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Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province

Yıl 2020, , 2366 - 2377, 15.12.2020

Mustafa Usta , Abdullah Güller

https://doi.org/10.21597/jist.719099
Cited By: 3

Öz

Virus-like symptoms were observed in alfalfa plants (Medicago sativa L.) grown in Van province of Turkey in 2019. The symptoms observed were dwarfing, mosaic pattern and yellowing of leaves, rosetting, and decreasing in leaf sizes. Genomic RNA of 19 symptomatic and non-symptomatic alfalfa leaves were extracted for the detection of virus RNA using virus-specific primer pair by RT-PCR. Of the 19 alfalfa leaf specimens, the six yielded the expected 700 bp DNA band in severely symptomatic alfalfa specimens. Two of them randomly selected were inserted into a plasmid vector. Obtained recombinant plasmids were sequenced in both directions. According to the results of the sequence analysis, it was revealed that the virus in infected alfalfa was the Alfalfa mosaic virus. The sequence data were recorded into the GenBank with access numbers MT210179 and MT210178 and denominated as Alakoy Y9 and Alakoy Y1 isolates, respectively. According to the phylogenetic tree created with 16 AMV sequences registered in the GeneBank for both sequences, the AMV CP gene sequence indicated the highest similarity with USA (JN256023), Brazil (FJ858265), and Puglia (Y09110) isolate and the lowest with Korea isolate (KY348844), at the nucleotide level. It was also revealed that both sequences show 98.45% nucleotide similarity with 7 nucleotide exchanges. According to the literature research results, this is the first report and molecular analysis of Alfalfa mosaic virus (AMV) in Medicago sativa L. in Van province of Turkey.

Anahtar Kelimeler

Alfalfa RT-PCR characterisation sequencing phylogenetic analysis

Kaynakça

  • Abdalla OA, Al-Shahwan IM, AL-Saleh MA, Amer MA, 2019. Molecular characterization of Alfalfa mosaic virus (AMV) isolates in alfalfa and other plant species in different regions in Saudi Arabia. European Journal of Plant Pathology,156: 603–613.
  • Alan B, Crabtree K, Dallwitz M, Gibbs A, Watson L, 1996. Viruses of Plants. Description and Lists from the VIDE Database. CAB International, UK,1484 PP.
  • Alfaro-Fernández A, Sánchez-Navarro J A, Landeira M, Font MI, Hernández-Llópis D, Pallás V, 2016. Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and Tomato yellow leaf curl sardinia virus. Journal of Plant Pathology, 98 (2): 245-254.
  • Alhudaib KA, 2019. Serological and Molecular Detection of Alfalfa mosaic virus in the Major Potato Growing Areas of Saudi Arabia. Scientifc Journal of King Faisal University (Basic and Applied Sciences), 20 (1): 73- 82.
  • Ali H, Gholam K, 2011. Detection and Identification of Clavibacter michiganensis subsp. insidiosus inducing Alfalfa Wilt Disease Using Specific PCR and Physiological Methods in Iran. Research Journal of Chemistry and Environment, 15 (2): 486-491.
  • Al-Saleh MA, Amer MA, 2013. Biological and Molecular variability of Alfalfa mosaic virus affecting alfalfa crop in riyadh region. The Plant Pathology Journal, 29(4) : 410-417.
  • Al-Shahwan IM, Abdalla OA, Al-Saleh MA, Amer MA, 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
  • Anonim, 2014. T. C. Gıda Tarım ve Hayvancılık Bakanlığı, Tarla Bitkileri Araştırmaları Dairesi Başkanlığı, Çayır Mera ve Yem Bitkileri Araştırmaları Çalışma Grubu Raporu. https://www.tarimorman.gov.tr/TAGEM/Belgeler/SUNULAR/%C3%87MYB_Birg%C3%B Cl%20G%C3%BCner.PDF (Erişim Tarihi: 26.12.2018).
  • Anonymous 2017. Bugem Activities. T. C. Ministry of Food, Agriculture and Livestock, General Directorate of Plant Production, March 2017 (in Turkish).
  • Arlı-Sökmen M, Mennan H, Sevik MA, Ecevit O, 2005. Occurence of viruses in field-grown pepper crops and some of their reservoir weeds hosts in Samsun, Turkey. Phytoparasitica, 33(4), 347-358.
  • Bailiss KW, Ollennu LAA. 1986. Effect of Alfalfa mosaic virus isolates on forage yield of lucern (Medicago sativa) in Britain. Plant Pathology, 35: 162- 168.
  • Balasubramaniam M, Kim BS, Hutchens-Williams HM, Loesch-Fries LS, 2014. The photosystem II oxygen-evolving complex protein PsbP interacts with the coat protein of Alfalfa mosaic virus and inhibits virus replication. Molecular Plant-Microbe Interactions, 27(10): 1107- 1118.
  • Bol JF, 2003. Alfalfa mosaic virus: Coat protein dependent initiation of infection. Molecular Plant Pathology, 4(1): 1-8.
  • Brunt A, Crabtree K, Gibbs A, 1990. Viruses of Tropical Plants. CAB International, London.
  • Buzkan N, Demir M, Öztekin V, Mart C, Çağlar BK, Yilmaz MA, 2006. Evaluation of the status of capsicum viruses in the main growing regions of Turkey. Bulletin OEPP, 36(1): 15–19.
  • Chod J, Chodová D, Kočova M, Jokeš M, 1994. Occurrence of Alfalfa mosaic virus on red pepper (Capsicum annuum L.) and investigation of susceptibility of some cultivars with respect to Hill’s reaction activity and chlorophyll content. Zahradnictví, 21: 27–36.
  • Cook AA, Wilton AC, 1984. Alfalfa enation virus in the Kingdom of Saudi Arabia. FAO, Plant Protection Bulletin, 32: 139-140.
  • Çalı S, 1990. Orta Anadolu Bölgesi Yonca Ekim Alanlarında Görülen Virüs Enfeksiyonlarının Saptanması, Yabancı Otlar ve Tohumla Taşınması Üzerine Araştırmalar. Adana Zirai Mücadele Araştırma Ens. Müdürlüğü, Araştırma Yayınlan Serisi, Yayın No: 72.
  • Çetinkıran AD, Baloğlu S, 2011. Detection of the Alfalfa mosaic virus in pepper fields in Adana and Mersin. Ç.Ü Fen ve Mühendislik Bilimleri Dergisi, 26 (2): 89- 98.
  • Çulal Kılıç H, Yardımcı N, 2015. Occurrence of Alfalfa mosaic virus (AMV) infecting bean crop in Burdur Province, Turkey. Asian Journal of Agriculture and Food Sciences, 3(2): 173- 177.
  • Dale LM, Rotar I, Thewis A, Vidican R, Florian V, Ciure A, 2012. Determination of alfalfa crude fiber, ndf, adf and lignin content by nir spectrometry. Lucrări Ştiintifice, vol. 55, seria Agronomie, p: 245-249.
  • Demir M, 2005. Kahramanmaraş’ta Yetiştirilen Kırmızı Biberlerde Yaprakbiti İle Taşınan Virüslerin Saptanması. Kahramanmaraş Sütçü İmam Üniversitesi. Fen Bilimleri Enstitüsü. Bitki Koruma Anabilimdalı. Yüksek Lisans Tezi, Kahramanmaraş. 25s
  • El Helaly HS, Ahmed AA. Awad MA, Soliman AM, 2012. Biological and Molecular Characterization of Potato infecting Alfalfa mosaic virus in Egypt. International Journal of Virology, 8: 106- 113.
  • Erdiller G, Lesemann DE, 1985. Türkiye İçin Yeni Bir Virüs Hastalığı "Alfalfa Mosaic Virus". Ankara Üniv. Ziraat Fakültesi Yıllığı, 35: 20-25.
  • Fidan H, Adak NA, Konuksal A, Akerzurumlu E, Yılmaz MA, 2012. Occurrence of Alfalfa mosaic virus (AMV) diseases on potato crops in Northern Cyprus. Acta Horticulturae, 960: 341-346.
  • Foissac X, Svanella-Dumas L, Gentit P, Dulucq MJ, Candresse T, 2001. Polyvalent detection of fruit tree Tricho-,Capillo- and Foveaviruses by nested RT-PCR using degenerated and inosine-containing primers (PDO RT-PCR). Acta Horticulturae, 550: 37-44.
  • Forster RLS, Morris-Krsinich BAM, Musgrave DR, 1985. Incidence of Alfalfa mosaic virus, Lucerne Australian latent virus, and Lucerne transient streak virus in lucerne crops in the North Island of New Zealand New. New Zealand Journal of Agricultural Research, 28: 279-282.
  • Frencel I, Pospieszny H, 1979. Viruses in natural infections of yellow lupin (Lupinus luteus L.) in Poland. III. Alfalfa mosaic virus (AMV). Acta Phytopathologica Academiae Scientarum Hungaricae, 14(3–4): 269- 278.
  • Frosheiser FI, 1974. Alfalfa mosaic virus Transmission to Seed through Alfalfa Gametes and Longevity in Alfalfa Seed. Phytopathology, 64: 102 – 105.
  • Garran J, Gibbs A,. 1982. Studies on Alfalfa mosaic virus and alfalfa aphids. Australian Journal of Agricultural Research, 33: 657-664.
  • Geren H, Kir B, Demiroglu G, Kavut YT, 2009. Effects of different soil textures on the yield and chemical composition of alfalfa (Medicago sativa L.) cultivars under mediterranean climate conditions. Asian Journal of Chemistry, 21 (7) : 5517-5522.
  • Güzel Ö, Arlı-Sökmen M, 2003. Determination of some viruses infecting common bean (Phaseoulus vulgaris L.) and their incidences in seed lots in Samsun Province. The Journal of Turkish Phytopathology, 32 (2): 99-106.
  • Hajimorad MR, Francki RIB, 1988. Alfalfa mosaic virus isolates from lucerne in South Australia: Biological variability and antigenic similarity. The Annals of Applied Biology, 113: 45–54.
  • Hemmati K, Mclean DL, 1977. Gamete-seed Transmission of Alfalfa mosaic virus and its effect of Seed Germination and Yield in Alfalfa Plants. Phytopathology, 67: 576-579.
  • Hiruki C, Hampton RO, 1990. Alfalfa Mosaic. In: "Compendium of Alfalfa Diseases", (Eds.): Stuteville, D. L. and Erwin, D. C.. 2nd Edition, APS Press, St. Paul, 54 PP.
  • Jaspars EM, Bos L, 1980. Alfalfa mosaic virus. No. 229. In: Descriptions of plant viruses. Common wealth Mycology Institute/Association of Applied Biologists, Kew, England.
  • Kavut YT, Avcıoglu R, 2015. Yield and quality performances of various alfalfa (Medicago sativa L.) cultivars in different soil textures in a Mediterranean environment. Turkish Journal of Field Crops, 20(1): 65-71.
  • Mallor C, Luis-Arteaga M, Cambra MA, Fernandez-Cavada S, 2002. Disease Notes, 86: 698.
  • Mangeli F, Massumi H, Alipour F, Maddahian M, Heydarnejad J, Hosseinipour A, Amid-Motlagh MH, Azizizadeh M, Varsani A, 2019. Molecular and partial biological characterization of the coat protein sequences of Iranian Alfalfa mosaic virus isolates. Journal of Plant Pathology, 101: 73-742.
  • Martínez-Priego L, Córdoba MC, Jordá C, 2004. First Report of Alfalfa mosaic virus in Lavandula officinalis. Plant Disease, 88(8): 908.
  • Massumi H, Maddahian M, Heydarnejad J, Hosseini Pour A, Farahmand A, 2012. Incidence of Viruses Infecting Alfalfa in the Southeast and Central Regions of Iran. Journal of Agricultural Science and Technology, 14: 1141-1148.
  • McLaughlin MR, Boykin D, 1988. Virus Diseases of Seven Species of Forage Legumes in the Southeastern United States. Plant Disease, 72: 539-542.
  • Monteros MJ, Bouton JH, 2009.The future of alfalfa and forage crops In: Proceedings, Western Alfalfa & Forage Conference, December 2–4, Reno, Nevada.
  • Moreira AG, Kitajima EW, Rezende JA, 2010. Identification and partial characterization of a Carica papaya-infecting isolate of Alfalfa mosaic virus in Brazil. The Journal of General Plant Pathology, 76: 172- 175.
  • Moreira A, Fageria NK, 2010. Liming influence on soil chemical properties, nutritional status and yield of alfalfa grown in acid soil. The Revista Brasileira de Ciência do Solo (RBCS) , 34, p:1231-1239.
  • Mughal SM, Zadjali AD, Matrooshi AR, 2003. Occurrence, distribution and some properties of Alfalfa mosaic alfamovirus in the Sultanate of Oman. Pakistan Journal of Agricultural Sciences, 40(1-2): 67-73.
  • Nemchinov LG, Grinstead SC, Mollov DS, 2017. Alfalfa virus S, a new species in the family Alphaflexiviridae. Plos One, 12(5): 1-11.
  • Özdemir S, Erilmez, S. 2007. Denizli ilinde yetiştirilen biber, patlıcan ve marul üretim alanlarında bazı viral etmenlerin saptanması. Türkiye II. Bitki Koruma Kongresi Bildirileri.27-29 Ağustos Isparta. 114s.
  • Ozdemir S, Erilmez S, Paylan IC, 2011. First report of Alfalfa mosaic virus in eggplant in Turkey. The Journal of Plant Pathology (JPP), 93(4): 63-89.
  • Özdemir S, Erilmez S, Paylan Cİ, 2011. Ege Bölgesi Patates Üretim Alanlarında Alfalfa mosaic alfamovirus’ün Serolojik ve Moleküler Olarak Tanılanması. Türkiye IV. Bitki Koruma Kongresi Bildirileri: 410.
  • Paliwal YC, 1982. Virus diseases of alfalfa and biology of Alfalfa mosaic virus in Ontario and western Quebec. Canadian Journal of Plant Pathology, 4: 175- 179.
  • Palumbo JD, Phillips DA, Kado CI, 1998. Characterization of a new Agrobacterium tumefaciens strain from alfalfa (Medicago sativa L.). 1998. Archives of Microbiology, 169: 381–386.
  • Parrella G, Acanfora N, Orílio A, Navas-Castillo J, 2011. Complete nucleotide sequence of a Spanish isolate of Alfalfa mosaic virus: Evidence for additional genetic variability. Archives of Virology, 156, 1049–1052.
  • Parrella G, Cavicchi L, Bellardi MG, 2012. First Record of Alfalfa mosaic virus in Teucrium fruticans in Italy. Disease Notes. 96(2): 294.
  • Peña E, Olate E, Chorbadjian RA, Rosales IM, 2011. First Report of Alfalfa mosaic virus Infection in Viburnum tinus in Chile. Plant Disease, 95(9):1198.
  • Peterson JJ, Samac DA, Grau CR, 2018. First Report of Fusarium Wilt of Alfalfa Caused by Fusarium oxysporum f. sp. medicaginis in Wisconsin. Disease Notes. 102 (2): 447.
  • Petrovic D, Bulajic A, Stankovic I, Ignjatov M, Vujakovic M, Krstic B, 2010. Presence and distribution of pepper viruses in Serbia. Ratarstvo i Povrtarstvo. 47: 567–576.
  • Qin F, Liu D, Sun B, Ruan L, Ma Z, Wang H, 2016. Identification of Alfalfa Leaf Diseases Using Image Recognition Technology. PLoS ONE 11(12): e0168274. doi:10.1371/journal. Pone.0168274.
  • Radovic J, Sokolovic D, Markovic J, 2009. Alfalfa-most important perennial forage legume in animal husbandry. Biotechnology in Animal Husbandry. 25: 465-475.
  • Ragsdale DW, Radcliffe EB, DiFonzo CD, 2001. Epidemiology and feld control of PVY and PLRV. In: Virus and Virus-like Diseases of Potatoes and Production of Seed-potatoes. Eds. Loebenstein G., PH Berger, AA Brunt and RH Lawson. Huwer Academic Publishers, Dordrecht: 237-270.
  • Rahman F, Peaden RN, 1993. Incidence of Viruses on Alfalfa in Western North America. Plant Disease, 77: 160-162.
  • Regenmortel MHV, Pink L, 1981. Alfalfa mosaic virus In, " Handbook of Plant Virus Infections and Comparative Diagnosis." (Ed. Kurstak) Elsevier, Amsterdam pp. 415-422.
  • Samac DA, Nix RJ, Oleson A, 1998. Transmission frequency of Clavibacter michiganensis subsp. insidiosus to alfalfa seed and identification of the bacterium by PCR. Plant Disease, 82: 1362-1367.
  • Sawalha HD, Mansour AN, 1996b. Incidence of Alfalfa mosaic virus in alfalfa fields in Jordan. Dirasat: Agricultural Sciences. 23: 81- 83.
  • Sertkaya G, Üremiş İ, Sertkaya E, 2013. Weeds as Reservoirs for Virus Diseases and Their Vectors in Potato Fields in Amik Plain-Turkey. 15th European Association for Potato Research (EAPR) Virology Section Meeting. (28-31 May, Antalya, Turkey): 29.
  • Sertkaya G, Çarpar H, Sertkaya E, 2017.Detection of Alfalfa mosaic virus (AMV) in Potato Production Areas in Hatay Province of Turkey. Journal of the Institute of Science and Technology (JIST), 7(1): 23-29.
  • Shah DA, Dillard HR, Mazumdar-Leighton S, Gonsalves D, Nault BA, 2006. Incidence, spatial patterns, and associations among viruses in snap bean and alfalfa in New York. Plant Disease, 90: 203-210.
  • Sisterson MS, Thammiraju SR, Patterson KL, Groves RL, Daane K, 2010. Epidemiology of Diseases Caused by Xylella fastidiosa in California: Evaluation of Alfalfa as a Source of Vectors and Inocula, Plant Disease. 94 (7): 827-834.
  • Small E, 2011. Alfalfa and relatives: Evolution and classification of Medicago. NRC Research Press, Canada.
  • Soya H, Avcioglu R, Geren H, 2004. Forage crops, Hasad Press, Turkey, 223p (in Turkish).
  • Staniulis J, 1994. Ankštinių augalų virusinių ir geltos tipo ligų sukėlėjai Lietuvoje (Gamtos mokslų habilitacinis darbas). Vilnius.
  • Šutic DD, Ford RE, Tošic MT, 1999. Handbook of plant virus diseases. Washington: CRC Press.
  • Svoboda J, Svobodová-Leišová, 2012. Occurrence of viruses on pepper plantations in the Czech Republic – Short communication. Horticultural Science, (Prague). 39: 139- 143.
  • Taha EA, 2015. A study on nectar and pollen sources for honeybee Apis mellifera L. in AlAhsa Saudi Arabia. Journal of Entomology and Zoology Studies, 3(:)272–277.
  • Tenllado F, Bol JF, 2000. Genetic detection of the multiple functions of Alfalfa mosaic virus coat protein in viral RNA replication, encapsidation, and movement. Virology. 268: 29- 40.
  • Thresh JM, 1981. The Role of Weeds and Wild Plants in the Epidemiology of Plant Virus Diseases. In: "Pests Pathogens and Vegetation", (Ed.): Thresh, J. M.. Pitman Publications, London, UK, PP. 53–70.
  • Trucco V, Soledad B, Nicolas B, Sergio L, Fabián G, 2016. Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields. The European Journal of Plant Pathology,146. 10.1007/s10658-016-0899-5.
  • Turan N, Celen AS, Ozyazıcı MA, 2017. Yield and quality characteristics of some alfalfa (Medicago sativa L.) varietes grown in the Eastern Turkey. Turkish Journal of Field Crops, 22(2): 160-165
  • TÜİK, 2017. Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim Tarihi, 20.12.2018).
  • TÜİK, 2017a. Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim Tarihi, 20.12.2018).
  • Van Dun CMP, Bol JF, Van VlotenDoting L, 1987. Expression of Alfalfa mosaic virus and Tobacco Rattle Virus Coat Protein Genes in Transgenic Tobacco Plants. Virology, 159: 299-305.
  • Vasić TP, Trkulja VR, Rajčević BM, Živković SP, Anđelković SZ, Marković JP, 2011. Molecular And Morphological Determination of Colletotrichum trifolii Isolates Originating from Alfalfa. Proceedings of the National Academy of Sciences, Matica Srpska Novi Sad. 120: 195—202.
  • Vozelj N, Petrovic N, Novak MP, Tusek M, Mavric I, Ravnikar M, 2003. The most frequent viruses on selected ornamental plants and vegetables in Slovenia. In: Zbornik Predavanj in Referatov 6. Slovenskega Posvetovanje o Varstvu Rastlin, March 4–6, 2003. Zrece, Slovenia: 300- 304.
  • Weimer JL, 1931. Alfalfa mosaic virus (type strain). CMI/AAB Description of Plant Viruses, No. 46.
  • Xu H, Nie J, 2006. Identification, characterization and molecular detection of Alfalfa mosaic virus in potato. Phytopathology. 96: 1237-1242.
  • Yardımcı N, Açıkgöz S, 1997. 1997. Erzurum Yöresi Yoncalarında, Mozayik Hastalığı Etmeninin Test Bitkileri İle Tanılanması. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 28(2): 195-205.
  • Zitikaitė I, Samuitienė M, 2008. Identifcation and some properties of Alfalfa mosaic alfamovirus isolated from naturally infected tomato cropbiologija. Biologija. 54 (2): 83–88.

Van İlinde Yoncada Saptanan Yonca mozaik virüs (AMV) İzolatlarının Kılıf Protein Genomunun Moleküler Karakterizasyonu

Yıl 2020, , 2366 - 2377, 15.12.2020

Mustafa Usta , Abdullah Güller

https://doi.org/10.21597/jist.719099
Cited By: 3

Öz

Van ilinde yetiştiriciliği yapılan yonca (Medicago sativa L.) bitkilerinde 2019 yılında virüslerin oluşturduğu simptomlara benzer belirtiler görülmüştür. Gözlenen belirtiler arasında cüceleşme, yapraklarda mozaik deseni ve sararma, rozetlenme ve küçük yaprak oluşumu yer almaktadır. Simptom gösteren ve göstermeyen bitkilerden toplanan 19 yonca yaprak örneğine, virüs genomunu tespit edebilmek için uygun primer çiftleri ile RT-PCR testi uygulanmıştır. Yonca yaprağı örneklerinden şiddetli belirtiye sahip altısı beklenen büyüklükte 700 bp DNA band oluşturmuştur. Bunlar arasından rastgele seçilen ikisi bir plazmid vektörüne klonlanmıştır. Elde edilen rekombinant plazmidler her iki yönde dizilenmiştir. Dizi analizi sonuçlarına göre infekteli yoncalardaki virüsün Yonca mozaik virüs olduğu açığa çıkarılmıştır. Dizi bilgileri MT210179 ve MT210178 erişim numaraları ile gen bankasına yüklenmiştir ve sırasıyla Alakoy Y9 ve Alakoy Y1 olarak isimlendirilmiştir. Her iki dizi için gen bankasına kayıtlı 16 AMV dizisi ile oluşturulan filogenetik ağaca göre, her iki izolat da nükleotit düzeyinde ABD, Brezilya ve Puglia izolatları ile en yüksek benzerlik ve Güney Kore izolatı ile en düşük benzerlik oranı göstermiştir. Ayrıca her iki dizinin birbirleri arasında, 7 nükleotit değişikliği ile %98.45 oranında nükleotit benzerliği gösterdiği ortaya çıkarılmıştır. Gerçekleştirilen litaratür tarama çalışmalarına göre, bu çalışma Türkiye'nin Van ilinde yetiştirilen yonca bitkilerindeki Yonca mozaik virüsü (AMV)’ nün ilk raporu ve moleküler analizidir.

Anahtar Kelimeler

Dizileme filogenetik analiz RT-PCR karakterizasyon yonca

Kaynakça

  • Abdalla OA, Al-Shahwan IM, AL-Saleh MA, Amer MA, 2019. Molecular characterization of Alfalfa mosaic virus (AMV) isolates in alfalfa and other plant species in different regions in Saudi Arabia. European Journal of Plant Pathology,156: 603–613.
  • Alan B, Crabtree K, Dallwitz M, Gibbs A, Watson L, 1996. Viruses of Plants. Description and Lists from the VIDE Database. CAB International, UK,1484 PP.
  • Alfaro-Fernández A, Sánchez-Navarro J A, Landeira M, Font MI, Hernández-Llópis D, Pallás V, 2016. Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and Tomato yellow leaf curl sardinia virus. Journal of Plant Pathology, 98 (2): 245-254.
  • Alhudaib KA, 2019. Serological and Molecular Detection of Alfalfa mosaic virus in the Major Potato Growing Areas of Saudi Arabia. Scientifc Journal of King Faisal University (Basic and Applied Sciences), 20 (1): 73- 82.
  • Ali H, Gholam K, 2011. Detection and Identification of Clavibacter michiganensis subsp. insidiosus inducing Alfalfa Wilt Disease Using Specific PCR and Physiological Methods in Iran. Research Journal of Chemistry and Environment, 15 (2): 486-491.
  • Al-Saleh MA, Amer MA, 2013. Biological and Molecular variability of Alfalfa mosaic virus affecting alfalfa crop in riyadh region. The Plant Pathology Journal, 29(4) : 410-417.
  • Al-Shahwan IM, Abdalla OA, Al-Saleh MA, Amer MA, 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
  • Anonim, 2014. T. C. Gıda Tarım ve Hayvancılık Bakanlığı, Tarla Bitkileri Araştırmaları Dairesi Başkanlığı, Çayır Mera ve Yem Bitkileri Araştırmaları Çalışma Grubu Raporu. https://www.tarimorman.gov.tr/TAGEM/Belgeler/SUNULAR/%C3%87MYB_Birg%C3%B Cl%20G%C3%BCner.PDF (Erişim Tarihi: 26.12.2018).
  • Anonymous 2017. Bugem Activities. T. C. Ministry of Food, Agriculture and Livestock, General Directorate of Plant Production, March 2017 (in Turkish).
  • Arlı-Sökmen M, Mennan H, Sevik MA, Ecevit O, 2005. Occurence of viruses in field-grown pepper crops and some of their reservoir weeds hosts in Samsun, Turkey. Phytoparasitica, 33(4), 347-358.
  • Bailiss KW, Ollennu LAA. 1986. Effect of Alfalfa mosaic virus isolates on forage yield of lucern (Medicago sativa) in Britain. Plant Pathology, 35: 162- 168.
  • Balasubramaniam M, Kim BS, Hutchens-Williams HM, Loesch-Fries LS, 2014. The photosystem II oxygen-evolving complex protein PsbP interacts with the coat protein of Alfalfa mosaic virus and inhibits virus replication. Molecular Plant-Microbe Interactions, 27(10): 1107- 1118.
  • Bol JF, 2003. Alfalfa mosaic virus: Coat protein dependent initiation of infection. Molecular Plant Pathology, 4(1): 1-8.
  • Brunt A, Crabtree K, Gibbs A, 1990. Viruses of Tropical Plants. CAB International, London.
  • Buzkan N, Demir M, Öztekin V, Mart C, Çağlar BK, Yilmaz MA, 2006. Evaluation of the status of capsicum viruses in the main growing regions of Turkey. Bulletin OEPP, 36(1): 15–19.
  • Chod J, Chodová D, Kočova M, Jokeš M, 1994. Occurrence of Alfalfa mosaic virus on red pepper (Capsicum annuum L.) and investigation of susceptibility of some cultivars with respect to Hill’s reaction activity and chlorophyll content. Zahradnictví, 21: 27–36.
  • Cook AA, Wilton AC, 1984. Alfalfa enation virus in the Kingdom of Saudi Arabia. FAO, Plant Protection Bulletin, 32: 139-140.
  • Çalı S, 1990. Orta Anadolu Bölgesi Yonca Ekim Alanlarında Görülen Virüs Enfeksiyonlarının Saptanması, Yabancı Otlar ve Tohumla Taşınması Üzerine Araştırmalar. Adana Zirai Mücadele Araştırma Ens. Müdürlüğü, Araştırma Yayınlan Serisi, Yayın No: 72.
  • Çetinkıran AD, Baloğlu S, 2011. Detection of the Alfalfa mosaic virus in pepper fields in Adana and Mersin. Ç.Ü Fen ve Mühendislik Bilimleri Dergisi, 26 (2): 89- 98.
  • Çulal Kılıç H, Yardımcı N, 2015. Occurrence of Alfalfa mosaic virus (AMV) infecting bean crop in Burdur Province, Turkey. Asian Journal of Agriculture and Food Sciences, 3(2): 173- 177.
  • Dale LM, Rotar I, Thewis A, Vidican R, Florian V, Ciure A, 2012. Determination of alfalfa crude fiber, ndf, adf and lignin content by nir spectrometry. Lucrări Ştiintifice, vol. 55, seria Agronomie, p: 245-249.
  • Demir M, 2005. Kahramanmaraş’ta Yetiştirilen Kırmızı Biberlerde Yaprakbiti İle Taşınan Virüslerin Saptanması. Kahramanmaraş Sütçü İmam Üniversitesi. Fen Bilimleri Enstitüsü. Bitki Koruma Anabilimdalı. Yüksek Lisans Tezi, Kahramanmaraş. 25s
  • El Helaly HS, Ahmed AA. Awad MA, Soliman AM, 2012. Biological and Molecular Characterization of Potato infecting Alfalfa mosaic virus in Egypt. International Journal of Virology, 8: 106- 113.
  • Erdiller G, Lesemann DE, 1985. Türkiye İçin Yeni Bir Virüs Hastalığı "Alfalfa Mosaic Virus". Ankara Üniv. Ziraat Fakültesi Yıllığı, 35: 20-25.
  • Fidan H, Adak NA, Konuksal A, Akerzurumlu E, Yılmaz MA, 2012. Occurrence of Alfalfa mosaic virus (AMV) diseases on potato crops in Northern Cyprus. Acta Horticulturae, 960: 341-346.
  • Foissac X, Svanella-Dumas L, Gentit P, Dulucq MJ, Candresse T, 2001. Polyvalent detection of fruit tree Tricho-,Capillo- and Foveaviruses by nested RT-PCR using degenerated and inosine-containing primers (PDO RT-PCR). Acta Horticulturae, 550: 37-44.
  • Forster RLS, Morris-Krsinich BAM, Musgrave DR, 1985. Incidence of Alfalfa mosaic virus, Lucerne Australian latent virus, and Lucerne transient streak virus in lucerne crops in the North Island of New Zealand New. New Zealand Journal of Agricultural Research, 28: 279-282.
  • Frencel I, Pospieszny H, 1979. Viruses in natural infections of yellow lupin (Lupinus luteus L.) in Poland. III. Alfalfa mosaic virus (AMV). Acta Phytopathologica Academiae Scientarum Hungaricae, 14(3–4): 269- 278.
  • Frosheiser FI, 1974. Alfalfa mosaic virus Transmission to Seed through Alfalfa Gametes and Longevity in Alfalfa Seed. Phytopathology, 64: 102 – 105.
  • Garran J, Gibbs A,. 1982. Studies on Alfalfa mosaic virus and alfalfa aphids. Australian Journal of Agricultural Research, 33: 657-664.
  • Geren H, Kir B, Demiroglu G, Kavut YT, 2009. Effects of different soil textures on the yield and chemical composition of alfalfa (Medicago sativa L.) cultivars under mediterranean climate conditions. Asian Journal of Chemistry, 21 (7) : 5517-5522.
  • Güzel Ö, Arlı-Sökmen M, 2003. Determination of some viruses infecting common bean (Phaseoulus vulgaris L.) and their incidences in seed lots in Samsun Province. The Journal of Turkish Phytopathology, 32 (2): 99-106.
  • Hajimorad MR, Francki RIB, 1988. Alfalfa mosaic virus isolates from lucerne in South Australia: Biological variability and antigenic similarity. The Annals of Applied Biology, 113: 45–54.
  • Hemmati K, Mclean DL, 1977. Gamete-seed Transmission of Alfalfa mosaic virus and its effect of Seed Germination and Yield in Alfalfa Plants. Phytopathology, 67: 576-579.
  • Hiruki C, Hampton RO, 1990. Alfalfa Mosaic. In: "Compendium of Alfalfa Diseases", (Eds.): Stuteville, D. L. and Erwin, D. C.. 2nd Edition, APS Press, St. Paul, 54 PP.
  • Jaspars EM, Bos L, 1980. Alfalfa mosaic virus. No. 229. In: Descriptions of plant viruses. Common wealth Mycology Institute/Association of Applied Biologists, Kew, England.
  • Kavut YT, Avcıoglu R, 2015. Yield and quality performances of various alfalfa (Medicago sativa L.) cultivars in different soil textures in a Mediterranean environment. Turkish Journal of Field Crops, 20(1): 65-71.
  • Mallor C, Luis-Arteaga M, Cambra MA, Fernandez-Cavada S, 2002. Disease Notes, 86: 698.
  • Mangeli F, Massumi H, Alipour F, Maddahian M, Heydarnejad J, Hosseinipour A, Amid-Motlagh MH, Azizizadeh M, Varsani A, 2019. Molecular and partial biological characterization of the coat protein sequences of Iranian Alfalfa mosaic virus isolates. Journal of Plant Pathology, 101: 73-742.
  • Martínez-Priego L, Córdoba MC, Jordá C, 2004. First Report of Alfalfa mosaic virus in Lavandula officinalis. Plant Disease, 88(8): 908.
  • Massumi H, Maddahian M, Heydarnejad J, Hosseini Pour A, Farahmand A, 2012. Incidence of Viruses Infecting Alfalfa in the Southeast and Central Regions of Iran. Journal of Agricultural Science and Technology, 14: 1141-1148.
  • McLaughlin MR, Boykin D, 1988. Virus Diseases of Seven Species of Forage Legumes in the Southeastern United States. Plant Disease, 72: 539-542.
  • Monteros MJ, Bouton JH, 2009.The future of alfalfa and forage crops In: Proceedings, Western Alfalfa & Forage Conference, December 2–4, Reno, Nevada.
  • Moreira AG, Kitajima EW, Rezende JA, 2010. Identification and partial characterization of a Carica papaya-infecting isolate of Alfalfa mosaic virus in Brazil. The Journal of General Plant Pathology, 76: 172- 175.
  • Moreira A, Fageria NK, 2010. Liming influence on soil chemical properties, nutritional status and yield of alfalfa grown in acid soil. The Revista Brasileira de Ciência do Solo (RBCS) , 34, p:1231-1239.
  • Mughal SM, Zadjali AD, Matrooshi AR, 2003. Occurrence, distribution and some properties of Alfalfa mosaic alfamovirus in the Sultanate of Oman. Pakistan Journal of Agricultural Sciences, 40(1-2): 67-73.
  • Nemchinov LG, Grinstead SC, Mollov DS, 2017. Alfalfa virus S, a new species in the family Alphaflexiviridae. Plos One, 12(5): 1-11.
  • Özdemir S, Erilmez, S. 2007. Denizli ilinde yetiştirilen biber, patlıcan ve marul üretim alanlarında bazı viral etmenlerin saptanması. Türkiye II. Bitki Koruma Kongresi Bildirileri.27-29 Ağustos Isparta. 114s.
  • Ozdemir S, Erilmez S, Paylan IC, 2011. First report of Alfalfa mosaic virus in eggplant in Turkey. The Journal of Plant Pathology (JPP), 93(4): 63-89.
  • Özdemir S, Erilmez S, Paylan Cİ, 2011. Ege Bölgesi Patates Üretim Alanlarında Alfalfa mosaic alfamovirus’ün Serolojik ve Moleküler Olarak Tanılanması. Türkiye IV. Bitki Koruma Kongresi Bildirileri: 410.
  • Paliwal YC, 1982. Virus diseases of alfalfa and biology of Alfalfa mosaic virus in Ontario and western Quebec. Canadian Journal of Plant Pathology, 4: 175- 179.
  • Palumbo JD, Phillips DA, Kado CI, 1998. Characterization of a new Agrobacterium tumefaciens strain from alfalfa (Medicago sativa L.). 1998. Archives of Microbiology, 169: 381–386.
  • Parrella G, Acanfora N, Orílio A, Navas-Castillo J, 2011. Complete nucleotide sequence of a Spanish isolate of Alfalfa mosaic virus: Evidence for additional genetic variability. Archives of Virology, 156, 1049–1052.
  • Parrella G, Cavicchi L, Bellardi MG, 2012. First Record of Alfalfa mosaic virus in Teucrium fruticans in Italy. Disease Notes. 96(2): 294.
  • Peña E, Olate E, Chorbadjian RA, Rosales IM, 2011. First Report of Alfalfa mosaic virus Infection in Viburnum tinus in Chile. Plant Disease, 95(9):1198.
  • Peterson JJ, Samac DA, Grau CR, 2018. First Report of Fusarium Wilt of Alfalfa Caused by Fusarium oxysporum f. sp. medicaginis in Wisconsin. Disease Notes. 102 (2): 447.
  • Petrovic D, Bulajic A, Stankovic I, Ignjatov M, Vujakovic M, Krstic B, 2010. Presence and distribution of pepper viruses in Serbia. Ratarstvo i Povrtarstvo. 47: 567–576.
  • Qin F, Liu D, Sun B, Ruan L, Ma Z, Wang H, 2016. Identification of Alfalfa Leaf Diseases Using Image Recognition Technology. PLoS ONE 11(12): e0168274. doi:10.1371/journal. Pone.0168274.
  • Radovic J, Sokolovic D, Markovic J, 2009. Alfalfa-most important perennial forage legume in animal husbandry. Biotechnology in Animal Husbandry. 25: 465-475.
  • Ragsdale DW, Radcliffe EB, DiFonzo CD, 2001. Epidemiology and feld control of PVY and PLRV. In: Virus and Virus-like Diseases of Potatoes and Production of Seed-potatoes. Eds. Loebenstein G., PH Berger, AA Brunt and RH Lawson. Huwer Academic Publishers, Dordrecht: 237-270.
  • Rahman F, Peaden RN, 1993. Incidence of Viruses on Alfalfa in Western North America. Plant Disease, 77: 160-162.
  • Regenmortel MHV, Pink L, 1981. Alfalfa mosaic virus In, " Handbook of Plant Virus Infections and Comparative Diagnosis." (Ed. Kurstak) Elsevier, Amsterdam pp. 415-422.
  • Samac DA, Nix RJ, Oleson A, 1998. Transmission frequency of Clavibacter michiganensis subsp. insidiosus to alfalfa seed and identification of the bacterium by PCR. Plant Disease, 82: 1362-1367.
  • Sawalha HD, Mansour AN, 1996b. Incidence of Alfalfa mosaic virus in alfalfa fields in Jordan. Dirasat: Agricultural Sciences. 23: 81- 83.
  • Sertkaya G, Üremiş İ, Sertkaya E, 2013. Weeds as Reservoirs for Virus Diseases and Their Vectors in Potato Fields in Amik Plain-Turkey. 15th European Association for Potato Research (EAPR) Virology Section Meeting. (28-31 May, Antalya, Turkey): 29.
  • Sertkaya G, Çarpar H, Sertkaya E, 2017.Detection of Alfalfa mosaic virus (AMV) in Potato Production Areas in Hatay Province of Turkey. Journal of the Institute of Science and Technology (JIST), 7(1): 23-29.
  • Shah DA, Dillard HR, Mazumdar-Leighton S, Gonsalves D, Nault BA, 2006. Incidence, spatial patterns, and associations among viruses in snap bean and alfalfa in New York. Plant Disease, 90: 203-210.
  • Sisterson MS, Thammiraju SR, Patterson KL, Groves RL, Daane K, 2010. Epidemiology of Diseases Caused by Xylella fastidiosa in California: Evaluation of Alfalfa as a Source of Vectors and Inocula, Plant Disease. 94 (7): 827-834.
  • Small E, 2011. Alfalfa and relatives: Evolution and classification of Medicago. NRC Research Press, Canada.
  • Soya H, Avcioglu R, Geren H, 2004. Forage crops, Hasad Press, Turkey, 223p (in Turkish).
  • Staniulis J, 1994. Ankštinių augalų virusinių ir geltos tipo ligų sukėlėjai Lietuvoje (Gamtos mokslų habilitacinis darbas). Vilnius.
  • Šutic DD, Ford RE, Tošic MT, 1999. Handbook of plant virus diseases. Washington: CRC Press.
  • Svoboda J, Svobodová-Leišová, 2012. Occurrence of viruses on pepper plantations in the Czech Republic – Short communication. Horticultural Science, (Prague). 39: 139- 143.
  • Taha EA, 2015. A study on nectar and pollen sources for honeybee Apis mellifera L. in AlAhsa Saudi Arabia. Journal of Entomology and Zoology Studies, 3(:)272–277.
  • Tenllado F, Bol JF, 2000. Genetic detection of the multiple functions of Alfalfa mosaic virus coat protein in viral RNA replication, encapsidation, and movement. Virology. 268: 29- 40.
  • Thresh JM, 1981. The Role of Weeds and Wild Plants in the Epidemiology of Plant Virus Diseases. In: "Pests Pathogens and Vegetation", (Ed.): Thresh, J. M.. Pitman Publications, London, UK, PP. 53–70.
  • Trucco V, Soledad B, Nicolas B, Sergio L, Fabián G, 2016. Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields. The European Journal of Plant Pathology,146. 10.1007/s10658-016-0899-5.
  • Turan N, Celen AS, Ozyazıcı MA, 2017. Yield and quality characteristics of some alfalfa (Medicago sativa L.) varietes grown in the Eastern Turkey. Turkish Journal of Field Crops, 22(2): 160-165
  • TÜİK, 2017. Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim Tarihi, 20.12.2018).
  • TÜİK, 2017a. Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim Tarihi, 20.12.2018).
  • Van Dun CMP, Bol JF, Van VlotenDoting L, 1987. Expression of Alfalfa mosaic virus and Tobacco Rattle Virus Coat Protein Genes in Transgenic Tobacco Plants. Virology, 159: 299-305.
  • Vasić TP, Trkulja VR, Rajčević BM, Živković SP, Anđelković SZ, Marković JP, 2011. Molecular And Morphological Determination of Colletotrichum trifolii Isolates Originating from Alfalfa. Proceedings of the National Academy of Sciences, Matica Srpska Novi Sad. 120: 195—202.
  • Vozelj N, Petrovic N, Novak MP, Tusek M, Mavric I, Ravnikar M, 2003. The most frequent viruses on selected ornamental plants and vegetables in Slovenia. In: Zbornik Predavanj in Referatov 6. Slovenskega Posvetovanje o Varstvu Rastlin, March 4–6, 2003. Zrece, Slovenia: 300- 304.
  • Weimer JL, 1931. Alfalfa mosaic virus (type strain). CMI/AAB Description of Plant Viruses, No. 46.
  • Xu H, Nie J, 2006. Identification, characterization and molecular detection of Alfalfa mosaic virus in potato. Phytopathology. 96: 1237-1242.
  • Yardımcı N, Açıkgöz S, 1997. 1997. Erzurum Yöresi Yoncalarında, Mozayik Hastalığı Etmeninin Test Bitkileri İle Tanılanması. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 28(2): 195-205.
  • Zitikaitė I, Samuitienė M, 2008. Identifcation and some properties of Alfalfa mosaic alfamovirus isolated from naturally infected tomato cropbiologija. Biologija. 54 (2): 83–88.
Toplam 87 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Agronomi
Bölüm Bitki Koruma / Plant Protection
Yazarlar

Mustafa Usta 0000-0002-3940-2774

Abdullah Güller 0000-0003-3887-4208

Yayımlanma Tarihi 15 Aralık 2020
Gönderilme Tarihi 13 Nisan 2020
Kabul Tarihi 4 Temmuz 2020
Yayımlandığı Sayı Yıl 2020

Kaynak Göster

APA Usta, M., & Güller, A. (2020). Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology, 10(4), 2366-2377. https://doi.org/10.21597/jist.719099
AMA Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. Aralık 2020;10(4):2366-2377. doi:10.21597/jist.719099
Chicago Usta, Mustafa, ve Abdullah Güller. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology 10, sy. 4 (Aralık 2020): 2366-77. https://doi.org/10.21597/jist.719099.
EndNote Usta M, Güller A (01 Aralık 2020) Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology 10 4 2366–2377.
IEEE M. Usta ve A. Güller, “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”, Iğdır Üniv. Fen Bil Enst. Der., c. 10, sy. 4, ss. 2366–2377, 2020, doi: 10.21597/jist.719099.
ISNAD Usta, Mustafa - Güller, Abdullah. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology 10/4 (Aralık 2020), 2366-2377. https://doi.org/10.21597/jist.719099.
JAMA Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. 2020;10:2366–2377.
MLA Usta, Mustafa ve Abdullah Güller. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology, c. 10, sy. 4, 2020, ss. 2366-77, doi:10.21597/jist.719099.
Vancouver Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. 2020;10(4):2366-77.

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