Impact of application of alumina oxide nanoparticles on callus induction, pigment content, cell damage and antioxidant enzyme activities in Ocimum basilicum

Abstract

With the advancement of nanotechnology, various potential applications of nanoparticles (NPs) have attracted considerable attention. In recent years, plant tissue culture applications in agricultural nanotechnology have become more popular. However, there are very few studies evaluating the effect of aluminum oxide nanoparticles (Al2O3 NPs) on enzyme activity and pigment content after plant tissue culture application. For this purpose, Ocimum basilicum callus growth effects, lipid peroxidation, hydrogen peroxide (H2O2) scavenging activity and chlorophyll content were investigated. As a result of this application, callus formation percentage and callus weight of the stem segment were found to be better than the leaf as the source of explants. The highest callus formation percentage (100%) was recorded as 741 mg stem and 324 mg leaf in (MS + 75 mg/l Al2O3) nutrient medium. In the (B5 + 75 mg/l Al2O3) nutrient medium, the stem was found to be 675 mg and the leaf 350 mg. Stress caused by Al2O3NP application was evaluated by chlorophyll and carotenoid pigment measurement.The highest Chl-a was detected at 75 mg / l Al2O3 NP concentration. The lowest total carotenoid was reported at 100 mg/l. The lowest Chl-a was detected at 25 mg / l. It was observed that the test groups treated with Al2O3 nanoparticle were significantly higher than the control group. In particular, the malondialdehit (MDA) level at 50 mg/l was quite high (7,409 times compared to control).

Keywords

• Al2O3
• nanoparticles
• plant tissue culture
• MDA
• chlorophyll
• pigment

Supporting Institution

ordu üniversitesi bilimsel araştırma projeleri birimi

Project Number

AR-1639

Thanks

The authors are grateful to the Coordination Unit of Scientific Research Projects of Ordu University (ODU - BAP) for providing financial support for this work under Project No. AR-1639.

References

1. Açıkgöz MA, (2020) Establishment of cell suspension cultures of Ocimum basilicum L. and enhanced production of pharmaceutical active ingredients. Industrial Crops and Products, 148, 112278. http://dx.doi.org/10.1016/j.indcrop.2020.112278.
2. Açıkgöz MA, Kara ŞM, Aygün A, Özcan MM, Ay EB, (2019) Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow (Achillea gypsicola) species. Turkish Journal of Agriculture and Forestry, 43(3), 351-359. http://dx.doi.org/10.3906/tar-1809-54.
3. Açıkgöz MA, (2021) Effects of sorbitol on the production of phenolic compounds and terpenoids in the cell suspension cultures of Ocimum basilicum L. Biologia 76, 395–409. https://doi.org/10.2478/s11756-020-00581-0.
4. Barrena R, Casals E, Colón J, Font X, Sánchez A, Puntes V, (2009) Evaluation of the ecotoxicity of model nanoparticles. Chemosphere, 75(7), 850-857.
5. Bilal A, Jahan N, Ahmed A, Bilal SN, Habib S, Hajra S, (2012) Phytochemical and pharmacological studies onOcimum basilicumLinn–a review. Int J Curr Res Rev 4.
6. Biswal B, Biswal UC, (1999) Photosynthesis under stress: signals and adaptive response of chloroplasts. In: Pessarakli, M. (Ed.), Handbook of Plant and Crop Stress. Marcel Dekker Inc, New York, pp. 315–336.
7. Curran PJ, Dungan JL, Gholz HL, (1990) Exploring the relationship between reflectance red edge and chlorophyll content in slash pine. Tree Physiol 7: 33 –48.
8. Dağlıoğlu Y, Yilmaz-Ozturk B, (2018a) A comparison of the acute toxicity and bioaccumulation of boron particles (nano and micro) in chodatodesmus mucronulatus. Bor Dergisi, 3(3), 157-165.

9. Dağlıoğlu Y, Yılmaz Öztürk B, (2018b) Effect of concentration and exposure time of ZnO-TiO2 nanocomposite on photosynthetic pigment contents, ROS production ability, and bioaccumulation of freshwater algae (Desmodesmus multivariabilis). Caryologia, 71(1), 13-23.
10. Dağlıoğlu Y, Yılmaz Öztürk B, (2016) The assessment of biological accumulation on exposure in boron particles of Desmodesmus multivariabilis. Biological Diversity and Conservation, 9(3), 204-209.
11. Özturk BY, Daglioglu Y, (2021) Extracellular synthesis of silver nanoparticles using Cladophora sp. and its antimicrobial activity effects on lipid peroxidation and antioxidant activites of extracts. Fresenius Environmental Bulletin, Volume 30– No. 06B/2021 pages 7422-7432.
12. Dhas BC, Simon NS, Austin P, Selvan S, Rajaram SK, Alexander RA, (2016) Comparative effects of plant growth regulators on callus induction in leaf explants of Wedelia chinensis. South Indian Journal of Biological Sciences, 2(2): 320-325.
13. Filella I, Serrano I, Serra J, Peñuelas J, (1995) Evaluating wheat nitrogen status with canopy relfectance indices and discriminant analysis. Crop Sci 35: 1400–1405.
14. Godhwani S, Godhwani JL, Vyas DS, (1998) Ocimum sanctum – a preliminary study evaluating its immunoregulatory profile in albino rats. J Ethnopharmacol 24: 193–198.
15. Gülçin I, Elmastaş M, Aboul‐Enein HY, (2007) Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytotherapy research, 21(4), 354-361.
16. Gülçin I, Oktay M, Kireçci E, Küfrevioglu ÖI, (2003) Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem 83: 371–382.
17. Handy RD, von der Kammer F, Lead JR, Hassellov M, Owen R, Crane M, (2008) The ecotoxicology and chemistry of manufactured nanoparticles. Ecotoxicology17, 287–314.
18. Hariprasath L, Jegadeesh R, Arjun P, Raaman N, (2015) In vitro propagation of Senecio candicans DC and comparative antioxidant properties of aqueous extracts of the in vivo plant and in vitro-derived callus. South African Journal of Botany, 98: 134-141.
19. Hosseini Z, Ghasempour HR, Kahrizi D, Akbari L, (2017) In vitro Callus Induction and Shoot Regeneration in Hollyhocks (Althaea digitata). Biological, Environmental and Agricultural Sciences, 2(1): 34-40.
20. Hussain AI, Anwar F, Sherazi STH, Przybylski R, (2008) Chemical com-position, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem108:986–995.
21. Jin L, Yang Y, Gao W, Gong M, Wang J, Anderson NO, He M, (2017) Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema indicum var. Aromaticum a Scented Chrysanthemum. Journal of Plant Studies, 6(2): 38.
22. Johnson N, Sanders P, (2012) High strength low alloy (Hsla) aluminum. Interna-tional Journal of Metalcasting 6, 61–62.
23. Khanpour-Ardestani N, Sharifi M, Behmanesh M, (2015) Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production. Cytotechnology, 67(3): 475-485.
24. Khot, L R, Sankaran S, Maja JM, Ehsani R, Schuster EW, (2012) Applications of nanomaterials in agricultural production and crop protection: a review. Crop protection, 35, 64-70.
25. Kim YJ, Choi HS, Song MK, Youk DY, Kim JH, Ryu JC, (2009) Genotoxicityof aluminum oxide (Al1O3) nanoparticle in mammalian cell lines. Molecular &Cellular Toxicology 5, 172–178.
26. Krishnan SS, Siril EA, (2017) Enhanced in vitro shoot regeneration in Oldenlandia umbellata L. by using quercetin: A naturally occurring auxin-transport Inhibitor. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 87(3): 899-904.
27. Kumari S, Pandey RK, Kumar U, (2015) In vitro callus induction from two different explants stem and leaf in Carthamus tinctorius Linn. European Journal of Experimental Biology, 5(2): 1-4.
28. Lee DH, Kim, YS, Lee CB, (2001) The inductive responses of the antioxidant enzymes by salt stress in rice (Oryza sativa L.). Journal of Plant Physiology, 158, 737–745.
29. Limma-Netto JD, Oliveira RSM, Copatti CE, (2017) Efficiency of essen-tial oils ofOcimum basilicumandCymbopogum flexuosusin thesedation and anaesthesia of NileTilapia juveniles.AnAcadBrasCiênc 89:2971–2974 .
30. Liu DH, Zou, J, Meng QM, Zou, JH., Jiang, WS, (2009) Uptake and accumulation and oxidative stress in garlic (Allium sativum L.) under lead phytotoxicity. Ecotoxicology, 18, 134–143.
31. Lukin ES, Tarasova SV, Korolev AV, (2001) Application of ceramics based onaluminum oxide in medicine (a review). Glass and Ceramics 58, 105–107.
32. Lutts S, Kinet JM, Bouharmont J, (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. Plant Growth Reg. 19, 207–218.
33. Makri O, Kintzios S, (2007) Ocimum sp. (basil): botany, cultivation, phar-maceutical properties, and biotechnology. Int J Geogr Inf Syst 13:123–150.
34. Marwat SK, Khan MS, Ghulam S, Anwar N, Mustafa G, Usman K, (2011) Phytochemical constituents and pharmacological activitiesof sweet basil–Ocimum basilicumL. (Lamiaceae). Asian J Chem9:3773–3782 .
35. Mata AT, Proença C, Ferreira AR, Serralheiro MLM, Nogueira JMF, Araújo MEM, (2007) Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food chemistry, 103(3), 778-786.
36. Maynard AD, Aitken RJ, (2007) Assessing exposure to airborne nanomaterials: current abilities and future requirements. Nanotoxicology, 1(1), 26-41.
37. Merzlyak MN, Gitelson AA, (1995) Why and what for the leaves are yellow in autumn? On the interpretation of optical spectra of senescing leaves (Acer platanoides L.). J Plant Physiol 145: 315 –320.
38. Merzlyak MN, Gitelson AA, Chivkunova OB, Rakitin VY, (1999) Nondestructive optical detection of leaf senescence and fruit ripening. Physiol Plant 106: 135 –141.
39. Moran, J. A., Mitchell, A. K., Goodmanson, G., Stockburger, KA 2000 Differentiation among effects of nitrogen fertilization treatments on conifer seedlings by foliar reflectance: a comparison of methods. Tree Physiol 20: 1113–1120.
40. Nel A, Xia T, Mädler L, Li, N 2006 Toxic potential of materials at the nanolevel. Science, 311(5761), 622-627.
41. Odabaş F, (1981) Bacchus Çeşidinde (Vitis Vinifera L.) Yaprakların klorofil miktarı üzerine azot gübrelemesinin etkisi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 12(2): 39-50.
42. Ohkawa H, Ohishi N, Yagi K, (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 95(2), 351-358.
43. Piras A, Gonçalves MJ, Alves J, Falconieri D, Porcedda S, Maxia A, Salgueiro L, (2018) Ocimum tenuiflorum L. and Ocimum basilicum L., two spices of Lamiaceae family with bioactive essential oils. IndCrop Prod 113:89–97.
44. Prashar R, Kumar A, Banerjee S, Rao AR, (1994) Chemopreventive action by an extract from Ocimum sanctum on mouse skin papilloma genesis and its enhancement of skin glutathione s-transferase activity and acid soluble sulfydryl level. Anticancer Drugs 5: 567–572.
45. Radotic K, Ducic T, Mutavdzic D, (2000) Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium. Environ. Exp. Bot., 44, 105–13.
46. Rico CM, Majumdar S, Duarte-Gardea M, Peralta-Videa JR, Gardea-Torresdey JL, (2011) Interaction of nanoparticles with edible plants and their possible implications in the food chain. Journal of agricultural and food chemistry, 59(8), 3485-3498.
47. Ruan SY, Schuh CA, (2012) Towards electroformed nanostructured aluminumalloys with high strength and ductility. Journal of Materials Research 27, 1638–1651
48. Ruch KJ, Cheng SJ, Klaunig JE, (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechinisolated from Chinese green tea. Carcinogensis. 10,1003-1008.
49. Sevengör S, Yasar F, Kusvuran S, Ellialtioglu S, (2011) The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. African Journal of Agricultural Research, 6(21), 4920-4924.
50. Siddiqui BS, Bhatti HA, Begum S, Perwaiz S, (2012) Evaluation of theantimycobacterium activity of the constituents fromOcimumbasilicumagainstMycobacterium tuberculosis. J Ethnopharmacol144:220–222.
51. Snoussi M, Dehmani A, Noumi E, Flamini G, Papetti A, (2016) Chemicalcomposition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibriospp. strains. MicrobPathog 90:13–21.
52. Yilmaz Öztürk B, Dağlıoğlu Y, Aşıkkutlu B, Akköz, C, (2018) Changes in pigment content of green algae (Desmodesmus sp. and Chodatodesmus mucranulatus) exposed to alumina oxide (Al2O3) nanoparticles. Biological Diversity and Conservation, 11(3), 64-70.
53. Yilmaz-Ozturk B, Daglioglu Y, (2018) The Ecotoxicological Effects Of ZnO-TiO2 Nanocomposite In Chodatodesmus mucranulatus. Feb-Fresenius Environmental Bulletin, 2951-2962.
54. Zinhari Z, Pourseyedi S, Zolala J, (2016) Callus induction and direct shoot regeneration in Lepidium draba L. explants. Journal of Agrıcultural Biotechnology, 8(2): 31-51.