsubone H., Sugano S. ECG changes during furosemide-induced hypokalemia in The rat. J Electrocardiol 1998; 31: 45-9.
• Mackıewıcz U, Gerges JY, Chu S, et al. Ivabradine protects against ventricular arrhythmias in acute myocardial infarction in the rat. J Cell Physiol 2014; 229: 813-23.
• Hamdy Dalia A MSc; Brocks, Dion R PhD. Experimental hyperlipidemia causes an increase in the electrocardiographic changes associated with amiodarone. J Cardiovasc Pharmacol 2009; 53: 1-8.
• Takahara A, Sugiyama A, Hashimoto K.  Reduction of repolarization reserve by halothane anaesthesia sensitizes the guinea-pig heart for drug-induced QT interval prolongation. Br J Pharmacol 2005;146: 561–7.
• Hashemzaei M, Barani AK, Iranshahi M, et al. Effects of resveratrol on carbon monoxide-induced cardiotoxicity in rats. Environ Toxicol Pharmacol 2016; 46:110-5.
• Demrow HS, Slane PR, Folts JD. Administrator of wine and Grape juice inhibits in vivo platelet activity and trombosis in sterosed canine coronary arteries. Circulation 1995; 91:82-8.
• Chen CK, Pace-Asciak CR. Vasorelaxing Activity of Resveratrol and Quercetin in Isolated Rat Aorta. Gen Pharmacol 1996; 27: 363-6.
• Wollny T, Aiello L, Tommaso DD, et al. Modulation of haemostatic function and prevention of experimental trombosis by red wine in rats: a role for increased nitric oxide production. B J Pharmacol 1999; 127: 747-55.
• Stef G, Csiszar A, Lerea K, Ungvari Z, Veress G. Resveratrol inhibits aggregation of platelets from high-risk cardiac patients with aspirin resistance. J Cardiovasc Pharmacol 2006; 48: 1-5.
• Buschmann G, Schumacher W, Budden R, Kühl UG. Evaluation of the effect of dopamine and othercatecholamines on the electrocardiogram and blood pressure of rats by means of on-line biosignal processing. J Cardiovasc Pharmacol 1980; 2: 777-95.
• Ahmad A, Sattar MZ, Rathore HA, et al. Impact of Isoprenaline and Caffeine on development of left ventricular hypertrophy and renal hemodynamic in Wistar Kyoto rats. Acta Pol Pharm 2015;72: 1015-26.
• Vilar-Pereira G, Carneiro VC, Mata-Santos H, et al. Resveratrol reverses functional chagas heart disease in mice. PLoS pathogens 2016; 12: e1005947.
• Kuzmin AI, Gourine AV, Molosh AI, Lakomkin VL, Vassort G. Effects of preconditioning on myocardial interstitial levels of ATP and its catabolites during regional ischemia and reperfusion in the rat. Basic Res Cardiol 2000; 95:127-36.