Advanced electrospun biomaterials for skin tissue engineering

Gülşah Sunal; Cem Gök; Ümit Hüseyin Kaynar

doi:10.61112/jiens.1756938

Advanced electrospun biomaterials for skin tissue engineering

Abstract

Autografts, allografts or xenografts can be applied for treating damaged skin tissue, but these methods have certain disadvantages such as additional damage to donor regions, increased infection risk, and risk of disease transmission. On the other hand, tissue engineered skin substitutes provide more advantageous properties including large sources and good bioactivity. Tissue engineering is an interdisciplinary field of science that aims to develop tissue-engineered substitutes or tissue scaffolds in order to replace, repair, maintain, and regenerate the tissue functions. For producing functional tissue scaffolds for use in tissue engineering, different fabrication methods have been used by scientists. One such technique is electrospinning, which has been recognized as a promising method for creating microstructures that closely resemble the extracellular matrix of skin tissue. In this review article, the aim was first to provide information about skin tissue-related problems, current treatment methods, electrospinning method and its working principle, and to review the recent literature on the applications of electrospinning for use in skin tissue engineering.

Keywords

Thanks

This work was also supported by Izmir Bakırçay University Scientific Research Projects Coordination Unit, under grant number BBAP.2024.010.

References

Wei C, Feng Y, Che D, Zhang J, Zhou X, Shi Y, Wang L (2022) Biomaterials in skin tissue engineering. Int J Polym Mater Polym Biomater 71:993-1011. https://doi.org/10.1080/00914037.2021.1933977
Rahmati M, Mills DK, Urbanska AM, Saeb MR, Venugopal JR, Ramakrishna S, Mozafari M (2021) Electrospinning for tissue engineering applications. Prog Mater Sci 117:100721. https://doi.org/10.1016/j.pmatsci.2020.100721
Ramakrishna S, Mayer J, Wintermantel E, Leong KW (2001) Biomedical applications of polymer-composite materials: a review. Compos Sci Technol 61:1189-1224. https://doi.org/10.1016/S0266-3538(00)00241-4
Zhang LG, Khademhosseini A, Webster T (Eds.) (2016). Tissue and Organ Regeneration: Advances in Micro-and Nanotechnology. CRC Press
Kong CS, Yoo WS, Jo NG, Kim HS (2010) Electrospinning mechanism for producing nanoscale polymer fibers. J Macromol Sci Part B Phys 49:122-131. https://doi.org/10.1080/00222340903344390
Lannutti J, Reneker D, Ma T, Tomasko D, Farson D (2007) Electrospinning for tissue engineering scaffolds. Mater Sci Eng, C 27:504-509. https://doi.org/10.1016/j.msec.2006.05.019
Bender DA (2009) A dictionary of food and nutrition. OUP Oxford
Venus M, Waterman J, McNab I (2010) Basic physiology of the skin. Surgery (Oxf) 28:469-472. https://doi.org/10.1016/j.mpsur.2010.07.011

Nyame TT, Chiang HA, Leavitt T, Ozambela M, Orgill DP (2015) Tissue-engineered skin substitutes. Plast Reconst Surg 136:1379-1388. https://doi.org/10.1097/PRS.0000000000001748
Böttcher-Haberzeth S, Biedermann T, Reichmann E (2010) Tissue engineering of skin. Burns 36:450-460. https://doi.org/10.1016/j.burns.2009.08.016
Kanitakis J (2002) Anatomy, histology and immunohistochemistry of normal human skin. Eur J Dermatol 12:390-401.
Ramanathan G, Singaravelu S, Raja MD, Nagiah N, Padmapriya P, Ruban K, ... & Perumal PT (2016) Fabrication and characterization of a collagen coated electrospun poly (3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications. RSC Adv 6:7914-7922. https://doi.org/10.1039/C5RA19529B
Chaudhari AA, Vig K, Baganizi DR, Sahu R, Dixit S, Dennis V, ... & Pillai SR (2016) Future prospects for scaffolding methods and biomaterials in skin tissue engineering: a review. Int J Mol Sci 17:1974. https://doi.org/10.3390/ijms17121974
Keirouz A, Chung M, Kwon J, Fortunato G, Radacsi N (2020) 2D and 3D electrospinning technologies for the fabrication of nanofibrous scaffolds for skin tissue engineering: A review. Wiley Interdiscip Rev Nanomed Nanobiotechnol 12:e1626. https://doi.org/10.1002/wnan.1626
Sundaramurthi D, Vasanthan KS, Kuppan P, Krishnan UM, Sethuraman S (2012) Electrospun nanostructured chitosan–poly (vinyl alcohol) scaffolds: a biomimetic extracellular matrix as dermal substitute. Biomed Mater 7:045005. https://doi.org/10.1088/1748-6041/7/4/045005
Olczyk P, Mencner Ł, Komosinska-Vassev K (2014) The role of the extracellular matrix components in cutaneous wound healing. Biomed Res Int 2014:747584. https://doi.org/10.1155/2014/747584
Gantwerker EA, Hom DB (2012) Skin: histology and physiology of wound healing. Clin Plast Surg 39:85-97. https://doi.org/10.1016/j.cps.2011.09.005
Rahmati M, Blaker JJ, Lyngstadaas SP, Mano JF, Haugen HJ (2020) Designing multigradient biomaterials for skin regeneration. Mater Today Adv 5:100051. https://doi.org/10.1016/j.mtadv.2019.100051
Schultz GS, Sibbald RG, Falanga V, Ayello EA, Dowsett C, Harding K, ... & Vanscheidt W (2003) Wound bed preparation: a systematic approach to wound management. Wound Repair Regener 11:S1-S28. https://doi.org/10.1046/j.1524-475X.11.s2.1.x
Fletcher J (2008) Differences between acute and chronic wounds and the role of wound bed preparation. Nursing Standard (through 2013), 22:62.
Goonoo N, Bhaw-Luximon A (2020) Nanomaterials combination for wound healing and skin regeneration. In: Advanced 3D-printed systems and nanosystems for drug delivery and tissue engineering. Elsevier, pp 159-217.
WHO (2019) Burns. World Health Organization (WHO). https://www.who.int/news-room/fact-sheets/detail/burns. Accessed 13 October 2023
Ding J, Zhang J, Li J, Li D, Xiao C, Xiao H, ... & Chen X (2019) Electrospun polymer biomaterials. Prog Polym Sci 90:1-34. https://doi.org/10.1016/j.progpolymsci.2019.01.002
MacNeil S (2007) Progress and opportunities for tissue-engineered skin. Nature 445:874-880. https://doi.org/10.1038/nature05664
Valencia IC, Falabella AF, Eaglstein WH (2000) Skin grafting. Dermatol Clin 18:521-532. https://doi.org/10.1016/S0733-8635(05)70199-6
Phillips TJ (1988) Cultured skin grafts: past, present, future. Arch Dermatol 124:1035-1038. https://doi.org/10.1001/archderm.1988.01670070023012
Dreifke MB, Jayasuriya AA, Jayasuriya AC (2015) Current wound healing procedures and potential care. Mater Sci Eng, C 48:651-662. https://doi.org/10.1016/j.msec.2014.12.068
Alaribe FN, Manoto SL, Motaung SC (2016) Scaffolds from biomaterials: advantages and limitations in bone and tissue engineering. Biologia 71:353-366. https://doi.org/10.1515/biolog-2016-0056
Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS (2011) Polymeric scaffolds in tissue engineering application: a review. Int J Polym Sci 2011:290602. https://doi.org/10.1155/2011/290602
Owida HA, Al-Nabulsi JI, Alnaimat F, Al-Ayyad M, Turab NM, Al Sharah A, Shakur M (2022) Recent applications of electrospun nanofibrous scaffold in tissue engineering. Appl Bionics Biomech 2022:1953861. https://doi.org/10.1155/2022/1953861
Boyce ST (2001) Design principles for composition and performance of cultured skin substitutes. Burns 27:523-533. https://doi.org/10.1016/S0305-4179(01)00019-5
Kamel RA, Ong JF, Eriksson E, Junker JP, Caterson EJ (2013) Tissue engineering of skin. J Am Coll Surg 217:533-555. https://doi.org/10.1016/j.jamcollsurg.2013.03.027
Zhong SP, Zhang YZ, Lim CT (2010) Tissue scaffolds for skin wound healing and dermal reconstruction. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:510-525. https://doi.org/10.1002/wnan.100
MacNeil S (2008) Biomaterials for tissue engineering of skin. Mater Today 11:26-35. https://doi.org/10.1016/S1369-7021(08)70087-7
Kumbar SG, Nair LS, Bhattacharyya S, Laurencin CT (2006) Polymeric nanofibers as novel carriers for the delivery of therapeutic molecules. J Nanosci Nanotechnol 6:2591-2607. https://doi.org/10.1166/jnn.2006.462
Wu C, Chen T, Xin Y, Zhang Z, Ren Z, Lei J, ... & Tang S (2016) Nanofibrous asymmetric membranes self-organized from chemically heterogeneous electrospun mats for skin tissue engineering. Biomed Mater 11:035019. https://doi.org/10.1088/1748-6041/11/3/035019
Han F, Dong Y, Su Z, Yin R, Song A, Li S (2014) Preparation, characteristics and assessment of a novel gelatin–chitosan sponge scaffold as skin tissue engineering material. Int J Pharm 476:124-133. https://doi.org/10.1016/j.ijpharm.2014.09.036
Santangelo S (2019) Electrospun nanomaterials for energy applications: Recent advances. Appl Sci 9:1049. https://doi.org/10.3390/app9061049
Wang C, Wang J, Zeng L, Qiao Z, Liu X, Liu H, ... & Ding J (2019) Fabrication of electrospun polymer nanofibers with diverse morphologies. Molecules, 24:834. https://doi.org/10.3390/molecules24050834
Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S (2003) A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 63:2223-2253. https://doi.org/10.1016/S0266-3538(03)00178-7
Andrady AL, Ensor DS, Walker TA, Prabhu P (2009) Nanofiber mats and production methods thereof. U.S. Patent No. 7, 592, 277. Washington, DC: U.S. Patent and Trademark Office, Google Patents.
Doshi J, Reneker DH (1995) Electrospinning process and applications of electrospun fibers. J Electrostat 35:151-160. https://doi.org/10.1016/0304-3886(95)00041-8
Srinivasan G, Reneker DH (1995) Structure and morphology of small diameter electrospun aramid fibers. Polym Int 36:195-201. https://doi.org/10.1002/pi.1995.210360210
Reneker DH, Chun I (1996) Nanometre diameter fibres of polymer, produced by electrospinning. Nanotechnol 7:216. https://doi.org/10.1088/0957-4484/7/3/009
Kong CS, Lee TH, Lee SH, Kim HS (2007) Nano-web formation by the electrospinning at various electric fields. J Mater Sci 42:8106-8112. https://doi.org/10.1007/s10853-007-1734-3
Liu W, Thomopoulos S, Xia Y (2012) Electrospun nanofibers for regenerative medicine. Adv Healthcare Mater 1:10-25. https://doi.org/10.1002/adhm.201100021
Garg K, Bowlin GL (2011) Electrospinning jets and nanofibrous structures. Biomicrofluidics 5. https://doi.org/10.1063/1.3567097
Ding Y, Xu W, Xu T, Zhu Z, Fong H (2019) Theories and principles behind electrospinning. In: Advanced Nanofibrous Materials Manufacture Technology Based on Electrospinning. CRC Press, pp 22-51.
Liu Y, Wang C (Eds.) (2019) Advanced nanofibrous materials manufacture technology based on electrospinning. CRC Press.
Wang Z, Crandall C, Sahadevan R, Menkhaus TJ, Fong H (2017) Microfiltration performance of electrospun nanofiber membranes with varied fiber diameters and different membrane porosities and thicknesses. Polymer 114:64-72. https://doi.org/10.1016/j.polymer.2017.02.084
Lin YP, Lin SY, Lee YC, Chen-Yang YW (2013) High surface area electrospun prickle-like hierarchical anatase TiO 2 nanofibers for dye-sensitized solar cell photoanodes. J Mater Chem A 1:9875-9884. https://doi.org/10.1039/C3TA10925A
Sabba D, Mathews N, Chua J, Pramana SS, Mulmudi HK, Wang Q, Mhaisalkar SG (2013) High-surface-area, interconnected, nanofibrillar TiO2 structures as photoanodes in dye-sensitized solar cells. Scr Mater 68:487-490. https://doi.org/10.1016/j.scriptamat.2012.11.027
Yoshimoto H, Shin YM, Terai H, Vacanti JP (2003) A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomater 24:2077-2082. https://doi.org/10.1016/S0142-9612(02)00635-X
Smith LA, Ma PX (2004) Nano-fibrous scaffolds for tissue engineering. Colloids Surf, B 39:125-131. https://doi.org/10.1016/j.colsurfb.2003.12.004
Bacakova M, Musilkova J, Riedel T, Stranska D, Brynda E, Zaloudkova M, Bacakova L (2016) The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering. Int J Nanomed 11:771-789. https://doi.org/10.2147/IJN.S99317
Vig K, Chaudhari A, Tripathi S, Dixit S, Sahu R, Pillai S, ... & Singh SR (2017) Advances in skin regeneration using tissue engineering. Int J Mol Sci 18:789. https://doi.org/10.3390/ijms18040789
Sundaramurthi D, Krishnan UM, Sethuraman S (2014) Electrospun nanofibers as scaffolds for skin tissue engineering. Polym Rev 54:348-376.
Khil MS, Cha DI, Kim HY, Kim IS, Bhattarai N (2003) Electrospun nanofibrous polyurethane membrane as wound dressing. J Biomed Mater Res Part B: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and The Korean Society for Biomaterials 67:675-679. https://doi.org/10.1002/jbm.b.10058
Dong Y, Zheng Y, Zhang K, Yao Y, Wang L, Li X, ... & Ding B (2020) Electrospun nanofibrous materials for wound healing. Adv Fiber Mater 2:212-227. https://doi.org/10.1007/s42765-020-00034-y
Liu H, Wang C, Li C, Qin Y, Wang Z, Yang F, ... & Wang J (2018) A functional chitosan-based hydrogel as a wound dressing and drug delivery system in the treatment of wound healing. RSC Adv 8:7533-7549. https://doi.org/10.1039/C7RA13510F
Sun J, Perry SL, Schiffman JD (2019) Electrospinning nanofibers from chitosan/hyaluronic acid complex coacervates. Biomacromolecules 20:4191-4198. https://doi.org/10.1021/acs.biomac.9b01072
Singh R, Khan S, Basu SM, Chauhan M, Sarviya N, Giri J (2019) Fabrication, characterization, and biological evaluation of airbrushed gelatin nanofibers. ACS Appl Bio Mater 2:5340-5348. https://doi.org/10.1021/acsabm.9b00636
Dodero A, Scarfi S, Pozzolini M, Vicini S, Alloisio M, Castellano M (2019) Alginate-based electrospun membranes containing ZnO nanoparticles as potential wound healing patches: biological, mechanical, and physicochemical characterization. ACS Appl Mater Interfaces 12:3371-3381. https://doi.org/10.1021/acsami.9b17597
Simões D, Miguel SP, Ribeiro MP, Coutinho P, Mendonça AG, Correia IJ (2018) Recent advances on antimicrobial wound dressing: A review. Eur J Pharm Biopharm 127:130-141. https://doi.org/10.1016/j.ejpb.2018.02.022
Liu Y, Li T, Han Y, Li F, Liu Y (2021) Recent development of electrospun wound dressing. Curr Opin Biomed Eng 17:100247. https://doi.org/10.1016/j.cobme.2020.100247
Evrova O, Bürgisser GM, Ebnöther C, Adathala A, Calcagni M, Bachmann E, ... & Buschmann J (2020) Elastic and surgeon friendly electrospun tubes delivering PDGF-BB positively impact tendon rupture healing in a rabbit Achilles tendon model. Biomaterials, 232:119722. https://doi.org/10.1016/j.biomaterials.2019.119722
Li Y, Xiao Z, Zhou Y, Zheng S, An Y, Huang W, ... & Wu J (2019) Controlling the multiscale network structure of fibers to stimulate wound matrix rebuilding by fibroblast differentiation. ACS Appl Mater Interfaces 11:28377-28386. https://doi.org/10.1021/acsami.9b06439
Memic A, Abdullah T, Mohammed HS, Joshi Navare K, Colombani T, Bencherif SA (2019) Latest progress in electrospun nanofibers for wound healing applications. ACS Appl Bio Mater 2:952-969. https://doi.org/10.1021/acsabm.8b00637
Pires F, Santos JF, Bitoque D, Silva GA, Marletta A, Nunes VA, ... & Raposo M (2019) Polycaprolactone/gelatin nanofiber membranes containing EGCG-loaded liposomes and their potential use for skin regeneration. ACS Appl Bio Mater 2:4790-4800. https://doi.org/10.1021/acsabm.9b00524
Cesur S, Oktar FN, Ekren N, Kilic O, Alkaya DB, Seyhan SA, ... & Gunduz O (2020) Preparation and characterization of electrospun polylactic acid/sodium alginate/orange oyster shell composite nanofiber for biomedical application. J Aust Ceram Soc 56:533-543. https://doi.org/10.1007/s41779-019-00363-1
Nagam Hanumantharao S, Rao S (2019) Multi-functional electrospun nanofibers from polymer blends for scaffold tissue engineering. Fibers 7:66. https://doi.org/10.3390/fib7070066
Abid S, Wang L, Haider MK, Mayakrishnan G, Lakshminarayanan R, Kim KO, ... & Kim IS (2024) Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study. Nanocomposites 10:242-255. https://doi.org/10.1080/20550324.2024.2362534
Movahedi M, Asefnejad A, Rafienia M, Khorasani MT (2020) Potential of novel electrospun core-shell structured polyurethane/starch (hyaluronic acid) nanofibers for skin tissue engineering: In vitro and in vivo evaluation. Int J Biol Macromol 146:627-637. https://doi.org/10.1016/j.ijbiomac.2019.11.233
Arampatzis AS, Kontogiannopoulos KN, Theodoridis K, Aggelidou E, Rat A, Willems A, ... & Assimopoulou AN (2021) Electrospun wound dressings containing bioactive natural products: Physico-chemical characterization and biological assessment. Biomater Res 25:23. https://doi.org/10.1186/s40824-021-00223-9
Massoumi B, Sarvari R, Fakhri E (2024) Conductive electrospun nanofiber based on silk fibroin/cellulose nanocrystals/reduced graphene oxide as a wound healing material. Int J Polym Mater Polym Biomater 73:1197-1206. https://doi.org/10.1080/00914037.2023.2255722
Wang S, Xu X, Zhu X, Tan X, Xie B (2024) Electrospun Carvacrol-Loaded Polyacrylonitrile/Poly (ethylene oxide) Nanofibrous Films as Wound Dressings. ACS Omega 9:39472-39483. https://doi.org/10.1021/acsomega.4c03140
Jadbabaei S, Kolahdoozan M, Naeimi F, Ebadi-Dehaghani H (2021) Preparation and characterization of sodium alginate–PVA polymeric scaffolds by electrospinning method for skin tissue engineering applications. RSC Adv 11:30674-30688. https://doi.org/10.1039/D1RA04176B
Lotfi Z, Khakbiz M, Davari N, Bonakdar S, Mohammadi J, Shokrgozar MA, Derhambakhsh S (2023) Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing. Artif Organs 47:1267-1284. https://doi.org/10.1111/aor.14518
Choi JI, Kim MS, Chung GY, Shin HS (2017) Spirulina extract-impregnated alginate-PCL nanofiber wound dressing for skin regeneration. Biotechnol Bioprocess Eng 22:679-685. https://doi.org/10.1007/s12257-017-0329-3
Phulmogare G, Rani S, Lodhi S, Patil UK, Sinha S, Gupta U (2024) Fucoidan loaded PVA/Dextran blend electrospun nanofibers for the effective wound healing. Int J Pharm 650:123722. https://doi.org/10.1016/j.ijpharm.2023.123722
Pajooh AMD, Tavakoli M, Al-Musawi MH, Karimi A, Salehi E, Nasiri-Harchegani S, ... & Mirhaj M (2024) Biomimetic VEGF-loaded bilayer scaffold fabricated by 3D printing and electrospinning techniques for skin regeneration. Mater Des 238:112714. https://doi.org/10.1016/j.matdes.2024.112714

Details

Primary Language

English

Subjects

Materials Engineering (Other)

Journal Section

Review Article

Authors

Gülşah Sunal ^*
0000-0001-7768-922X
Türkiye

Cem Gök
0000-0002-8949-8129
Türkiye

Ümit Hüseyin Kaynar
0000-0002-3321-0341
Türkiye

Publication Date

January 31, 2026

Submission Date

August 2, 2025

Acceptance Date

November 25, 2025

Published in Issue

Year 2026 Volume: 6 Number: 1

DOI

https://doi.org/10.61112/jiens.1756938

IZ

https://izlik.org/JA33RZ28PU

Cite

RIS / Bibtex

APA

Sunal, G., Gök, C., & Kaynar, Ü. H. (2026). Advanced electrospun biomaterials for skin tissue engineering. Journal of Innovative Engineering and Natural Science, 6(1), 133-145. https://doi.org/10.61112/jiens.1756938

AMA

1.Sunal G, Gök C, Kaynar ÜH. Advanced electrospun biomaterials for skin tissue engineering. JIENS. 2026;6(1):133-145. doi:10.61112/jiens.1756938

Chicago

Sunal, Gülşah, Cem Gök, and Ümit Hüseyin Kaynar. 2026. “Advanced Electrospun Biomaterials for Skin Tissue Engineering”. Journal of Innovative Engineering and Natural Science 6 (1): 133-45. https://doi.org/10.61112/jiens.1756938.

EndNote

Sunal G, Gök C, Kaynar ÜH (January 1, 2026) Advanced electrospun biomaterials for skin tissue engineering. Journal of Innovative Engineering and Natural Science 6 1 133–145.

IEEE

[1]G. Sunal, C. Gök, and Ü. H. Kaynar, “Advanced electrospun biomaterials for skin tissue engineering”, JIENS, vol. 6, no. 1, pp. 133–145, Jan. 2026, doi: 10.61112/jiens.1756938.

ISNAD

Sunal, Gülşah - Gök, Cem - Kaynar, Ümit Hüseyin. “Advanced Electrospun Biomaterials for Skin Tissue Engineering”. Journal of Innovative Engineering and Natural Science 6/1 (January 1, 2026): 133-145. https://doi.org/10.61112/jiens.1756938.

JAMA

1.Sunal G, Gök C, Kaynar ÜH. Advanced electrospun biomaterials for skin tissue engineering. JIENS. 2026;6:133–145.

MLA

Sunal, Gülşah, et al. “Advanced Electrospun Biomaterials for Skin Tissue Engineering”. Journal of Innovative Engineering and Natural Science, vol. 6, no. 1, Jan. 2026, pp. 133-45, doi:10.61112/jiens.1756938.

Vancouver

1.Gülşah Sunal, Cem Gök, Ümit Hüseyin Kaynar. Advanced electrospun biomaterials for skin tissue engineering. JIENS. 2026 Jan. 1;6(1):133-45. doi:10.61112/jiens.1756938